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최기준(Gi Jun Choi),임영철(Young Chul Lim),김기용(Ki-Yong Kim),성병렬(Byung Ryul Seong),김맹중(Meing Jooung Kim),김원호(Won Ho Kim),지희정(Hee Chung Ji),이종경(Joung Kyong Lee),전병수(Byoung Soo Jeon),정민웅(Min Woong Jung),이상훈(Sa 한국초지조사료학회 2008 한국초지조사료학회지 Vol.28 No.3
본 시험은 사료용 옥수수 검은줄오갈병 피해 다발지역의 발병실태를 조사하여 종합적인 방제기술 개발에 필요한 기초 자료를 확보하고자, 2006년부터 2007년까지 제주도를 제외한 전국을 대상으로 수행하였다. 검은줄오갈병의 발생은 지역에 따라 차이가 많았으며, 발병이 심한 지역은 경기(이천), 전북(고창, 김제), 전남(영광), 경북(김천, 영주), 충남(천안, 보령)이었다. 옥수수 검은줄오갈병 다발지역에서 파종시기가 4월 26일~5월 15일까지 파종이 발병이 적었으며, 파종기가 4월 10일~20일 또는 5월 31일 이후에서 발병이 많았다. 검은줄오갈병의 발생은 옥수수 품종간의 차이는 다소 있으나 저항성을 나타내는 품종은 없었다. 옥수수 검은줄오갈병 감염에 따른 건물수량은 발병률이 높을수록 유의적으로 감소하였으며, 발병률과 옥수수 건물수량 간에는 고도의 부의상관 (r = -0.86**)이 인정되었다. 검은줄오갈병에 감염된 옥수수는 건전한 옥수수보다 건물소화률은 3.2%, 가소화양분총량은 3.6%, RFV는 12% 낮았으며, NDF와 ADF 함량은 각각 3.7, 4.5% 높아져 전체적으로 사료가치가 낮아지는 것으로 나타났다. This experiment was carried out to investigate the actual outbreak status of rice black-streaked dwarf virus (RBSDV) disease in forage corn for securing the basic data to control of the disease all over the country from 2006 to 2007. Outbreak of the disease showed a wide difference according to regions and was severe in Icheon, Gochang, Kimje, Youngkwang, Kimchun, Youngju, Cheonan, and Boroung provinces in Korea. Outbreak rate of the disease was effected by planting time of forage corn. Disease rate was lower in corns planted from Apr. 26 to May 15 than in those of planted from April 10 to 25 or after May 31. The corn fields planted from April 26 to May 15 showed lower disease rate than that of planted from April 10 to 25 or after May 31. Also the outbreak rate of the disease was some difference according to corn varieties but was not genuinely resistant varieties to RBSDV. Dry matter yield of forage corn was significantly decreased depending on disease rate, and was highly negative correlation to disease rate. The forage corn infected with RBSDV was lower 3.2%, 3.6% and 12% in IVDMD, TDN, and RFV, and higher 3.7% and 4.5% in NDF and ADF than those of the healthy plant, respectively.
초임계 이산화탄소 추출법을 이용한 오징어 가공 부산물로부터 고도불포화 지방산 회수
강성실(Seong Sil Kang),김병준(Byung Jun Kim),전병수(Byung Soo Chun) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.2
A squid viscera oil contains a high content of EPA, DHA, and other valuable polyunsaturated fatty acids. The extractions of squid viscera oil by supercritical carbon dioxide both with/without 3% (v/v) ethanol were performed in a semicontinuous flow extractor at 8.3 to 13.8㎫ and 25 to 50℃. When ethanol was added to SC-CO₂, the extraction ratio of lipid increased. The extracts contained high content of unsaturated oils like DHA and EPA. The highest extraction yield of lipid from squid viscera oil extracted by supercritical carbon dioxide was obtained at 12.4 ㎫ and 40℃ with/without entrainer. The main fatty acids of squid viscera oil extracted by supercritical carbon dioxide were myristric acid (14:0), palmitic acid (16:0), palmitoleic acid (16:1), oleic aad (18:1), arachidic acid (20:0), eicosapentaenoic acid (20:5), and docosahaxaenoic acid (22:6).
초임계 이산화탄소를 이용하여 멸치(Engraulis japonicus)로부터 회수된 고농축 단백질의 특성
이승미 ( Seung Mi Lee ),윤준호 ( Jun Ho Yun ),이주희 ( Joo Hee Lee ),전병수 ( Byung Soo Chun ) 한국수산과학회 2012 한국수산과학회지 Vol.45 No.3
Supercritical carbon dioxide (SCO2) extraction was used to recover concentrated proteins and to remove lipids and odor causing compounds from anchovy. Engraulis japonicus SCO2 was used as the solvent for extraction, which was performed in a semi-batch flow reactor. The experimental conditions used were pressure, 15-25MPa; temperature, 40-60℃ and sample size, 500um. The proteins obtained under these conditions performed well in a sensory evaluation; moreover, effective lipids and odor removal was achieved. The stability and characteristics of the proteins recovered with different solvents were also evaluated. The samples were sterilized by processing with SCO2, Escherichia coli was not detected after storage for several days. The sensory characteristics were found to be superior to those of a sample produced by hexane extraction. Thus, the protein concentrate was obtained at 60℃ and 25MPa was deemed valuable as a foodstuff.
초임계 이산화탄소를 이용하여 추출된 멸치 오일의 지방산 조성 및 산화 특성
이승미(Seung-Mi Lee),윤준호(Jun-Ho Yun),전병수(Byung-Soo Chun) 한국청정기술학회 2011 청정기술 Vol.17 No.3
초임계 이산화탄소 및 유기용매를 이용하여 동결 건조된 멸치 시료로부터 오일을 추출하였으며, 시료는 700 μm로 균질화시켜 사용하였다. 다양한 압력 (15~25 MPa) 및 온도 (40~60 ℃) 조건에서 실험을 수행하였으며, 22 g/min의 일정한 유량을 추출기로 유입시켰다. 추출된 오일의 지방산 조성을 확인하기 위하여 가스 크로마토그래피를 이용하여 분석한 결과 추출된 멸치 오일에는 myristic acid, palmitic acid, stearic acid, palmitoleic acid, eicosapentaenoic acid (EPA), 그리고 docosahexaenoic acid (DHA)가 주요 지방산으로 확인되었다. 추출된 오일 내 EPA 및 DHA와 같은 고도불포화지방산(PUFAs)함량을 비교하였을 때, 유기용매 추출 오일보다 초임계 이산화탄소를 이용하여 추출한 오일의 고도 불포화 지방산 함량이 더 높았다. 산화도 비교 실험인 산가 및 과산화물가 값을 측정한 결과, 유기용매 추출 오일보다 초임계 이산화탄소를 이용한 추출 오일의 산화도가 유의적으로 낮았으며, 산화되는 속도 또한 느리게 진행되는 것을 확인할 수 있었다. Anchovy oil was extracted using supercritical carbon dioxide (SCO₂) and organic solvents. Extraction was carried out at temperature range from 40 to 60 ℃, and pressure range from 15 to 25 MPa. The flow rate of CO₂ (22 gmin<SUP>?1</SUP>) was constant entire the extraction period of 1.5 h. The fatty acid composition of anchovy oil was analyzed by gas chromatography (GC). The main fatty acids of anchovy oil were myristic acid, palmitic acid, stearic acid, palmitoleic acid, EPA (eicosapentaenoic acid), and DHA (docosahexaenoic acid). In addition, the oil obtained by SCO₂ extraction contained a higher percentage of polyunsaturated fatty acids especially EPA and DHA comparing to the organic solvent extracted oil. The oxidative stability of oils extracted from Anchovy by SCO₂ extraction was compared to those extracted by organic solvents. Results showed that the storage periods of oils obtained by SCO₂ extraction were longer than those of organic solvents extraction.