이질균의 약제 내성과 R-plaxmid

정규선(Kyu Sun Chung),변혜경(Hye Kyung Byun) 대한약학회 1990 약학회지 Vol.34 No.1

These studies were made to assess the present stage of resistance of Shigella species to antibiotics and to find characteristics of R-plasmid of these bacteria. From 1986 to 1988, 125 strains of Shigella species were isolated from patients specimens collected in Chung Cheong-do Hospital, Kyunghee Medical Center, city an provincial health and environmental institutes. These specimens were tested for resistance to 12 kinds of antimicrobial agents by agar dilution method. Using Muller-Hinton agar for the assay of drug resistance and Trypticane Soy Broth as propagating medium for conjugation. All the strains(100%) were resistant to one or more antibiotics. Drug resistance patterns of isolated strains were found as the highest resistance to ampicillin(98%) in 1986, to tetracycline(98%) in 1987, to tetracycline(100%) in 1988, all strains were sensitive to gentamicin, amikacin, tobramycin. Chronologically, resistance decreased gradually as it was shown in relation to kanamycin, rifampicin in 1986, 1987 and 1988, (4%, 2%) (4%, 2%) (O%, 0%) respectively. But, resistance was increased year by year as it was shown in relation to tetracycline, nalidixic acid, streptomycin in 1986, 1987, 1988 (89%, 19%, 45%) (98%, 46%, 71%) and (100%, 58%, 88%). The resistance in correlation to more than 5 drugs, which was 13 strains among 47 strains in 1986, 38 strains among 87 strains in 1987, 23 strains among 26 strains in 1988, was increased gradually. In the transfer test of drug resistance by conjugation methods, the rate which was 3 strains(50%) in 1986, 8 strains (62%) in 1987, 3 strains (100%) in 1988, was increased gradually. When the donor strains were conjugated with the recipient strains, the conjugation rate was high in the multiple resistant strains. The relationships of transferring patterns of drug resistance and molecular weight of R-plasmid were variable. However, only a plasmid which has more than 35 Mgd was transferred.

세균의 약제 감수성에 관한 연구 II

정규선(Kyu Sun Chung) 대한약학회 1986 약학회지 Vol.30 No.2

A total of 130 of Staphylococcus strains isolated from various clinical specimens of admitted patients of a university hospital with systemic or severe cases of infection. All of these were tested for the antimicrobial susceptibility to 11 drugs of common use. The hospital strains isolated showed higher frequency of resistance against four drugs including gentamicin, penicillin, erythromycin, kanamycin but amikacin, cephalothin, streptomycin were effective. And also 47.7% of methicillin-resistant Staphylococcus were isolated from the clinical patients. However, isolated Escherichia coli strains showed higher frequency of resistance, but two drugs, tobramycin and gentamicin were effective to them.

고려홍삼 추출성분이 Leucocytes 유주능에 미치는 영향

정규선(Kyu-Sun Chung) 大韓微生物學會 1997 大韓微生物學會誌 Vol.32 No.1

가축 및 하천에서 분리한 대장균의 항균제내성

정규선(Kyu Sun Chung),이연태(Yeon Tae Lee),표석연(Seog Youn Pio) 대한약학회 1989 약학회지 Vol.33 No.2

One hundred and sixteen strains of E. coli isolated from drainage in Seoul city in 1987 and 104 strains of E. coli isolated from stools of domestic animals in suburb of Seoul in 1987 were examined for susceptibilities to 10 antimicrobial agents by the agar dilution method. The susceptibilities of the two groups to each antimicrobial agent were compared and correlations in the antimicrobial susceptibility of the 220 strains of E. coli among the 10 antimicrobial agents were also analyzed. The frequency of resistance strains was highest to tetracycline with 77%, and followed by chloramphenicol, streptomycin, ampicillin, kanamycin and cephalosporin in the decreasing order, changing from 62% to 10%. Strains resistant to tobramycin, nalidixic acid, gentamicin and amikacin occupied 3 strains, 2 strains, 2 strains and 1 strain respectively.

한국산 고등 균류의 성분에 관한 연구

정규선(Kyu Sun Chung) 대한약학회 1979 약학회지 Vol.23 No.3,4

Free amino acids in extracts and total amino acids in hydrolysates of eight species of higher fungi were analyzed by and amino acid autoanalyzer. Twenty amino acids were analyzed in eight species of higher fungi. 1) of all free amino acids contained in higher fungi, alanine is the richest, and then comes glutamic acid, serine, arginine, proline and histidine in that order. Of all total amino acids contained in higher fungi, glutamic acid is the richest, and then comes proline, valine, aspartic acid, alanine, leucine in that order. Especially Russula fragilis and Lepiotaprocera contain large quantity of glutamic acid. 2) Gross contents of free amino acids in the extracts is high in order of Lepiota procera, Phylloporus rhodoxanthus, Russula fragilis, Tylopilus felleus and total amino acids in hydrolysates is high in Phylloporus rhodoxanthus, Lepiota procera, Russula fragilis, Lentinus lepideus. 3) Total amount of essential amino acids in the extracts was high in Lepiota procera, Phylloporus rhodoxanthus, Tylopilus felleus, Xylaria hypoxylon, Lentinus lepideus, Russula fragilis, Lactarius piperatus and Calocybe gambosa and in the hydrolysates of Phylloparus rhodoxanthus, Russula fragilis, Lepiota procera, Tylopilus felleus, Lentinus lepideus, Lactarius piperatus, Calocybe gambosa and Xylaria hypoxylon in that order, respectively.

잣버섯 균사체로부터 분리한 수용성 단백다당체 Lepidan의 면역증가작용

진미림(Mi Rim Jin),정규선(Kyu Sun Chung) 대한약학회 1999 약학회지 Vol.43 No.5

In this study, we investigated the immunopotent activities of lepidan, a water soluble proteolycan from Lentinus lepideus. To examine whether lepidan may affect nonspecific immune responses, we determined the effect on the production of nitric oxide (NO). Lepidan effectively increased the No production in IFN-gamma and LPS triggered RAW 264.7 cells. To further demonstrate the evidence that lepidan activates various immune cells, we determined the alkaline phosphatase activity, plaque-forming cell nember and secretion of interleukine-4 (IL-4) and granulocyte/macrophage-colony stimulating factor (GM-CSF) after lepidan treatment in murine splenocytes. The results showed that lepidan increased alkaline phosphatase activity and the number of plaque-forming cells, which indicates that lepidan can lead B lymphocytes to late stage of differentiation. Also, when the splenocytes were cultured with lepidan for 48hr, the level of IL-4 and GM-CSF in the supernatant dramatically increased. Taken together, these data suggest that lepidan is a biological response modulator that is able to activate immune responses.

환자, 가축 및 하천에서 분리한 대장균의 약제내성과 전달성 R-plasmid

김현주(Hyun Joo Kim),정규선(Kyu Sun Chung) 대한약학회 1991 약학회지 Vol.35 No.2

These studies were made to assess the present stage of resistance to antibiotics, incidence and transferability of R-factors against E. coli. From March to July 1987, 59 strains of E. coli were isolated from specimens, of patients collected at university hospitals in Seoul, 64 strains from stools of domestic animals and 66 strains from drainages in Seoul. These specimens were tested for resistance to 12 kinds of antimicrobial agents by means of agar dilution method. Using Muller-Hinton agar for the assay of drug resistance and tryptic soy broth as propagating medium for conjugation. The strains of E. coli were found to be resistant to one or more antibiotics and were considered to be potential donors of R-plasmid. The resistant strains of E. coli isolated from patients, domestic animals and drainages were found to be 55(93%), 33(52%) and 31(47%), respectively. Resistance to Tc, Ap and Cb was the highest in those isolated from patients and drainages, and resistance to Tc, Cm and Sm was the highest in those isolated from domestic animals. In the transfer test of drug resistance by conjugation method, 17 strains (47%) isolated from patients, 15(54%) isolated from domestic animals and 15(56%) isolated from drainages showed positive results, transperable resistant plasmid molecules with variable range in each strain.

헬리콘 플라즈마 원을 이용한 우주 추진체의 특성

최근식(Geun-Sig Choi),우현종(Hyun-Jong Woo),정규선(Kyu-Sun Chung),이명재(Myoung-Jae Lee),노태협(Taihyeop Lho),정용호(Yong Ho Jung) 대한전기학회 2005 대한전기학회 학술대회 논문집 Vol.2005 No.11

韓國 一部地域에서 分離된 Slmonella 菌群의 藥劑感受性에 關한 調査

鄭奎善 최신의학사 1976 最新醫學 Vol.19 No.3

The followings are the results of the antibiogram of Salmonella organisms isolated from the Salmonella patients in an outbreak and collected from municipal and provincial hospitals. 1. Among the 103 strains identified as salmonella organisms out of the 266 samples collected, 2 of Salmonella paratyphi A. 89 of Salmonella paratyphi B and Salmonella typhi, 3 of Salmonella typhimurium, and 7 of Salmonella enteritidis were isolated. 2. Salmonella typhi was less sensitive of chloramphenicol terramycin ampicillin and kanamycin. 3. 22 strains strains of the salmonella organisms appeared to be highly tolerant to high concentration of streptomycin.

感染動物의 臟器中의 RNA 및 DNA 含量變化에 對한 硏究

鄭奎善,盧台宣 최신의학사 1970 最新醫學 Vol.13 No.2