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3차원 세포 장벽의 동적의 미세환경을 구축하기 위한 다층구조 플랫폼 개발
임정은(Jungeun Lim),이형록(Stephen Rhee),전누리(Noo Li Jeon) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.11
Cellular barrier layers play an important role in the body in regulating the diffusion between two microenvironments. Biological barriers such as the blood-brain barrier, blood-cerebrospinal fluid barrier, and the small intestine villi all utilize these epithelial barriers to control the diffusion of solutes into their system. This research describes a method to analyze the effects of microfluidic flow on cellular barriers through the use of a pumpless rocker. A barrier layer was recapitulated using brain epithelial cells cultured on a 3D-printed mesh platform, and a rocker system was used to induce microfluidic flow within the system. This study can provide insight into the mechanisms and effects of microfluidic flow on epithelial barriers for future studies.