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정시련(See-Ryun Chung),전경희(Kyung-Hee Jeune Chung),김경애(Kyong-Ae Kim) 한국생약학회 1981 생약학회지 Vol.12 No.1
White kidney bean (Phaseolus vulgaris, C, W. K.B.) and Cercis chinensis(C.C) belonging to the Leguminosae contained strong hemagglutinating proteins. The crude lectin from W.K.B. were purified by DEAE Sephadex A-50 column chromatography. The 0.1M peak from this procedure demonstrated acceptable criteria, e.g., yield, optical density and hemagglutinating activity and three bands were observed in polyacrylamide disc gel electrophoresis. Further purification was achieved by hydroxyapatite column chromatography and strong hemagglutinating activity were recovered in 0.1 M peak. I was electrophoresed and found to migrate as a single band. These suggests that the condition of purification for W.K.B. lectin was encourageable but these methods did not brought agreeable satisfaction to the Cercis chinensis lectin.
해양 천연물로부터 면역기능 조정제 렉틴 개발: MLA-I, MLA-II, MLA-III의 특성
정시련(See Ryun ChUng),김장환(Jang Hwan Kim),전경희(Kyung Hee Jeune) 대한약학회 1995 약학회지 Vol.39 No.3
Three new lectins, MLA-I, MLA-II and MLA-III, have been isolated and purified from the hemolymph of Meretrix lusoria and reported previously. Biophysicochemical characteristics were investigated with these three MLA lectins. The MLA lectins agglutinated human erythrocytes nonspecifically and proved as D-galactose group carbohydrate specific. Molecular weight of MLA-I, II and III were estimated to be 330, 500 and 31OKD, respectively, by gel filtration on Sepharose CL-6B column. On SDS-polyacrylamide gel electrophoresis, MLA I was dissociated into a single subunit of 42KD, MLA-II was into the twelve subunits of 46, 32, 30, 28, 25, 23, 22, 20, 19, 16, 15, and 14KD, and MLA-III was into the two subunits of 72 and 44KD. The pI of MLA-I, II, III were 4.0, 4.9 and 5.0. Amino acid analysis revealed a high contents of acidic and hydroxy amino acids, and a paucity of sulfur containing amino acids. Proline was not contained in MLA-II.
정시련,손경숙,소명숙,전경희,Chung, See-Ryun,Son, Kyeun-Suk,So, Myung-Suk,Jeune, Kyung-Hee 생화학분자생물학회 1987 한국생화학회지 Vol.20 No.3
밤고둥의 생리식염수용액 추출물은 사랑 및 각종 동물 적혈구를 비특이적으로 응집시켰으며 림프구도 응집시켰다. 밤고둥의 새로운 렉틴 성분을 생리식염수용액추출, 황화암모늄 침전, 이온교환 크로마토그래피 등의 방법으로 분리 정제하였다. 이는 전기영동상에서 하나의 주된 띠와 다른 몇개의 띠를 나타내었으며 주된 띠에서만 렉틴활성을 나타냈다. 부분 정제된 밤고둥 렉틴은 pH변화에는 비교적 안정하였으나 열에는 불안정하였다. 적혈구 응집은 lactose와 D-galactose의 각각 10mM과 100mM농도에서 저해되었다. 또한 밤고둥 렉틴은 마우스의 림프구에 대한 분열자극효과를 나타냈다. The whole body extracts of a top shell, Chlorostoma argyrostoma lischkei, agglutinated nonspecifically human and other animal erythrocytes and also agglutinated rat and murine splenic lymphocytes. New lectin was purified by the following procedures: 0.15M NaCl extraction, salt fractionation and ion exchange column chromatographies. The purified lectin showed one major and a few minor bands in polyacrylamide gel electrophoresis, but in only one major band had lectin activity. The partially purified lectin was relatively stable at various pH, but heat labile. Among the tested sugars, only lactose and D-galactose inhibited lectin activity at a concentration of 10mM and 100mM, respectively. The lectin was a mitogenic toward murine splenic lymphocytes
해양패류렉틴 ( Ⅶ ) 밤고둥 ( Chlorostoma argyrostoma lischkei ) 렉틴의 부분 정제 및 특징
정시련,손경숙,소명숙,전경희 ( See Ryun Chung,Kyeung Suk Son,Myung Suk So,Kyung Hee Jeune - Chung ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.3
The whole body extracts of a top shell, Chlorostoma argyrostoma lischkei, agglutinated nonspecifically human and other animal erythrocytes and also agglutinated rat and murine splenic lymphocytes. New lectin was purified by the following procedures: 0.15M NaCl extraction, salt fractionation and ion exchange column chromatographies. The purified lectin showed one major and a few minor bands in polyacrylamide gel electrophoresis, but in only one major band had lectin activity. The partially purified lectin was relatively stable at various pH, but heat labile. Among the tested sugars, only lactose and D-galactose inhibited lectin activity at a concentration of 10mM and 100mM, respectively. The lectin was a mitogenic toward murine splenic lymphocytes.
해양패류로부터 렉틴성분 개발연구 조각매물고둥 렉틴의 분리 , 정제 및 특성
정시련,김장환,전경희 ( See Ryun Chung,Jang Hwan Kim,Kyung Hee Jeune - Chung ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.4
A New lectin from shellfish, Neptunea intersculpta, was purified by the following steps; 0.15 M NaCl extraction, (NH₄)₂SO₄ precipitation, DEAE-cellulose, hydroxyapatite and gel filteration on Sephadex G-100 column chromatography. The NIA (Neptunea intersculpta)lectin was shown one major band and two faint minor bands on polyacrylamide gel electrophoresis. Agglutinating activity was specifically inhibited by lectose and no carbohydrate was found by anthrone test and thin layer chromatography. Immunochemical techniques were employed to examine structural similarities between NIA lectin and other crude lectin. The result of immunodiffusion showed that NIA lectin was partialy identified with white kidney bean crude lectin, but was not identified with Agaricus bisporus crude lectin. Further characterization of the biological and chemical properities of this lectin are still in progress.
한국산 식물 자원으로 부터 새로운 렉틴 성분의 분리 정제 ( 1 ) 콩과 식물의 렉틴
정시련,전경희 ( See Ryun Chung,Kyung Hee Jeune Chung ) 생화학분자생물학회 1981 BMB Reports Vol.14 No.3
In order to find new lectins from Korean natural products, 55 kinds of plants belonging to 31 families were screened by using several different type of blood cells. Phaseolus vulgaris C(W, K. B.) and Cercis chinenesis of Leguminosae contained strong hemagglutinating proteins. The crude lectins from W. K. B. were purified by DAEA Sephadex A-50 column chromatography. The 0.1 M peak from this procedure demonstrated acceptable criteria, e. g. yield, optical density and hemagglutinating activity and three bands were observed in polyacrylamide disc gel electrophoresis. A further purification was achieved by hydroxyapatite column chromatography and strong hemagglutinating activity was recovered in 0. 1 M peak. It was elecrophoresed and found to migrate as a single bans. The condition of purification for W. K. B. lectin was encourageabie but did not brought agreeable satisfaction to the Cercis chinensis lectin.
담배 성분에 관한 연구 - 니코틴 함량 변화에 미치는 K , Ca 및 Mg의 조성 비율
정시련(See Ryun CHUNG),전경희(Kyung Hee JEUNE) 한국생약학회 1973 생약학회지 Vol.4 No.4
Tobacco, which has nicotine for its main component, has been in medical use for a long time and in great demand for smoking throughout the world. The purpose of this study is to control nicotine amount and to increase yield more efficiently by the method of systematic variations. Nutrient solutions for tobacco culture were designed and prepared in 10 kinds with mineral ions: NO<sub>3</sub><sup>-</sup>, SO<sub>4</sub><sup>--</sup>, PO<sub>4</sub><sup>-3</sup>, K<sup>+</sup>, Ca,<sup>++</sup> Mg,<sup>++</sup> Mn, B, Cu, Zn, Mo, and Fe. Nicotiana tabacum L. Yellow Special A, grown for 50 days from sowing, was replanted in plastic pot and cultured for 65 days supplying with prepared nutrient solutions. After harvest, their nicotine amount was determined by means of acid-base titrimetry and gas chromatography. The tobacco plants in KCa 4 and KMg 9 groups demonstrated the highest yield in total leaves weight and KMg 7 group in average nicotine amount. They have shown the increase of nicotine amount from lower leaf to 16th leaf, and thereafter decreased gradually. The author also pursued the optimum ionic proportions for the absolute nicotine amount in tobacco by means of systematic variations method.
정시련(See Ryun Chung),정수민(Su Min Chung),이승호(Seung Ho Lee),전경희(Kyung Hee Jeune) 대한약학회 1996 약학회지 Vol.40 No.4
The erythrocytes agglutination test was applied to the common korean plants for lectin activity screening by using human blood. During the four years, 108 species from 46 families of floras were collected, identified and subjected to the test after being divided into several different parts. Only 13 species demonstrated strong lectin activities. Meanwhile 66 species did not shown any agglutination. All others were observed as having low activity or as having hemolytic constituents.