Karyotype and RFLP of the nuclear rDNA of Crocidura sp. on Cheju Island, South Korea (Mammalia, Insectivora)

IWASA, M.A.,OHDACHI, S.,HAN, S.-H.,OH, H.-S.,ABE, H.,SUZUKI, H. 濟州大學校 基礎科學硏究所 2003 基礎科學硏究 Vol.16 No.2

We revised the taxonomic status of a white-toothed shrew (Insectivora, Mammalia) on Cheju Island, South Korea, which has been regarded as japanese white-toothed shrew, Crodidura dsinezumi, by analyzing karyotype and restriction fragment length polymorphism (RFLP) for the nuclear ribosoma RNA gene(rDNA) spacer regions. Karyotype of a specimen of Crocidura from Cheju Island was the same as in lesser white-toothed shrew, C. suaveolens, from Tsushima Island and the Eurasian Continent, carrying 2n=40 and FN (fundamental arm number of diploid autosomal set) = 46, whereas C. dsinezumi from the Japanese Islands shows 2n=40 and FN=52. The rDNA-RFLP was examined in two specimens of Crocidura from Cheju Island, two C. dsinezumi from Japan (Honshu and Kyusuh), and one C. suaveolens from the Korean Peninsula, using six restriction sites with the 28S mouse rDNA probe. The shrews from Cheju Islnad possessed four restriction sites common to C. suaveolens from the Koeran Peninsula but only one site common to C. dsinezumi, indicating the shrews from Cheju Island are genetically more close but distinct from the Korean Penisula populations of C. suaveolens. From the results of karyotype and rDNA-RFLP, the specimens from Cheju Island can be regarded as C. suaveolens as a taxonomic unit. Nous avons re´vise´ le statut taxonomique d'une musaraigne a` dents blanched (Mammalia, Insectivora) de l'ile cheju, Core´e du Sud, qui a e´te´ conside´re´e comme la musaraigne japonaise a` dents blanches Crocidura dsinezumi d'apre's I'analyse de son caryotype et de restructuration du polymorphisme d'un fragment de longueur(RFLF) pour les re´gions plus espace´es du ge`ne ribosomal nucle´aire RNA (rDNA). Le caryotype d'un spe´cimen de Crocidura de l'ile Cheju e´tait le me^me qui celui de la petite Crocldura, C. suaveolens, de l'ile Tsushima et du continent eurasien qui a 2n=40 et NF=46, tandis que C. dsinezumi des i^les japonaises a 2n=40 te NF=52. Le rDNA-RFLP a e´te´ examine´ dans deux spe´cimens de Crocidura de l'ile Cheju, deux C. dsinezumi du Japon (Honshu et Kyushu), et un C. suavelens de la pe´ninsule de Core´e, utilisant six sites de restriction avec la sonde rDNA 285 de la souris. Les musaraignes de l'ile Cheju pre´sentent quatre sites de restriction en commun avec C. suaveolens de la pe´ninsule de Core´e, mais seulement un site en commun avec C. dsinezumi, ce qui indique que les musaraignes de l'ile de Cheju sont ge´ne´tiquement plus proches mais distinctes des population de C. suaveolens de la pe´ninsule de core´e. D'apre`s re´sultats du caryotype de RFLP et de rDNA, les spe´cimens de l'ile de Cheju peuvent e^tre regarde´s comme des C. suaveolens.

OGLE-2012-BLG-0563Lb: A SATURN-MASS PLANET AROUND AN M DWARF WITH THE MASS CONSTRAINED BY<i>SUBARU</i>AO IMAGING

Fukui, A.,Gould, A.,Sumi, T.,Bennett, D. P.,Bond, I. A.,Han, C.,Suzuki, D.,Beaulieu, J.-P.,Batista, V.,Udalski, A.,Street, R. A.,Tsapras, Y.,Hundertmark, M.,Abe, F.,Bhattacharya, A.,Freeman, M.,Itow, IOP Publishing 2015 The Astrophysical journal Vol.809 No.1

<P>We report the discovery of a microlensing exoplanet OGLE-2012-BLG-0563Lb with the planet-star mass ratio of similar to 1 x 10(-3). Intensive photometric observations of a high-magnification microlensing event allow us to detect a clear signal of the planet. Although no parallax signal is detected in the light curve, we instead succeed at detecting the flux from the host star in high-resolution JHK'-band images obtained by the Subaru/AO188 and Infrared Camera and Spectrograph instruments, allowing us to constrain the absolute physical parameters of the planetary system. With the help of spectroscopic information about the source star obtained during the high-magnification state by Bensby et al., we find that the lens system is located at 1.3(-0.8)(+0.6) kpc from us, and consists of an M dwarf (0.34(-0.20)(+0.12)M(circle dot)) orbited by a Saturn-mass planet (0.39(-0.23)(+0.14)M(Jup)) at the projected separation of 0.74(-0.42)(+0.26)AU (close model) or 4.3(-2.5)(+1.5)AU (wide model). The probability of contamination in the host star's flux, which would reduce the masses by a factor of up to three, is estimated to be 17%. This possibility can be tested by future high-resolution imaging. We also estimate the (J - K-s) and (H - K-s) colors of the host star, which are marginally consistent with a low metallicity mid-to-early M dwarf, although further observations are required for the metallicity to be conclusive. This is the fifth sub-Jupiter-mass (0.2 < m(p)/M-Jup < 1) microlensing planet around an M dwarf with the mass well constrained. The relatively rich harvest of sub-Jupiters around M dwarfs is contrasted with a possible paucity of similar to 1-2 Jupiter-mass planets around the same type of star, which can be explained by the planetary formation process in the core-accretion scheme.</P>

MOA-2011-BLG-293Lb: A TEST OF PURE SURVEY MICROLENSING PLANET DETECTIONS

Yee, J. C.,Shvartzvald, Y.,Gal-Yam, A.,Bond, I. A.,Udalski, A.,Kozłowski, S.,Han, C.,Gould, A.,Skowron, J.,Suzuki, D.,Abe, F.,Bennett, D. P.,Botzler, C. S.,Chote, P.,Freeman, M.,Fukui, A.,Furusawa, K. IOP Publishing 2012 The Astrophysical journal Vol.755 No.2

<P>Because of the development of large-format, wide-field cameras, microlensing surveys are now able to monitor millions of stars with sufficient cadence to detect planets. These new discoveries will span the full range of significance levels including planetary signals too small to be distinguished from the noise. At present, we do not understand where the threshold is for detecting planets. MOA-2011-BLG-293Lb is the first planet to be published from the new surveys, and it also has substantial follow-up observations. This planet is robustly detected in survey+follow-up data (Delta chi(2) similar to 5400). The planet/host mass ratio is q = (5.3 similar to 0.2) x 10(-3). The best-fit projected separation is s = 0.548 +/- 0.005 Einstein radii. However, due to the s <-> s(-1) degeneracy, projected separations of s-1 are only marginally disfavored at Delta chi(2) = 3. A Bayesian estimate of the host mass gives M-L = 0.43(-0.17)(+0.27) M-circle dot, with a sharp upper limit of M-L < 1.2 M-circle dot from upper limits on the lens flux. Hence, the planet mass is m(p) = 2.4(-0.9)(+1.5) M-Jup, and the physical projected separation is either r(perpendicular to) similar or equal to 1.0 AU or r(perpendicular to) similar or equal to 3.4 AU. We show that survey data alone predict this solution and are able to characterize the planet, but the Delta chi(2) is much smaller (Delta chi(2) similar to 500) than with the follow-up data. The Delta chi(2) for the survey data alone is smaller than for any other securely detected planet. This event suggests a means to probe the detection threshold, by analyzing a large sample of events like MOA-2011-BLG-293, which have both follow-up data and high-cadence survey data, to provide a guide for the interpretation of pure survey microlensing data.</P>

Patient Consent Management by a Purpose-Based Consent Model for Electronic Health Record Based on Blockchain Technology

Dara Tith,Joong-Sun Lee,Hiroyuki Suzuki,W. M. A. B. Wijesundara,Naoko Taira,Takashi Obi,Nagaaki Ohyama 대한의료정보학회 2020 Healthcare Informatics Research Vol.26 No.4

Objectives: Currently, patients’ consent is essential to use their medical records for various purposes; however, most peoplegive their consent using paper forms and have no control over it. Healthcare organizations also have difficulties in dealingwith patient consent. The objective of this research is to develop a system for patients to manage their consent flexibly and forhealthcare organizations to obtain patient consent efficiently for a variety of purposes. Methods: We introduce a new e-consentmodel, which uses a purpose-based access control scheme; it is implemented by a blockchain system using HyperledgerFabric. All metadata of patient records, consents, and data access are written immutably on the blockchain and shared amongparticipant organizations. We also created a blockchain chaincode that performs business logic managing patient consent. Results: We developed a prototype and checked business logics with the chaincode by validating doctors’ data access withpurpose-based consent of patients stored in the blockchain. The results demonstrate that our system provides a fine-grainedway of handling medical staff ’s access requests with diverse intended purposes for accessing data. In addition, patients cancreate, update, and withdraw their consents in the blockchain. Conclusions: Our consent model is a solution for consentmanagement both for patients and healthcare organizations. Our system, as a blockchain-based solution that provides highreliability and availability with transparency and traceability, is expected to be used not only for patient data sharing in hospitals,but also for data donation for biobank research purposes.

REVISITING THE MICROLENSING EVENT OGLE 2012-BLG-0026: A SOLAR MASS STAR WITH TWO COLD GIANT PLANETS

Beaulieu, J.-P.,Bennett, D. P.,Batista, V.,Fukui, A.,Marquette, J.-B.,Brillant, S.,Cole, A. A.,Rogers, L. A.,Sumi, T.,Abe, F.,Bhattacharya, A.,Koshimoto, N.,Suzuki, D.,Tristram, P. J.,Han, C.,Gould, A American Astronomical Society 2016 The Astrophysical Journal Vol.824 No.2

<P>Two cold gas giant planets orbiting a G-type main-sequence star in the galactic disk were previously discovered in the high-magnification microlensing event OGLE-2012-BLG-0026. Here, we present revised host star flux measurements and a refined model for the two-planet system using additional light curve data. We performed high angular resolution adaptive optics imaging with the Keck and Subaru telescopes at two epochs while the source star was still amplified. We detected the lens flux, H = 16.39 +/- 0.08. The lens, a disk star, is brighter than predicted from the modeling in the original study. We revisited the light curve modeling using additional photometric data from the B&C telescope in New Zealand and CTIO 1.3 m H-band light curve. We then include the Keck and Subaru adaptive optic observation constraints. The system is composed of a similar to 4-9 Gyr lens star of M-lens = 1.06 +/- 0.05 M circle dot at a distance of D-lens = 4.0 +/- 0.3 kpc, orbited by two giant planets of 0.145 +/- 0.008 M-Jup and 0.86 +/- 0.06 M-Jup, with projected separations of 4.0 +/- 0.5 au and 4.8 +/- 0.7 au, respectively. Because the lens is brighter than the source star by 16 +/- 8% in H, with no other blend within one arcsec, it will be possible to estimate its metallicity using subsequent IR spectroscopy with 8-10 m class telescopes. By adding a constraint on the metallicity it will be possible to refine the age of the system.</P>

Application of Blockchain to Maintaining Patient Records in Electronic Health Record for Enhanced Privacy, Scalability, and Availability

Dara Tith,Joong-Sun Lee,Hiroyuki Suzuki,W. M. A. B. Wijesundara,Naoko Taira,Takashi Obi,Nagaaki Ohyama 대한의료정보학회 2020 Healthcare Informatics Research Vol.26 No.1

Objectives: Electronic Health Record (EHR) systems are increasingly used as an effective method to share patients’ records among different hospitals. However, it is still a challenge to access scattered patient data through multiple EHRs. Our goal is to build a system to access patient records easily among EHRs without relying on a centralized supervisory system. Methods: We apply consortium blockchain to compose a distributed system using Hyperledger Fabric incorporating existent EHRs. Peer nodes hold the same ledger on which the address of a patient record in an EHR is written. Individual patients are identified by unique certificates issued by a local certificate authorities that collaborate with each other in a channel of the network. To protect a patient’s privacy, we use a proxy re-encryption scheme when the data are transferred. We designed and implemented various chaincodes to handle business logic agreed by member organizations of the network. Results: We developed a prototype system to implement our concept and tested its performance including chaincode logic. The results demonstrated that our system can be used by doctors to find patient’s records and verify patient’s consent on access to the data. Patients also can seamlessly receive their past records from other hospitals. The access log is stored transparently and immutably in the ledger that is used for auditing purpose. Conclusions: Our system is feasible and flexible with scalability and availability in adapting to existing EHRs for strengthening security and privacy in managing patient records. Our research is expected to provide an effective method to integrate dispersed patient records among medical institutions.

A Likely Detection of a Two-planet System in a Low-magnification Microlensing Event

Suzuki, D.,Bennett, D. P.,Udalski, A.,Bond, I. A.,Sumi, T.,Han, C.,Kim, Ho-il.,Abe, F.,Asakura, Y.,Barry, R. K.,Bhattacharya, A.,Donachie, M.,Freeman, M.,Fukui, A.,Hirao, Y.,Itow, Y.,Koshimoto, N.,Li, American Astronomical Society 2018 The Astronomical journal Vol.155 No.6

A Bayesian cure rate model with dispersion induced by discrete frailty

Cancho, Vicente G.,Zavaleta, Katherine E.C.,Macera, Marcia A.C.,Suzuki, Adriano K.,Louzada, Francisco The Korean Statistical Society 2018 Communications for statistical applications and me Vol.25 No.5

In this paper, we propose extending proportional hazards frailty models to allow a discrete distribution for the frailty variable. Having zero frailty can be interpreted as being immune or cured. Thus, we develop a new survival model induced by discrete frailty with zero-inflated power series distribution, which can account for overdispersion. This proposal also allows for a realistic description of non-risk individuals, since individuals cured due to intrinsic factors (immunes) are modeled by a deterministic fraction of zero-risk while those cured due to an intervention are modeled by a random fraction. We put the proposed model in a Bayesian framework and use a Markov chain Monte Carlo algorithm for the computation of posterior distribution. A simulation study is conducted to assess the proposed model and the computation algorithm. We also discuss model selection based on pseudo-Bayes factors as well as developing case influence diagnostics for the joint posterior distribution through ${\psi}-divergence$ measures. The motivating cutaneous melanoma data is analyzed for illustration purposes.

Simulation of a Polarimeter for a Spin - Polarized Positron Beam

J. H. Kim,F. Saito,N. Suzuki,L. Wei,Y. Nagashima,T. Kurihara,A. Goto,Y. Itoh,Y. S. Lee,T. Hyodo 한국진공학회(ASCT) 2002 Journal of Korean Vacuum Science & Technology Vol.6 No.3

A performance of a new positron polarimeter is investigated by simulation using a charged-particle trajectory program. The results of the ray tracing are presented along with the details of the design parameters and projected system performance. A ray tracing analysis indicates that this design is capable of effectively transmitting positrons at beam energies varying from 0.1 to 30 keV within the beam diameter of 2-6 ㎜. However, the observed reflection of the positrons(1ower than 2 keV) at 12 kGauss indicated that further refinement of beam design is needed to produce a better positron polarimeter.

Cytokeratin 13, Cytokeratin 17, and Ki-67 Expression in Human Acquired Cholesteatoma and Their Correlation With Its Destructive Capacity

Mahmood A. Hamed,Seiichi Nakata,Kazuya Shiogama,Kenji Suzuki,Ramadan H. Sayed,Yoichi Nishimura,Noboru Iwata,Kouhei Sakurai,Badawy S. Badawy,Ken-ichi Inada,Hayato Tsuge,Yutaka Tsutsumi 대한이비인후과학회 2017 Clinical and Experimental Otorhinolaryngology Vol.10 No.3

Objectives. Cholesteatoma is a nonneoplastic destructive lesion of the temporal bone with debated pathogenesis and bone resorptive mechanism. Both molecular and cellular events chiefly master its activity. Continued research is necessary to clarify factors related to its aggressiveness. We aimed to investigate the expression of Ki-67, cytokeratin 13 (CK13) and cytokeratin 17 (CK17) in acquired nonrecurrent human cholesteatoma and correlate them with its bone destructive capacity. Methods. A prospective quantitative immunohistochemical study was carried out using fresh acquired cholesteatoma tissues (n=19), collected during cholesteatoma surgery. Deep meatal skin tissues from the same patients were used as control (n=8). Cholesteatoma patients were divided into 2 groups and compared (invasive and noninvasive) according to a grading score for bone resorption based upon clinical, radiologic and intraoperative findings. To our knowledge, the role of CK17 in cholesteatoma aggressiveness was first investigated in this paper. Results. Both Ki-67 and CK17 were significantly overexpressed in cholesteatoma than control tissues (P<0.001 for both Ki-67 and CK17). In addition, Ki-67 and CK17 were significantly higher in the invasive group than noninvasive group of cholesteatoma (P=0.029, P=0.033, respectively). Furthermore, Ki-67 and CK17 showed a moderate positive correlation with bone erosion scores (r=0.547, P=0.015 and r=0.588, P=0.008, respectively). In terms of CK13, no significant difference was found between cholesteatoma and skin (P=0.766). Conclusion. Both Ki-67 and CK17 were overexpressed in cholesteatoma tissue and positively correlated with bone resorption activity. The concept that Ki-67 can be a predictor for aggressiveness of cholesteatoma was supported. In addition, this is the first study demonstrating CK17 as a favoring marker in the aggressiveness of acquired cholesteatoma.