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생리활성능을 지닌 사람 인터루킨 - 1β - 재조합 단백질의 대장균의 생산
박순희,박한길,나도선,최인성,정태화 ( Soon Hee Park,Han Kil Park,Doe Sun Na,In Seong Choe,Tai Wha Chung ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.3
We produced human recombinant interleukin-1β in E. coli to study the mechanism (s) of its pleiotropic action(s), and to develop it as an anti-cancer or anti-inflammatory drug. We constructed expression vectors using lambda P_L promoter. Expression of hrIL-1β in E. coli was induced and the production of the protein was confirmed by Western blot assay. We also confirmed the presence of biological activity of recombinant IL-1β protein by demonstrating its growth promoting effect on mouse thymocytes.
Anti-IL-1beta 단일클론 항체를 이용해서 발열환자의 뇨중 IL-1beta inhibitor의 확인
남경수(Kyung Soo Nam),배윤수(Yoon Soo Bae),남명수(Myoung Soo Nam),오은숙(Eun Sook Oh),박순희(Soon Hee Park),최인성(In Seong Choe),정태화(Tai Wha Chung) 대한약학회 1993 약학회지 Vol.37 No.4
To effectively purify of IL-1 inhibitor from human febrile urine, we have established monoclonal antibody that reacts with human recombinant interleukin 1beta(IL-1beta). The antibody, designated ON-1, was highly specific to IL-1beta and no cross-reaction with other cytokines(IL-1alpha and IL-4) was observed. As the results of ELISA inhibition assay and Western blotting method, it was further identified that ON-1 had high binding specificity with IL-1beta. IL-1 receptor binding material from febrile patient urine was effectively purified with affinitv column chromatography which conjugated with ON-1. This urinary material inhibited the thymocyte proliferation in a dose-dependent manner. IL-1beta induced thymocyte proliferation activity was inhibited to 67.3% at 6mcg of the purified urinary material. The result may suggest that this urinary material the purified urinary material. The result may suggest that this urinary material will have antagonic effect on IL-1 action mechanism and act IL-1beta inhibitor.