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이유선 ( Yu Seon Lee ),박인주 ( Inju Park ),신서영 ( Seoyoung Shin ),최근화 ( Keunhwa Choi ),유지현 ( Ji Hyun Yoo ),이정미 ( Jung Mi Lee ),장귀현 ( Gui-hyun Jang ),문귀임 ( Gui Im Moon ),허수정 ( Soojung Hu ),전대훈 ( Daehoon Jeon 한국환경농학회 2022 한국환경농학회 학술대회집 Vol.2022 No.-
As a herbicide that controls cell division by interfering with the synthesis step of very long-chain fatty acids constituting the cell wall, Pethoxamid is used before or just after germination of crops such as rice and corn. It has a short half-life and low resistance, so it is very effective in removing a wide range of weeds. In this study, an analytical method for the determination of Pethoxamid in foods and agricultural products was developed as Ministry of Food and Drug Safety(MFDS) method. We used five agricultural products(mandarine, potato, soybean, hulled rice, and chilli pepper) as group representative to verify for this study. The sample preparation process was conducted in two steps with QuEChERs(quick, easy, cheap, effective, rugged, and safe) method for making the extraction and purification process efficient. First, in order to extract the samples, 0.1% formic acid in acetonitrile was added and shaken for 10 minutes. Next, magnesium sulfate anhydrous and sodium acetate were added, followed by centrifugation(4,700 G, 10 minutes). Thereafter, to remove the interferences in samples, dispersive solid-phase extraction(d-SPE) using C18, magnesium sulfate anhydrous, PSA and GCB was performed for 10 minutes before LC-MS/MS(Liquid Chromatography-Tandem Mass Spectrometry) analysis with C18 column. The linear standard calibration curves were confirmed showing r-square values higher than 0.99(coefficient of determination with calibration range). Mean average accuracies of Pethoxamid were shown 78.8∼95.2%. The precision were also shown less than 7.5% for all five samples. These results are suitable for CODEX guideline for residue analysis(CAC/GL 40). In summary, the proposed method for determination of Pethoxamid residue in foods could be included in the Korean Food Code for enabling the safety management of pesticides.
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