인플루엔자 바이러스 검출을 위한 종이 기반 neuraminidase 효소 활성 평가 센서 개발

황철환 ( Cheol-hwan Hwang ),정성근 ( Seong-geun Jeong ),박한규 ( Han-kyu Park ),이창수 ( Chang-soo Lee ),김윤곤 ( Yun-gon Kim ) 한국화학공학회 2016 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.54 No.3

In this study, we described a paper-based neuraminidase assay sensor (PNAS) which can be applied to detect the infection by influenza viruses. The PNAS was designed and manufactured to quantitatively identify the levels of neuraminidase in the sample, which is based on colorimetric analysis using the X-Neu5Ac substrate. The limit of detection of the PNAS was determined as 0.004 U/mL of neuraminidase. According to the amount of neuraminidase in human serum, the PNAS could monitor the enzyme activity with a good linearity (R2 > 0.99). In addition, the initial performance of the PNAS has been maintained up to 70 days in the 4 ℃. Finally, we demonstrated whether the Michaelis- Menten kinetics is applied to the PNAS, which can show the reliability of the enzyme reactions. The kinetic studies indicated that the PNAS provides the good condition for enzyme reactions (Km=8.327×10.3 M), but they were performed on paper chip nonetheless. The paper-based neuraminidase assay sensor may be useful in a wide range of rapid and safe detection of influenza virus.

디지털이미지를 이용한 모션픽처 교육에 관한 연구

황철환 ( Cheol Hwan Hwang ) 한국사진학회 2012 AURA Vol.0 No.28

질량분석 시스템을 이용한 극지 토양 유래 신규 미생물의 지질 A 화학적 구조 분석

황철환(Cheol-hwan Hwang),박한규(Han-Gyu Park),김윤곤(Yun-Gon Kim) 한국생물공학회 2016 KSBB Journal Vol.31 No.4

For decades, the microorganisms in arctic soils have been newly discovered according to the climate change and global warming. In this study, the chemical structure of a lipid A molecule from Pseudomonas sp. strain PAMC 28615 which was newly discovered from arctic soils was characterized by mass spectrometric approaches such as matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and MALDI multi-stage tandem mass spectrometry (MS). First, lipopolysaccharide (LPS) from Pseudomonas sp. strain PAMC 28615 was extracted and subsequently hydrolyzed to obtain the lipid A. The parent ion peak at m/z 1632 was determined by MALDI-TOF MS, which also can validate our lipid A purification method. For detailed structural determination, we performed the multiple-stage tandem mass analysis (MS<SUP>4</SUP>) of the parent ion, and subsequently the abundant fragment ions in each MS stage are tested. The fragment ions in each MS stage were produced from the loss of phosphate groups and fatty acyl groups, which could be used to confirm the composition or the position of the lipid A components. Consequently, the mass spectrometry-based lipid A profiling method could provide the detail chemical structure of lipid A from the Pseudomonas sp. strain PAMC 28615 as an arctic bacterium from the frozen arctic soil.

보리에 대한 제초체 Propisochlor 약해의 변동요인 및 경감효과

황철환(Cheol-Hwan Hwang),노석초(Seog-Cho Lo),마상용(Sang-Yong Ma),한성수(Seong-Soo Han) 韓國雜草學會 2006 Weed&Turfgrass Science Vol.26 No.2

작품 : 어둠(밤)에 관한 시각화와 디지털 이미지의 활용

황철환 ( Cheol Hwan Hwang ) 한국사진학회 2006 AURA Vol.0 No.14

타이포그래피와 디지털이미지의 조형 연구

황철환 ( Cheol Hwan Hwang ) 한국사진학회 2010 AURA Vol.0 No.23

Text and image have been in competitive relation to each other as a way to express human emotion, showing their actual existences in history. After Renaissance, Text having taken more steps from simply explaining Image has been developed as a variety of means to describe over the collage of the text cubism and futurism and the language experiment like dadaism and surrealism as well as the concept art. Text which is a sentence of "The images flooded the era of the digital" comes into contact as much. Now the text which was the old way of transferring communication into images of the development of a variety and emerging visual media with communicating a clear and strong message. However, the text is not disappearing. Text becomes image, and the combination of text and image is found easier in everyday life. Moreover, the emotion in the era of the digital is the text, which is the image. The text plays an important role in the relationship to languages which are exchanging between the human characters. Initially, the symbol has been created from text in the changes of history. The birth of today`s many kind of texts has an important position for the preservation of our culture. In particular, the role of modern text is more important in that it refers to the communication for us as well as fun and beauty of aesthetic art in our live. Therefore, the basic starting point for this paper research is on the character of the text and the meaning of the text use in the painting representations of modern arts. In other words, this study researches the expression of visual art with text, as well as meaning of the text in the modern arts. In addition to that, this study tries to add from geometric of text the basic geometric of the object of Paul Cezanne who analyzed about the object with "sphere, cone, cylinder". Text of the character in the image gives a sign of the communication, and the autonomous and communication of sing. Image representation in the humans five senses has been in relation to the visual, but it can be concluded that another viewpoint of sing and arts will be needed. Thanks.

시대성이 반영된 원자력발전에 대한 일반인들의 인식 분석

박철구(Cheol Koo Park),황철환(Chul Hwan Hwang),김동현(Dong Hyun Kim) 한국방사선학회 2017 한국방사선학회 논문지 Vol.11 No.6

본 연구는 일반인을 대상으로 시대성이 반영된 각종 잠재적 위험요소와 원자력(방사선)발전의 위험성 대한 인식을 분석하였다. 설문 대상은 다양한 계층으로 하고 그 중에 총 293부를 분석하였다. 그 결과, 잠재적 위험요소 중에서 화재에 대한 위험도 인식이 높게 나타났으며, 다음으로 방사선 테러와 핵(원자력)에너 지의 위험도를 다른 위험요소들에 비해 비교적 높게 인식하였다. 연령별, 학력별, 정치 이념 성향에 따른 분석에서 원자력발전의 필요성과 위험성, 안전성에 대해서 상반된 결과를 나타내었다. 정치이념의 성향에 따른 잠재적 위험요소와 원자력발전에 대한 인식은 보수적 이념집단에서 긍정적인 인식을, 진보적 집단에 서는 부정적인 인식을 하고 있는 것으로 나타났다. 즉, 정치 이념의 성향에 따라 원자력발전 인식 분석에서 통계적 차이를 보였다. 따라서 원자력(방사선)발전 정책 방향 설정과 방사선 이용 관련 산업에 있어서 전문가 의견과 일반인의 다양한 의견을 반영해서 결정되어져야 할 것으로 판단되고, 일반인도 사실을 바탕으로 객관적이고 과학적인 근거에 입각해 각종 잠재적 위험과 원자력(방사선)에 대해 막연한 불안감을 가지지 말고 유연한 대처를 할 필요가 있다고 판단된다 This study investigates the perception of the general public through analysis of various potential risk factors reflecting nuclear power generation and nuclear power (radiation) and risks. A total of 293 copies of the data were collected from various strata in Busan. As a result, among the potential factors in everyday life, there was a high awareness of the risk to the fire. Next, the perception of risk for radiation terror and nuclear (nuclear) energy was relatively high compared to other risk factors. In the analysis according to age, educational background, and political ideological tendency, the results were contradictory to the necessity, risk and safety of nuclear power generation. The potential risk factors and the perception of nuclear power according to the tendency of political ideology were analyzed to be positive recognition of conservative ideology and negative recognition of progressive group. In other words, the perception of nuclear power was analyzed differently according to the tendency of political ideology. Therefore, it should be decided to reflect the opinions of experts and various opinions of the general public in the setting of nuclear radiation (radiation), it is believed that ordinary people need to take flexible action without having a vague sense of anxiety about various potential risks and nuclear power (radiation) based on objective and scientific grounds.

두경부환자의 토모테라피 치료시 SETUP 변화율에 대한 후향적 평가

하태영,김승준,황철환,손종기,Ha, Tae-young,Kim, Seung-jun,Hwang, Cheol-hwan,Son, Jong-gi 대한방사선치료학회 2016 대한방사선치료학회지 Vol.28 No.1

목 적 : 두경부 환자 토모 치료 시 위치 보정한 좌표값을 이용한 환자의 Setup 변화율을 후향적 평가를 하고자 한다. 재료 및 방법 : 2010년 01월에서 2012년12월까지 토모테라피 치료를 받은 두경부 환자 중 무작위(95)로 샘플링하여 3그룹으로 정리하였다. 그룹 1(32)Brain, 그룹2(28)Maxillar, Nasal cavity, 그룹3(35) Nosopharynx(NPX), Tongue, Tonsil, Oropharynx(OPX)로 분류하였다. 3그룹 간에 있어 X 축, Y 축, Z 축 오차, Roll, 체중변화, Vector를 변수로 하여, 30회 치료기간 중 반복측정에 의한 통계적 검정을 시행하였다. 결 과 : 통계적 검정 결과 fraction에 따른 차이는 x축(p=0.458), y축(0.989)은 차이가 없었으며 z축(p=0.001), roll(p=0.037), 체중변화(p<0.001), Vector(p<0.001)은 차이가 있는 것으로 나타났다. 또한 fraction에 따라 3그룹간의 패턴은 x축(p=0.430), roll(p=0.299)은 차이가 없었으며 y축(0.023), y축(0.023), 체중변화(p=0.001), Vector(p=0.028)은 차이가 있는 것으로 나타났다. 결 론 : 후향적 평가를 시행한 결과 그룹3즉, Y, Z, 체중변화, Vector 변화에 대하여 알 수 있었고 Low neck을 포함한 치료 시 Random error가 커짐을 통계적 알 수 있었다. Purpose : Retrospective evaluation of setup changes using the corrected position during helical tomotherapy Materials and Methods : Head and neck cancer patients were randomly sampled and summarized into 3 groups: Group 1(32) Brain, Group 2 2(28)Maxillar, Nasal cavity, Group 3 (35) Nasopharynx(NPX), Tongue, Tonsil, and Oropharynx(OPX). In 3 groups, the statistical tests based on repeated measurements among 30 times of the duration of treatment by applying X, Y, Z axis errors, roll, weight changes, and vectors as variables. Results : The statistical test results showed that there was no difference between x-axis (p = 0.458) and y-axis (p=0.986) and in roll (p = 0.037), weight change (p <0.001), and the vector (p <0.001). In addition, the pattern between the three groups based on the fraction revealed no difference in x-axis (p = 0.430) and roll (p = 0.299) but a difference in y-axis (.023), weight change (p = 0.001), and vector (p = 0.028). Conclusion : The results of the retrospective evaluation found the change in the group 3 with respect Y, Z, weight, and vector and a larger random error during the treatment including low neck.

살충제 thiodicarb의 단감 중 잔류분석 및 안전성 평가

노석초(Seog Cho Lo),황철환(Cheol Hwan Hwang),김민수(Min Soo Kim),마상용(Sang Yong Ma),한성수(Seong Soo Han) 한국농약과학회 2004 농약과학회지 Vol.8 No.3

The residue analysis of the insecticide thiodicarb in sweet persimmon was analyzed with a gas chromatograph equipped with nitrogen phosphorus detector(NPD) to evaluate efficacy of analysis method and safety of thiodicarb in persimmon. Minimum detectable amount of methomyl oxime, hydrolysate of thiodicarb, was 0.2 ng and detection limit of thiodicarb was 0.05 ppm. The mean recoveries evaluated from untreated samples spiked at 0.5 ppm and 2.5 ppm were 93.9±3.9 and 92.8±2.0%, respectively. When thiodicarb was sprayed onto the sweet persimmon two times until 40 and 30 days before harvest, three times until 40, 30 and 21 days before harvest, and four times until 40, 30, 21 and 14 days before harvesting, the mean residual amount in sweet persimmon were 1.50±0.04, 1.86±0.04 and 2.11±0.12 ppm, respectively. It would be safe when thiodicarb 40% WP is treated four times 14 days before harvesting to control fruit moth in sweet persimmon.

노출량에 따른 디지털사진과 은염사진의 화질 비교

김지홍 ( Jee Hong Kim ),황철환 ( Cheol Hwan Hwang ) 한국사진학회 2007 AURA Vol.0 No.17