Protective Effect of Methanol Extract of Swietenia macrophylla Seedson Oxidative States Associated with Streptozotocin Induced Diabetic Rats

Anup Maiti,Saikat Dewanjee,Mintu Kundu,Subhash C. Mandal 한국생약학회 2007 Natural Product Sciences Vol.13 No.4

methanol extract of seeds of Swietenia macrophyllaantidiabetic activity in streptozotocin induced diabetic rats. It was principally aimed to correlate the eficaciousrole of MESM on reduction of oxidative state associated with diabetes. The extract was found to be potentantidiabetic evidenced by significant reduction of blood glucose level in diabetic rats (47.96% reduction of bloodglucose level, at 300 mg/kg, on day 10). It was found that, MESM at 300 mg/kg, significantly decreased TBARS(35.03 and 22.22%) whilst increased GSH (86.75 and 31.45%), SOD (93.05 and 45.88%) and CAT (56.99 and68.46%) levels in liver and kidney respectively in diabetic rats. Keywords Antioxidant, Swietenia macrophylla

Reduced Expression of Limd1 in Ulcerative Oral Epithelium Associated with Tobacco and Areca Nut

Maiti, Guru Prasad,Ghosh, Amlan,Chatterjee, Ramdas,Roy, Anup,Sharp, Tyson V.,Roychoudhury, Susanta,Panda, Chinmay Kumar Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Purpose: The aim of this study was to cast light on initiating molecular events associated with the development of premalignant oral lesions induced by tobacco and/or areca nut. Method: Immunohistochemical analyses of cell cycle regulatory proteins (LIMD1, RBSP3, p16, RB, phosphorylated RB, p53), EGFR and SH3GL2 (EGFR associated protein) were performed with inflammatory/ulcerative epithelium and adjacent hyperplastic/mild dysplastic lesions. Results: No change in expression of the proteins was seen in inflammatory epithelium. Reduced nuclear expression of LIMD1 was evident in ulcerative epithelium. In hyperplastic lesions, reduced expression of RBSP3, p16, SH3GL2 and overexpression of p-RB and EGFR were apparent. Reduced nuclear expression of p53 was observed in mild dysplastic lesions. Conclusion: Our data suggest that inactivation of LIMD1 in ulcerative epithelium might predispose the tissues to alterations of other cell cycle regulatory and EGFR signaling proteins needed for the development of premalignant oral lesions.

Hypoglycemic activity of diospyros peregrina fruits in diabetic rats

Saikat Dewanjee,Anup Maiti,Mintu Kundu,Subhash C Mandal 경희대학교 융합한의과학연구소 2008 Oriental Pharmacy and Experimental Medicine Vol.8 No.3

Diospyros peregrina Gurke. (Ebenaceae) is a small middle sized tree grows luxuriantly in the plains of costal West Bengal, India. The objective of the study was to explore the antidiabetic activity of methanol extract of matured fruits of Diospyros peregrina to substantiate the folklore claim of traditional practitioners. It was also aimed to establish correlation with reduction of oxidative state associated with diabetes. Methanol extract of matured fruits of Diospyros peregrina was administered orally at doses of 150 and 300 mg/kg body weight for 12 consecutive days to normal and streptozotocin induced diabetic rats. Fasting blood glucose level was estimated in both normal and diabetic rats while serum lipid profiles, liver glycogen level and pancreatic thiobarbituric acid reactive substances (TBARS) were evaluated for diabetic rats. Initial and final changes in body weight were also recorded. Oral glucose tolerance test was performed during the course of study. Experimental findings showed significant antidiabetic potential of extract in term of reduction of fasting blood glucose level of both normal and diabetic rats. It was found that extract at the dose of 300 mg/kg body weight is more effective and percentage reduction (55.64) of elevated blood glucose level is comparable to that of standard drug glibenclamide (60.60) at a dose of 10 mg/kg body weight. Observed data found statistically significant in reduction of serum lipid and pancreatic TBARS levels whilst improvement was observed in liver glycogen level and body weight profiles in extract treated diabetic rats. Diospyros peregrina Gurke. (Ebenaceae) is a small middle sized tree grows luxuriantly in the plains of costal West Bengal, India. The objective of the study was to explore the antidiabetic activity of methanol extract of matured fruits of Diospyros peregrina to substantiate the folklore claim of traditional practitioners. It was also aimed to establish correlation with reduction of oxidative state associated with diabetes. Methanol extract of matured fruits of Diospyros peregrina was administered orally at doses of 150 and 300 mg/kg body weight for 12 consecutive days to normal and streptozotocin induced diabetic rats. Fasting blood glucose level was estimated in both normal and diabetic rats while serum lipid profiles, liver glycogen level and pancreatic thiobarbituric acid reactive substances (TBARS) were evaluated for diabetic rats. Initial and final changes in body weight were also recorded. Oral glucose tolerance test was performed during the course of study. Experimental findings showed significant antidiabetic potential of extract in term of reduction of fasting blood glucose level of both normal and diabetic rats. It was found that extract at the dose of 300 mg/kg body weight is more effective and percentage reduction (55.64) of elevated blood glucose level is comparable to that of standard drug glibenclamide (60.60) at a dose of 10 mg/kg body weight. Observed data found statistically significant in reduction of serum lipid and pancreatic TBARS levels whilst improvement was observed in liver glycogen level and body weight profiles in extract treated diabetic rats.

Hypoglycemic activity of diospyros peregrina fruits in diabetic rats

Dewanjee, Saikat,Maiti, Anup,Kundu, Mintu,Mandal, Subhash C Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.3

Diospyros peregrina Gurke. (Ebenaceae) is a small middle sized tree grows luxuriantly in the plains of costal West Bengal, India. The objective of the study was to explore the antidiabetic activity of methanol extract of matured fruits of Diospyros peregrina to substantiate the folklore claim of traditional practitioners. It was also aimed to establish correlation with reduction of oxidative state associated with diabetes. Methanol extract of matured fruits of Diospyros peregrina was administered orally at doses of 150 and 300 mg/kg body weight for 12 consecutive days to normal and streptozotocin induced diabetic rats. Fasting blood glucose level was estimated in both normal and diabetic rats while serum lipid profiles, liver glycogen level and pancreatic thiobarbituric acid reactive substances (TBARS) were evaluated for diabetic rats. Initial and final changes in body weight were also recorded. Oral glucose tolerance test was performed during the course of study. Experimental findings showed significant antidiabetic potential of extract in term of reduction of fasting blood glucose level of both normal and diabetic rats. It was found that extract at the dose of 300 mg/kg body weight is more effective and percentage reduction (55.64) of elevated blood glucose level is comparable to that of standard drug glibenclamide (60.60) at a dose of 10 mg/kg body weight. Observed data found statistically significant in reduction of serum lipid and pancreatic TBARS levels whilst improvement was observed in liver glycogen level and body weight profiles in extract treated diabetic rats.

Reduction of Proliferation and Induction of Apoptosis are Associated with Shrinkage of Head and Neck Squamous Cell Carcinoma due to Neoadjuvant Chemotherapy

Sarkar, Shreya,Maiti, Guru Prasad,Jha, Jayesh,Biswas, Jaydip,Roy, Anup,Roychoudhury, Susanta,Sharp, Tyson,Panda, Chinmay Kumar Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Background: Neoadjuvant chemotherapy (NACT) is a treatment modality whereby chemotherapy is used as the initial treatment of HNSCC in patients presenting with advanced cancer that cannot be treated by other means. It leads to shrinkage of tumours to an operable size without significant compromise to essential oro-facial organs of the patients. The molecular mechanisms behind shrinkage due to NACT is not well elucidated. Materials and Methods: Eleven pairs of primary HNSCCs and adjacent normal epithelium, before and after chemotherapy were screened for cell proliferation and apoptosis. This was followed by immunohistochemical analysis of some cell cycle (LIMD1, RBSP3, CDC25A, CCND1, cMYC, RB, pRB), DNA repair (MLH1, p53) and apoptosis (BAX, BCL2) associated proteins in the same set of samples. Results: Significant decrease in proliferation index and increase in apoptotic index was observed in post-therapy tumors compared to pre-therapy. Increase in the RB/pRB ratio, along with higher expression of RBSP3 and LIMD1 and lower expression of cMYC were observed in post-therapy tumours, while CCND1 and CDC25A remained unchanged. While MLH1 remained unchanged, p53 showed higher expression in post-therapy tumors, indicating inhibition of cell proliferation and induction of apoptosis. Increase in the BAX/BCL2 ratio was observed in post-therapy tumours, indicating up-regulation of apoptosis in response to therapy. Conclusions: Thus, modulation of the G1/S cell cycle regulatory proteins and apoptosis associated proteins might play an important role in tumour shrinkage due to NACT.

Surface Treated Jute Fiber Induced Foam Microstructure Development in Poly(lactic acid)/Jute Fiber Biocomposites and their Biodegradation Behavior

Mohammad Tahir Zafar,Sanjeev Kumar,Rajendra Kumar Singla,Saurindra Nath Maiti,Anup Kumar Ghosh 한국섬유공학회 2018 Fibers and polymers Vol.19 No.3

Poly(lactic acid) (PLA)/jute fiber biocomposites with: i) untreated jute fiber, ii) NaOH treated jute fiber, and iii) (NaOH+silane) treated jute fibers were prepared by melt extrusion process. Microcellular foaming of the injection molded samples was carried out by using single stage batch process. The effects of jute fiber content as well as that of matrix-fiber phase adhesion, in composites with surface treated jute fibers, on the foam microstructure were studied. Further, water absorption, thickness swelling, and biodegradation behavior of the foamed biocomposites were studied and correlated with their foam microstructures. It was observed that on increasing jute fiber content in PLA/JFU biocomposites, cell density increased from 6.5×107 to 8.1×107, while the cell size and expansion ratio decreased from 40 to 23 μm and 2.41 to 1.45, respectively. Again, on increasing the extent of the jute fiber surface treatment in the biocomposites, cell size and expansion ratio increased from 40 to 78 μm and 2.41 to 2.80 respectively. This study also revealed that the rate of biodegradation accelerated with increase in the jute fiber content in the biocomposites while the same retarded with increase in the extent of jute fiber surface treatment.