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전재우(Jae Woo Jun),박소희(So Hee Park),김수향(Su-Hyang Kim),지경수(Kyoung Soo Jee),이시우(Shi Woo Lee),신지원(Ji Won Shin),신정욱(Jung-Woog Shin) 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5
The bone cells continuously experience mechanical stimuli during the daily activities. In this study, through the co-culturing system the effects of hydrostatic pressure on osteoprecursor cells were evaluated. M2-10B4(Murine stromal cell) and RAW264.7(Murine osteoclast precursor) were plated were placed on 6-well plate and on a transwell insert with 5×10⁴ cells each. The both cells were exposed daily to a hydrostatic pressure of 0.1 ㎫ or 0.2 ㎫ for 2 min followed by 15 min rest for 4 hours per day by a bioreactor. The M2-10B4s proliferated less when they were co-cultured. However, the highest ALP activity when normalized by DNA contents was found in co-culturing with 0,.2 ㎫ stimulation(RMS0.2).