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RISS 인기검색어
- 검색키워드
- 저자 : Abo El-Ela Fatma I. (검색결과 2 건)
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Fatma M. M. Korni,Fatma I. Abo El-Ela,Usama K. Moawad 한국어병학회 2023 한국어병학회지 Vol.36 No.1
The current study aimed to identify the primary cause of mortality in a commercial Oreochromis niloticus (O. niloticus) farm. Furthermore, the efficacy of poly-β-hydroxybutyrate (PHB) as a feed additive to prevent mortality was investigated after in-vitro testing. Also, a histopathological examination was carried out. The samples of naturally diseased O. niloticus showed swellings and hemorrhages on the body surface. Moreover, the post-mortem examination revealed black fluids with an awful odor, a congested liver, and intestinal gasses. Concerning diagnosis of the main cause of mortality in diseased O. niloticus, , there were eight isolates might be Edwardsiella tarda based on the biochemical identification results. Also, the identification was confirmed by using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The resulting spectra of two previously identified E. tarda strains were then compared to those found in the Bruker database and the two strains were identified as E. tarda at the species level. For detection of pathogenicity of identified strains, the virulence E. tarda hemolysin (ETHA) gene were detected at band 1078 bp in the eight identified strains. Regarding the in-vitro antimicrobial activity of PHB against E. tarda, the antibacterial activity of blood and tissues had been calculated using the Agar and well diffusion procedures Prior to and after the challenge,. Lower bacterial counts and a larger inhibition zone were signs of the tested materials' concentration-dependent antibacterial activity. For in-vivo evaluation of PHB in dietary-fed O. niloticus, PHB was effective in preventing Edwardsiellosis, with the lowest mortality rates in the group fed 10 g PHB/Kg feed after being injected with E. tarda. In comparison, the group that received a 5 g PHB/Kg feed after receiving an injection of E. tarda saw 16.6% mortalities and a 66.7 percent relative survival rate. The control positive group had 50% mortality. The results of the biochemical testes showed that O. niloticus's kidney and liver functions were unaffected by dietary PHB supplementation. Protein, albumin, globulin, urea, creatinine, aspartate transaminase levels were identical to those of the control negative group. According to the current study, the groups fed high and low PHB concentrations had considerably higher levels of immunoglobulin M, complement 5, and interleukin-1 than the control group. The number of total viable bacteria was lower in the gut of PHB-fed groups than in control. Compared to mild histological alterations in those fed with a lower dosage and severe histopathological abnormalities in the control group, the greater dose of PHB effectively prevented Edwardsiellosis in O. niloticus with decreased mortality and no histopathological changes.
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Ali Shaimaa,Moselhy Walaa A.,Mohamed Hanaa M.,Nabil Taghreed M.,Abo El-Ela Fatma I.,Abdou Kh. 한국독성학회 2022 Toxicological Research Vol.38 No.3
Gibberellic acid (GA3) is a natural plant growth regulator that is crucial for plant structural and functional development. We examined the alleviating capacity of brown algae (Dictyota dichotoma) on biochemical and molecular degenerative processes caused by sub-chronic exposure to gibberellic acid resulting in hepatic cell apoptosis. Adult male albino rats were divided into five equal groups: the first group received distilled water, the second group was treated with GA3, the third group was administered D. dichotoma extract suspended in 1% carboxymethylcellulose (CMC), the fourth group was administered both GA3 and D. dichotoma simultaneously, and the fifth group received 1% CMC orally, 5 days per week for a total of 50 days. The results indicated that GA3 induced a significant increase in liver function parameters based on serum levels of alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and albumin, which indicate hepatotoxicity. A marked increase in malondialdehyde (MDA) levels and a marked decrease in reduced glutathione (GSH), glutathione-S-transferase (GST), and superoxide dismutase (SOD) were observed as a result of induction of lipid peroxidation and oxidative stress. Histopathology revealed severely degenerated hepatocytes including cytoplasmic vacuolations and many apoptotic cells with weak Bcl2 expression. Similarly, there was a significant up-regulation of gene and protein expression levels for the pro-apoptotic markers, Caspase-3 and Bax, and an increase in pro-inflammatory marker levels, tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) as well as C-reactive protein (CRP). The co-administration of D. dichotoma restored the disrupted biochemical, histopathological, molecular, and inflammatory changes resulting from GA3 toxicity. Our results confirm the antioxidant, anti-inflammatory, anti-apoptotic, and hepatoprotective potential of D. dichotoma.
ScienceON
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