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- 저자 : 이효종(H. Lee) (검색결과 35 건)
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산양의 수정란 이식 및 조작기법 개발에 관한 연구 5 . 생쥐 및 산양 분할 수정란의 생존성 및 이식 후 수태율의 향상
박충생(C . S . Park),최상용(S . Y . Choe),이효종(H . J . Lee),이지삼(J . S . Lee),박희성(H . S . Park) 한국축산학회 1991 한국축산학회지 Vol.33 No.5
This experiment was conducted to compare different procedures for improvement of viability of embryos after bisection and to investigate some factors affecting development of the bisected embryos. Demi-embryos were successfully produced by micromanipulation of mouse and goat embryos at stages from morula to blastocyst. In mouse, a superior viability(90%) of demi-embryos was achieved by culturing expanded blastocysts after bisection. The highest pregnancy rate(57.1%) and implantation rate(28.2%), which were almost same as the rates of intact embryos. were also achieved by transferring the demi-embryos at this stage after culture for 24 h in vitro. Twenty -eight 3%) of 30 embryos collected from superovulated Korean native goats were successfully bisected, of them 23 demi-pairs were transferred to recipients. Six and 3 does of them were diagnosed pregnant on 21 and 60 days of gestation, respectively. The present result indicates that in goat, hatched blastocysts might be more suitable for successful bisection to produce monozygotic twins than morulae or earlier blastocysts.
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노면굴곡형상 측정용 트레일러설계를 위한 조향특성에 관한 연구
김형윤(H.Y.Kim),이효종(H.J.Lee) 한국자동차공학회 1993 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-
The special trailor for measuring the paved and unpaved road profiles is designed to instaneously obtain the power spectral and coherecy of the<br/> right and left vehicle tracks. In this study, we find and determine the design prameters of the steering radius and dynamics of the trailor towed<br/> by a vehicle. The equations of the trailor steering dynamics where we use the exponential function in relation of tire slip angle and conering force is calculated numerically, and the vertical load of wheels and<br/> drawing force is reviewed.<br/>
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소의 Immunoglobulin G1 에 대한 단 Clone 성 항체의 생산에 관한 연구 2 . 소의 Immunoglobulin G1 에 대한 단 clone 성 항체의 생산
김정우,여상건,이효종 ( J . W . Kim,S . G . Yeo,H . J . Lee ) 한국축산학회 1986 한국축산학회지 Vol.28 No.12
Spleen cells from Balb/c mice immunized with bovine IgG1 heavy chain were successfully fused with SP2/0 mouse myeloma cells and culture fluid of the hybrid cells were tested for specificity by enzyme-linkers immunosorbent assay. Of 48 initial hybrid clones, 21 produced antibodies to bovine IgG1. After cloning and rectoning, 2 (G1D2, G1H12) of these hybrid clones retained specificity and were grown in mass culture. The monoclonal (anti-bovine IgG1) antibody G1D2 and G1H12 were reacted to bovine IgG1 specifically in their dilution of more than 100- and 10-folds, respectively.
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생쥐 8- 세포기 수정란의 핵이식에 의한 복제산자의 생산
최상용(S . Y . Choe),박희성(H . S . Park),이효종(H . J . Lee),박충생(C . S . Park) 한국축산학회 1992 한국축산학회지 Vol.34 No.2
The present study was carried out to develop a technology of cloning mammalian embryos using mouse embryos as a model and to produce cloned offsprings by transfer of reconstituted embryos to recipient females. Single nucleus from eight-cell embryos was transplanted into the enucleated two-cell embryos by micromanipulation and Sendai virus-mediated fusion. The fusion of nuclei with recipient cytoplasm and the development of reconsitituted embryos in vitro as well as in vivo were examined. The results obtained were summarized as follows: A total of 95(59.3%) nuclei from 20 donor embryos of 8-cell stage was injected successfully into the enucleated 2-cell recipient embryos, of which octa-. hepta-, hexa-, penta- and quadruplets were 0. 2, 3, 3 and 12 sets, respectively. The success in nuclei injection was lower(P$lt;0.05) from 8-cell donor embryos than 4-cell or 2-cell donor embryos. Among the embryos injected with nuclei a total of 81(85.2%) embryos were fused successfully with donor embryos of 8-cell stage. From donor embryos of 8-cell stage, 2 hexa-, 2 penta-, 11 quadru- and 5 triplets were fused successfully. A total of 46(39.l%) reconstituted embryos from 8-cell donor nuclei were developed in vitro to blastocysts, of which penta-, quadru- and triplets. twins and singles were 1, 2, 6, 6 and 3 sets, respectively. The development in vitro to blastocysts of the reconstituted embryos was lower (P$lt;0.05) from 8-cell donor embryos than 4- or 2-cell donor embryos. A total of 13(27.l%) cloned live youngs from transfer of cloned embryos using 8-cell donor embryos were produced, of which triplets, twins and singles were 2, 3 and 1 sets, respectively. The percentage of cloned youngs produced from transfer of cloned embryos using 8-cell donor embryos was lower(P$lt;0.05) than 4- or 2-cell donor embryos. The mean number of cloned live youngs produced per 8-cell donor embryo used for nuclei transplantation was 0.81.
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산양의 수정란 이식 및 조작기법 개발에 관한 연구 2 . 산양의 분만 및 유산 후의 단발정주기
박충생(C . S . Park),최상용(S . Y . Choe),이효종(H . J . Lee),이지삼(J . S . Lee) 한국축산학회 1991 한국축산학회지 Vol.33 No.4
This experiment was conducted to investigate the incidence of short estrous cycle and control of short cycle in Korean native goats. Abortion was induced by a single i.m. injection of 3 ㎎ PGF₂α at late stage of gestation. Does after parturition or abortion were teased twice daily with vasectornized bucks and behavioral estrus was recorded. Serum progesterone concentration was analysed by RIA. Mean post-partum or post-abortion interval to first estrus was shortest as 11.9 days in PGF₂α-aborted does compared to 22.1 days in does isolated from their kids within 7 days after parturition and 26.8 days in suckling does. Duration of this first standing estrus was not significantly different among groups. Four limes daily injections of 25 ㎍ Gn RH to does for Day 3 to 5 of the first estrous cycle following parturition or abortion ruduced significantly(p$lt;0.0l) the incidence of short estrous cycle to 38.5% compared to 80.0% in control group of does. Mean concentration of serum progesterone in does showing short estrous cycle was found to be basal level at estrual phase but considerably high level up to 1.06 ㎎/㎖ on Day 3 to 4 of the cycle. These results indicate that the short estrous. cycle may he resulted from the premature luteal regression and GnRH treatment during early luteal phase of the estrous cycle can partly prolong the short lifespan of corpora lutea.
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산양의 수정란 이식 및 조작기법 개발에 관한 연구 4 . 생쥐 및 산양 수정란의 분할조작에 의한 일란성 쌍태유기
박충생(C . S . Park),최상용(S . Y . Choe),이효종(H . J . Lee),이지삼(J . S . Lee),박희성(H . S . Park) 한국축산학회 1991 한국축산학회지 Vol.33 No.4
This experiment was conducted to establish a simple technique for bisecting mouse and goat embryos of morula to blastocyst stage and to develop a better culture and transfer system of demi-embryos for the production of identical twins. Mouse morulae or blastocysts and goat blastocysts were easily bisected without pronase treatment, using a microblade fixed to a micromanipulator. Bisected mouse embryos developing normally during culture were 71.4% of a total of 319 morulae and blastocysts. Survival rate of demi-embryos(29.0%) after culture and surgical transfer to recipient mice was comparable to result of intact embryos(26.0%). More transferable embryos (3.5±0.8/donor) were recovered in 7 to 8 days after mating when the does were induced to superovulate by injection of FSH-p in decreasing doses and progesterone priming for 10 days. and this superovulation regimen resulted in very low incidence of short estrous cycle(6.7%). Three kids were produced from 2 of 6 does received intact embryos. Fourteen pairs and one-half of demi-embryos were transferred to 15 recipients. of which one recipient delivered a single female kid.
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