Monochloroacetaldehyde의 합성에 관한 연구

홍성엽 서경대학교 산업기술연구소 2000 産業技術硏究所論文集 Vol.8 No.-

Monochloroacetaldehyde is a very useful compound as a raw material for the synthesis of medicinal and agricultural chemicals. Because of the unstability of the monochloroacetaldehyde, many new synthetic method for the compound were proposed such as the chloroacetaldehyde's concentration in water and chloroacetaldehyde trimer. In this report, some new factors were proposed to improve the yield to synthesize and the purity of monochloroacetaldehyde. The synthetic method of monochloroacetaldehyde in organic solvent instead of in water have shown good result in the point of the yield and the purity.

Designed Urease의 합성

홍성엽 서경대학교 산업기술연구소 2007 産業技術硏究所論文集 Vol.19 No.-

Urease is a enzyme which catalyzes the urea hydrolysis. The design urease is mimiced the urease. The structure of the designed urease is composed of macrocyle and Zn. The macrocycle compound is synthesized by the reaction between picolyl chloride and tetraazatetradecane. The designed urease is accomplished by the chelation of Zn2+ ion to the macrocycle compound.

고안된 urease의 디자인 및 합성

홍성엽 서경대학교 산업기술연구소 2008 産業技術硏究所論文集 Vol.21 No.-

Urease is a enzyme to hydrolyze urea to ammonia and carbamate. In this article, simplified urease was designed and synthesized. The synthesis of the urease was obtained by reaction of picolyl chloride and 1,4,8,11-tetraazacyclotetradecane at near 0 oC.

Biodiesel 생산 과정의 부산물인 Glycerin의 활용

홍성엽 서경대학교 산업기술연구소 2010 産業技術硏究所論文集 Vol.24 No.-

Biodiesel is produced by transesterification reaction between vegetable oil and methanol with catalysts Glycerin is obtained from the transesterification reaction as a by-product. In this research, a glycerin ester and glycerin ether as derivatives of the glycerin were synthesized. The glycerin ester was obtained esterification reaction between glycerin and acetic anhydride.

거대 고리 화합물의 새로운 합성 방법

홍성엽 서경대학교 산업기술연구소 2007 産業技術硏究所論文集 Vol.19 No.-

Macrocyclic compounds are very useful for various biological application. The various synthetic methods are known for the macrocyclic compounds. In this article, the new synthetic method is proposed to obtain 1,4,8,11-tetraazatetradecane. 1,3-Diaminopropane is used as a starting compound to obtain the cyclic compound. After cyclic amide was obtained, LiAlH4 was used to obtain the target compound by reduction.

The Current Status and Future Outlook of Quantum Dot-Based Biosensors for Plant Virus Detection

홍성엽,이철호 한국식물병리학회 2018 Plant Pathology Journal Vol.34 No.2

Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), widely used for the detection of plant viruses, are not easily performed, resulting in a demand for an innovative and more efficient diagnostic method. This paper summarizes the characteristics and research trends of biosensors focusing on the physicochemical properties of both interface elements and bioconjugates. In particular, the topological and photophysical properties of quantum dots (QDs) are discussed, along with QD-based biosensors and their practical applications. The QD-based Fluorescence Resonance Energy Transfer (FRET) genosensor, most widely used in the biomolecule detection fields, and QD-based nanosensor for Rev-RRE interaction assay are presented as examples. In recent years, QDbased biosensors have emerged as a new class of sensor and are expected to open opportunities in plant virus detection, but as yet there have been very few practical applications (Table 3). In this article, the details of those cases and their significance for the future of plant virus detection will be discussed.

Quantitative lateral-flow immunoassay for the assessment of the cartilage oligomeric matrix protein as a marker of osteoarthritis

홍성엽,박유민,장요한,민병현,윤현철 한국바이오칩학회 2012 BioChip Journal Vol.6 No.3

This study reports a simple and quantita-tive analytical method for detecting cartilage oligome-ric matrix protein(COMP) using a lateral-flow immu-noassay(LFI) format. An immuno-chromatographic assay was developed using a gold nanoparticle(AuNP)-conjugated monoclonal antibody(Ab) probe for the quantitative detection of COMP in human synovial fluid(SF). The detection antibody was conjugated with the AuNP(~30nm) to enable detection, and the cap-ture antibody was immobilized within a multi-spot pattern in the test zone of a membrane strip. COMP in the human SF binds to the AuNP-conjugated detection antibody, and the COMP-Ab complex then binds to the capture antibody. The immunoassay test can be analyzed by visualizing the test strip with a commer-cial scanner and an imaging software program. The color density of the multi-spot pattern at the test zone increased in proportion to the concentration of COMP. The developed immunoassay exhibited a dynamic de-tection range from 0.6 to 20μg/mL of COMP. The detection limit of the proposed LFI system was approx-imately 0.2μg/mL. The test results showed good cor-relation with those obtained using a conventional en-zyme-linked immunosorbent assay(ELISA) to detect COMP. We expect that the proposed immunoassay method, based on the quantification of COMP, is sui-table for osteoarthritis diagnosis using SF samples.

A Study on Carbon Fiber Reinforced Plastic Focusing on the Automotive Applications

홍성엽 한국전시산업융합연구원 2019 한국과학예술융합학회 Vol.37 No.5

Carbon fiber reinforced polymer (CFRP) is used in the various areas included automobiles. The matrix used in fiber reinforced polymer is the resin that interacts with the various elements of FRP and is generally classified into thermosetting polymers and thermoplastic polymers. Generally, thermosetting polymers were used a lot. Carbon fiber reinforced polymer is a high-strength, high-elastic material and lightweight material, which is used as a core material to manufacture for structural materials of cars. Productivity in the mass-production automotive industry should be significantly faster than the conventional composite material molding method. Carbon fiber reinforced thermoplastic polymer (CFRTP) and additive manufacturing may be alternatives as a material and molding method, respectively. CFRTP is a low-cost fabrication material that has received much attention in recent years due to its properties of high elastic modulus, high strength, and light weight. Additive manufacturing is expected to solve the costly traditional processes in that it can produce polymeric composite materials in an inexpensive and efficient manner. The research method is as follows. Firstly, this paper is an overview of the application of CFRP to automobiles. The recent technological achievements for the CFRP were review and reorganized to focus for the possible alternatives of CFRP. Various domestic and foreign electronic journals, such as Science Direct and Springer Link, were used to collect data, and the Internet and Internet sites of automobile companies to collect various data on the current state of the automobile industry. Secondly, the purpose of this article is to review and redefine the advantages and disadvantages of CFRPs, carbon fiber reinforced thermosetting polymer using thermosetting polymer and carbon fiber reinforced thermoplastic polymer using thermoplastic polymers, improvement direction of CFRP, process alternatives and others. Finally, this research proposes carbon fiber reinforced thermoplastic polymer (CFRTP) is acceptable alternate material to carbon fiber reinforced polymer and Additive manufacturing is possible technology to solve the costly traditional processes.

급성흉통의 진단적 프로토콜에 대한 전향적 연구

홍성엽,김갑득,강명희 대한응급의학회 2003 대한응급의학회지 Vol.14 No.3

Purpose: For appropriate triage of chest pain patients in the emergency room (ER), We verified whether or not a new chest pain protocol, which is a combination of Goldman algorithm Cardiac STATus® adequately reflects the risk of a major cardiac event in patients with acute chest pain. Methods: All patients arriving at the ER were divided into 4 risk group based on the Goldman Ml probability and on the result of cardiac STATus®. one month later, we collected information on whether the patients had undergone major cardiac event or death. We analyzed relative risk (RR) of cardiac event by using a logistic regression and we com pare the event-free survival rates and survival lengths. RR=2 mean that the risk of cardiac events is high by two folds than control group. Results: There were 31 major cardiac events. the relative risk of major events of each group compared to group 4 were 40.1 for group 1, 51.9 for group 2 and 27.4 for group 3. Group 4 patients experienced one cardiac event and no deaths. The survival time without a cardiac event of group 4 patients approach 30 days within experimental error. The survival rate without a cardiac event is 0.98±0.02. Therefore, the patients in group 4 had life expectancies similar to those of normal persons. Conclusion: This chest pain protocol adequately reflects the chances of cardiac event, so patients who show a negative result in cardiac STATus® and a low Goldman MI probability can be safely discharged from ER and followed up in outpatient clinic.

시부트라민의 합성 및 분석 방법에 대한 연구

홍성엽 서경대학교 산업기술연구소 2010 産業技術硏究所論文集 Vol.25 No.-

Sibutramine is originally known as an anti-obesity drug. The biological activities of sibutramine are various, such as depression, parkinson`s disease, diabetes mellitus, and obesity. Sibutramine maleate was synthesized from sibutramine free base which also synthesized by 4 steps reactions. The intermediates to synthesize sibutramine are 1-(4-chlorophenyl)-1-cycloisobutylcarbonitrile, 1-isobutyryl-1-(4-chlorophenyl)cyclobutane, and N-formyl-1-[1-(4- chlorophenyl)cyclobutyl]isobutylamine. The synthesized sibutramine maleaste are assayed by UV/visible spectroscopy and HPLC with UV/visible detector. The purity of the synthesized sibutramine maleate are determined from the HPLC data.