조직배양에 의한 위도상사화(Lycoris uydoensis M. Kim)의 효율적 기내번식

고정애 ( Jeong Ae Ko ),김인숙 ( In Sook Kim ),정공수 ( Gong Soo Jeong ),최정란 ( Jeong Ran Choi ) 전북대학교 농업과학기술연구소 2012 농업생명과학연구 Vol.43 No.2

This study was conducted to establish an effective in vitro mass propagation system using leaf and bulb scale explant sources of Lycoris uydoensis M. Kim. For the bulb scale culture, the bulbs treated at 5℃ for 60 days were dissected into three parts according to bulb height (upper, middle, and bottom), and allowed for a culture on the MS medium with 0.1 mg ? L-1 2,4-D plus 1.0 mg ? L-1 BA, 0.5 mg ? L-1 NAA plus 1.0 mg ? L-1 BA, and 3.0 mg ? L-1 IBA plus 3.0 mg ? L-1 BA. Regardless of plant hormone treatments, the bottom part of the bulb including the disk proved to be most effective in forming the callus and bulblets. Callus was readily produced on the young leaf segment and bulb scale tissues, whereas, bulblets and shoots were formed only on bulb scale explants. Bulb scales with disk appeared to the best optimal explant for in vitro propagation. Two distinct pathways, either organogenesis through callus or direct bulblet formation, could be recognized in bulb scale culture. Bulblet formation and shoot organogenesis from the bulb scales with disk were effectively induced up to the 60%~100% on the MS medium supplemented with 0.1 mg ? L-1 2,4-D plus 1.0 mg ? L-1 BA, 0.5 mg ? L-1 NAA plus 1.0 mg ? L-1 BA, and 3.0 mg ? L-1 IBA와 3.0 mg ? L-1 BA within 30 days of culture. On MS media supplemented with 0.1 mg ? L-1 2,4-D plus 1.0 mg ? L-1 BA or 3.0 mg ? L-1 IBA와 3.0 mg ? L-1 BA were more effective than 0.5 mg ? L-1 NAA plus 1.0 mg ? L-1 BA for induced and maintained a large number of bulblets. Six percent of sucrose enhanced bulblet formation and expanded the bulblets. Especially, adding 6% of sucrose on MS media supplemented with 3.0 mg ? L-1 IBA와 3.0 mg ? L-1 BA was one or two times of bulblets expanted than on MS mediun with 0.1 mg ? L-1 2,4-D plus 1.0 mg ? L-1 BA plus 6% of sucrose. Plantlet regeneration was successfully achieved from bulb scale-derived callus, via shoot bud induction or direct bulblet formation.

전통누룩 진균류를 이용한 입국의 제조 및 입국곰팡이의 동정

김재호 ( Jae Ho Kim ),권영희 ( Young Hee Kwon ),이애란 ( Ae Ran Lee ),김혜련 ( Hye Ryun Kim ),안병학 ( Byung Hak Ahn ) 한국균학회 2012 韓國菌學會誌 Vol.40 No.4

Various koji were prepared by fungi isolated from traditional nuruk and their quality characteristics were investigated. Acidity and saccharification power of their koji were ranged in 5.0~6.8 and 128sp~241sp. Nine fungi which were showed good quality and sensory evaluation were identified by analysis of their nucleotide sequences with PCR-amplified 18S rDNA internal transcribed spacer-1(ITS-1) and ITS-4 genes. Among them, six strains were identified as Aspergillus oryzae and the other strains were identified as Mycocladus corymbiferus, Rhizopus oryzae, Lichtheimia corymbifera.

Structural nature of chemically inequivalent borons in the nonlinear optical material β-BaB₂O₄ studied using ¹¹B MAS NMR and ¹¹B single-crystal NMR

Kim, Woo Young,Lim, Ae Ran Korean Magnetic Resonance Society 2013 Journal of the Korean Magnetic Resonance Society Vol.17 No.2

Detailed information about the structural nature of three-coordinate borons in ${\beta}-BaB_2O_4$ is obtained through $^{11}B$ MAS NMR and $^{11}B$ single-crystal NMR. The three-coordinate $BO_3$ of the two borons B(1) and B(2) in ${\beta}-BaB_2O_4$ were distinguished. The spin-lattice relaxation time in the laboratory frame $T_1$ for B(1) and B(2) slowly decreases with increasing temperature, whereas the spin-lattice relaxation time in the rotating frame $T_{1{\rho}}$ for B(1) and B(2), which differs from $T_1$, is nearly constant. The B(1) and B(2) of the two types were distinguished by $^{11}B$ MAS NMR and $^{11}B$ single-crystal NMR.

Enhancement of Parathyroid Hormone in Postmenopausal Women by Chlorella Dietary Supplementation

Kim, Dong-Uk,Seong, Hee-Kyung,Hwang, Jung-Min,Jeon, Ae-Ran,Yun, Ji-Young,Kim, Yong-Ho 대한의생명과학회 2003 Journal of biomedical laboratory sciences Vol.9 No.1

Parathyroid hormone has clearly emerged as the most promising new anabolic treatment for osteoporosis by increasing the activation of osteoblast. It is known that chlorella increases both bone mineral density (BMD) and the rate of bone formation. The purpose of the present study was to determine whether the chlorella dietary supplementation could effect the thyroid or parathyroid hormones associated with increased BMD and bone formation. Twenty-two postmenopausal woman were treated for four month with 4 gm of chlorella dietary supplementation per day, then assessed serum calcium,25 OH vitamin D$_3$, thyroid hormone and parathyroid hormone before and after treatment. The mean 25 OH vitamin D$_3$ and parathyroid hormone were shown to marked increases by 193% and 265% respectively, in contrast to decreases by 9.4%, 37%, 33% and 14% in serum calcium, triiodo-thyroxine, free thyroxine and thyroxine stimulation hormone. In conclusion, treatment of postmenopausal women with chlorella dietary supplementation resulted in an increase in BMD and bone formation through enhancement of parathyroid hormone and 25 OH vitamin D$_3$, and a decrease in thyroid hormones.

²⁷Al and ⁸⁷Rb Nuclear Magnetic Resonance Study of the Relaxation Mechanisms of RbAl(CrO₄)₂·2H₂O Single Crystals

Kim, Jae Sung,Lim, Ae Ran Korean Magnetic Resonance Society 2012 Journal of the Korean Magnetic Resonance Society Vol.16 No.2

The spin-lattice relaxation times, $T_1$, and spin-spin relaxation times, $T_2$, of the $^{27}Al$ and $^{87}Rb$ nuclei in $RbAl(CrO_4)_2{\cdot}2H_2O$ crystals were investigated. The presence of only one resonance line for the $^{27}Al$ nuclei indicates that the results in a dynamical averaging of the crystal electric field that produces a cubic symmetry field. The changes in the temperature dependence of $T_1$ are related to variations in the symmetry of the octahedra of water molecules surrounding $Al^+$ and $Rb^+$. The $T_1$ values for the $^{27}Al$ and $^{87}Rb$ nuclei are different due to differences in the local environments of these ions. We also compared these $^{27}Al$ and $^{87}Rb$ NMR results with those obtained for $RbAl(CrO_4)_2{\cdot}2H_2O$ crystals. The relaxation mechanisms of $RbAl(XO_4)_2{\cdot}nH_2O$ (X=Cr and S) crystals are characterized by completely different NMR behaviors.

Acupuncture Treatment of Adhesive Capsulitis of the Shoulder: A Randomized Controlled Pilot Trial

Kim, Jung-Eun,Kim, Sung-Phil,Kim, Ae-Ran,Park, Hyo-Ju,Kwon, Ojin,Jung, So-Young,Cho, Jung-Hyo,Kim, Joo-Hee,Choi, Sun-Mi Korean AcupunctureMoxibustion Medicine Society 2018 Korean Journal of Acupuncture Vol.35 No.3

Background: Adhesive capsulitis (AC) is a common condition that includes shoulder pain and limited movement. Despite more than 100 years of AC treatment, the most efficacious treatment remains unclear. The aim of this study was to evaluate the feasibility of a randomized controlled trial (RCT) using acupuncture for AC. Methods: Thirty participants with AC were randomly assigned to acupuncture (A) or sham acupuncture (SA) groups. The participants received 15 acupuncture sessions over 6 weeks, and follow-up occurred for an additional 4 weeks thereafter. The primary clinical outcome was the numeric rating scale (NRS) for shoulder pain 6 weeks from the baseline. Secondary outcomes included range of motion (ROM) in the shoulder, the shoulder pain and disability index (SPADI), the EuroQol-5 dimensions (EQ-5D), the Pittsburgh sleep quality index (PSQI), and the patient global impression of change (PGIC). Results: Thirty participants were enrolled out of 37 screened individuals. Recruitment was conducted between August 2014 until May 2015. A total of 28 participants (93%) completed the 6-week intervention, and 26 participants (87%) completed the study. NRS, ROM, SPADI, EQ-5D, PSQI, and PGIC scores improved in both the experimental group and the sham group after 6 weeks, but the difference between the groups was not statistically significant. Adverse events were reported by 12 participants, although these events were not associated with acupuncture. Conclusion: A future RCT for AC may be feasible with some modifications to the recruitment plan and the secondary outcome measurement methods.

Structural Characteristics of 3- and 4-Coordinate Borons from ¹¹B MAS NMR and Single-Crystal NMR in the Nonlinear Optical Material BiB₃O₆

Kim, Woo Young,Lim, Ae Ran Korean Magnetic Resonance Society 2013 Journal of the Korean Magnetic Resonance Society Vol.17 No.1

The structural characteristics of 4-coordinate $BO_4$ [B(1)] and 3-coordinate $BO_3$ [B(2)] groups in $BiB_3O_6$ were studied by $^{11}B$ magic angle spinning (MAS) and single-crystal nuclear magnetic resonance (NMR) spectroscopy. The spin-lattice relaxation time in the laboratory frame, $T_1$, for $^{11}B$ decreased slowly with increasing temperature, whereas the spin-lattice relaxation times in the rotating frame, $T_{1{\rho}}$, for B(1) and B(2), which differed from $T_1$, were nearly constant. Further, $T_{1{\rho}}$ for B(1) and B(2) showed very similar trends, although the $T_{1{\rho}}$ value of B(2) was shorter than that of B(1). The 3-coordinate $BO_3$ and 4-coordinate $BO_4$ were distinguished by $^{11}B$ MAS NMR spectrum and $T_{1{\rho}}$.

Zerp-Degree Excitation Functions of Pion Single Charge Exchange Reactions in Light Nuclei

Kim,Seong-Do,Ji,Ae-Ran,Lee,Mi-Ran,Jyoo,Yeong-Heum 建國大學校基礎科學硏究所 1992 理學論集 Vol.17 No.-

동중 유사 상태로의 파이온 단일전하교환 반응A(π??,π??)A'(lAS)에서 0??~미분 산란 단면적을 30~70Mev 에너지 영역에 걸쳐 계산하였다. 표적으로는 Li?과 C? 핵종을 선택하였으며, 반응이론은 distorted-wave impulse approximation에 의해 기술하였다. 계산에 이용한 광학적포텐셜에서 S-wave 및 P-wave의 효과를 동시에 고려했으며, Pauli-blocking효과도 고려하였다. 0?-미분 산란단면저의 에너지 의존성으로부터 40~50Mev 에너지 영역에서 최소값을 얻었으며,이것은 자유파이온-핵자 단일전하교환 산란단면적의 에너지 의존성에서 볼 수 있는 S-파 와 P-파의 간섭효과를 잘 나타내고 있다. The zero-degree differential cross section calculations for the ?Li(π??,π??)Be?? and ??C(π??,π??)N??reactions to isobaric analog state(IAS) are presented for the energy range between 30∼70 Mev, using the distorted-wave impulse approximation(DWIA)in an eikonal framework. The optical potentials are considered using both S-wave and P-wave, and the Pauli-blocking effect is considered. A deep minimum is found near 40∼50 Mev in the excitation function. It reflects the analogous minima of the interference between S-wave and P-wave which was seen in the free pion-nucleoncleon single charge exchange(SCX)cross section of Rowe et al..

Solution structure of hypothetical protein HP1423 (Y1423_HELPY) reveals the presence of αL motif related to RNA binding

Kim, Ji‐,Hun,Park, Sung Jean,Lee, Ki‐,Young,Son, Woo‐,Sung,Sohn, Na‐,Young,Kwon, Ae‐,Ran,Lee, Bong‐,Jin Wiley Subscription Services, Inc., A Wiley Company 2009 Proteins Vol.75 No.1

Food Science/Microbiology : Characterization of a 27 kDa Fibrinolytic Enzyme from Bacillus amyloliquefaciens CH86-1 Isolated from Cheonggukjang

Ae Ran Lee,Gyoung Min Kim,Jae Yong Park,Hyeon Deok Jo,Jae Ho Cha,Young Sun Song,Ji Yeon Chun,Jeong Hwan Kim 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.1

Bacillus amyloliquefaciens CH86-1 isolated from cheonggukjang was found to have strong fibrinolytic activity when grown on Luria-Bertani medium, and this activity increased sharply when the cells entered the stationary phase. The major fibrinolytic enzyme