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바이오에탄올 생산을 위한 폐MDF의 전처리 및 효소 당화
강양래(Yang-Rae Kang),황진식(Jin-Sik Hwang),배기한(Ki-Han Bae),조훈호(Hoon-Ho Cho),이은정(Eun-Jeong Lee),조영손(Young-Son Cho),남기두(Ki-Du Nam) 한국생물공학회 2015 KSBB Journal Vol.30 No.6
The objective of this study was designed to determine the possibility of bioethanol production from wasted medium density fiberboard (wMDF). We were investigated the enzymatic saccharification characteristics using the enzyme (Cellic CTec3) after pretreatment with sodium chlorite. According to the component analysis results, the lignin contents before and after the pretreatment of wMDF (milling using sieve size of 1,000 μm) was significantly reduced from 31.13% to 4.11%. Therefore, delignification ratio of pretreated wMDF was found to be up to about 87-89% depending on the sieve size. And we were tested to compare the saccharification ratio according to the sieve size of wMDF (1,000 μm, 200 μm), but it was no significance depending on the sieve size. When enzyme dosage was 5% based on the substrate concentration, enzymatic saccharification ratio was obtained up to 70% by maintaining at 50℃ for 72 hours. We could made the substrate concentration of pretreated wMDF (1,000 μm) up to 12% and then enzymatic saccharification ratio was 76.8%, also contents of glucose and xylose were analyzed to 77,750 and 14,637 mg/L, respectively.
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강양래(Yang-Rae Kang),황진식(Jin-Sik Hwang),배기한(Ki-Han Bae),조훈호(Hoon-Ho Cho),이은정(Eun-Jeong Lee),조영손(Young-Son Cho),남기두(Ki-Du Nam) 한국생물공학회 2016 KSBB Journal Vol.31 No.1
The aim of this study attempted to verify the possibility of bioethanol production using wasted medium density fiberboard (wMDF). In order to produce bioethanol from wood cellulosic materials must be carried out the process of pretreatment, saccharification, fermentation and distillation. First, the wMDF was pretreated using sodium chlorite and pretreated wMDF was prepared to 8% slurry and then slurry was saccharified with the commercial enzyme (Cellic CTec3). The fermentable sugar and pH of saccharified substrate were about 5.5% glucose and 4.4, respectively. Herein we compared the results of ethanol yield according to the nutrients added or without addition to increase ethanol yield. Ethanol fermentation was finished in about 24 hours, but it was delayed in experimental group without nutrients. Ethanol content and fermentation ratio of the final fermented mash prepared by utilizing jar fermenter was 25.40 g/L and 86.64%, respectively. At this time, the maximum ethanol productivity was confirmed as 1.78 g/Lh (ethanol content 21.38 g/L, 12 h), and the overall ethanol productivity was 1.05 g/Lh (ethanol content 25.27 g/L, 24 h). Using fermented liquid we could produced bioethanol 95.37% by continuous distillator packed with copper element in laboratory scale. These results show that wMDF has a potential valuable for bioethanol production.
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남기두,이인기,조훈호 한국산업미생물학회 1991 한국미생물·생명공학회지 Vol.19 No.5
효모 재순환계에 의한 당밀의 연속발효를 하였다. 단계적으로 YPD배지 농도를 10g/l에서 183.5g/l로 증가시킨 후 최종 발효배지는 당밀로 하였다. 최고의 효모농도는 25g/l였고, 운전중 평균 효모농도는 23.5g/l으로 종래 회분식보다 3.4배 높았다. 최적 발효조건은 180g/l의 당밀배지를 공급하였을 때 교반속도 500rpm, 희석율 0.037h^-1였다. 이 조건을 645시간 연속 운전하였으며 최고 알코올 생산성은 4.9g/l·h로서 이때의 희석율은 0.091h^-1, 알코올 농도는 53.9g/l이었다. In this study continuous alcohol fermentation of molasses by the recirculation system has investigated. After cultivation of yeast cells in the YPD medium with increasing the medium concentration from 10 to 183.5 g/l stepwisely, the fermentation medium was replaced by molasses. The maximum cell mass was 25g/l, and the mean cell mass during the operation was 23.5g/l, which was 3.4 times higher compared with a conventional batch system. The optimum fermentation conditions with feeding molasses of 180g/l were obtained when the fermentation was carried out at 500 rpm and at the dilution rate of 0.037 h^-1. Under these conditions we could safely operate the fermentor for 645 h without any trouble. The maximum alcohol productivity was 4.9g/l·h with an alcohol concentration of 53.9g/l at the dilution rate of 0.091 h^-1.
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Pilot Scale Multi-stage CSTR에서 전분질 원료의 연속 에탄올발효
남기두,이인기,조훈호,최명호,김운식 한국산업미생물학회 1992 한국미생물·생명공학회지 Vol.20 No.3
보다 경제적인 공정개발을 위하여 pilot scale의 다단 연속발효공정(multi-stage CSTR)에서 전분질 원료의 에탄올 발효를 연구하였다. 에탄올 발효를 쌀보리는 30일, cassava는 60일 동안 성공적인 운전을 할 수 있었으며, 원료는 저온 증자를 위하여 20-mesh sieve를 통과한 가루를 사용하였다. 최적화된 운전 조건하에서 cassava의 overall productivity는 에탄올 농도 9.51%(v/v)에서 1.27g/ℓ·h로써 종래 산업적 규모의 회분식 공정에서 얻은 것보다 약 2배 증가되었다.c In order to develop more economic processes, continuous ethanol fermentation from starchy raw materials in a pilot scale multi-stage CSTR was investigated. Ethanol fermentation could be successfully operated for 30 days with naked barley and 60 days with cassava, respectively. Starchy raw materials used for this study were ground and passed through a 20-mesh sieve for low temperature cooking. Under the optimized conditions, the overall productivity of cassava was 1.27g/ℓ·h with an ethanol concentration of 9.51% (v/v), which was higher about 2 times than that obtained from a conventional batch system in industrial scale
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Pilot Scale Multi-stage CSTR에서 전분질 원료를 이용한 알콜 생산
남기두,이인기,조훈호,김운식,서근학,류병호 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.1
쌀보리의 연속 발효에서 효모균체 농도를 증가시켜 발효를 촉진코저 S. cerevisiae IS-019의 단독배양(SCM), IS-019와 응집능력이 있는 S. uvarum IS-026을 혼합배양(MCM) 및 발효 mash 재순환법(MRM)을 검토하였다. 혼합 배양법에서 약 10%의 균체가 대조구보다 증가하였으나 최종 알코올농도는 약간 감소하였다. 단독배양에서 발효조 V_6에서 V_5로 7ℓ/h 유량으로 재순환시켰을 때 균체는 발효조에 따라 140∼170×10exp(6) cells/㎖가 분포되었으며 대조구보다 약 20% 정도 증가하였다. 이 조건하에서 V_5에서 최고 생산성은 알코올농도 7.6%에서 12.16 g/ℓ·h였고, overall 생산성은 알코올농도 8.94%에서 1.19 g/ℓ·h였다. 보다 높은 효모균체 농도유지를 위해서는 Pilot scale multi-stage CSTR에서 mash 재순환과 효모 단독배양이 효과적이였다. In order to induce the rapid alcohol fermentation through the increases of the cell density in a continuous alcohol fermentation of naked barley, the single-cultivation with S. cerevisiae IS-019(SCM, ordinary control), mixed-cultivation with Saccharomyces uvarum IS-026 having a flocculent ability and S. cerevisiae IS-019(MCM), and mash recirculation by single-cultivation of S. cerevisiae IS-019(MRM) modes were investigated. The cell mass in the mixed-cultivation mode was about 10% higher than that of ordinary control but the final alcohol yield was slightly decreased. When recycled the mash with the flow rate of 7 ℓ/h from V_6 to V_5 fermentors under the ordinary control, the cell density was distributed at 140∼170×10exp(6) cells/㎖ depending upon the fermentors-orders, higher about 20% than that of the ordinary control. Under these conditions the alcohol productivity of the maximum and the overall was 12.16 g/ℓ·h with an alcohol of 7.6% at the V_5 fermentor and 1.19 g/ℓ·h with an alcohol of 8.94%, respectively. For higher cell mass it was more effective to apply the mash recirculation mode with the single-cultivation of S. cerevisiae IS-019 in a pilot scale multi-stage CSTR.
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