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파라핀 기반의 조직회수도구를 사용한 채취 조직의 진단 프로토콜 개발
정효영,구교인,이상민,박호수,홍석준,방승민,송시영,조동일,Jeong, Hyo-Young,Koo, Kyo-In,Lee, Sang-Min,Park, Ho-Soo,Hong, Suk-Jun,Bang, Seoung-Min,Song, Si-Young,Cho, Dong-Il 대한의용생체공학회 2010 의공학회지 Vol.31 No.3
We have developed and reported several micro-spikes for minimally invasive biopsy. This paper presents a diagnosis protocol for micro-spike biopsy using paraffin-based tissue collecting tool. Using the proposed tissue collecting tool, which has a negative micro-spike structure in a porous chamber, the biopsied tissue in a micro-spike is effectively detached. The proposed diagnosis protocol prevents the loss of tissues in a paraffin embedding and sectioning process. Hence, it is compatible with conventional histopathology without additional reagents and processes. The gastro-intestinal tissue of a pig is biopsied in an in vivo environment, and then it is detached from a micro-spike using the paraffin-based tissue collecting tool. A histopathological photomicrograph of the detached tissue is acquired with the proposed diagnosis protocol. The acquired image offers clinical quality. This result shows that the paraffin-based tissue collecting tool is applicable to the medical practice.