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배양기내 GAS 분압의 조성이 소 체외수정란의 체외발육에 미치는 영향에 대한 연구
이원유,신태영,이병천,황우석 韓國受精卵移植學會 1995 한국동물생명공학회지 Vol.10 No.2
To examine the critical effect of oxygen concentration on embryonic development, in vitro fertilized embryos were cultured in media(TCM199 vs. SOF) supplemented sera(1O% FCS vs. 10% HS) with and without bovine oviduct epithelial cells under two gas atmosphere (5% in air vs. 5% , 5% , 90% ). Oocytes, obtained from abattoir ovaries, were matured in EGF containing TCM199 medium co-cultured with BOEC for 24 hours, followed by exposure to frozen-thawed, heparin4reated spermatozoa in TALP for 30 hours. And then early embryos(1~2 cell) were cultured in both TCM199 and SOF supplemented with 10% FCS or 10% RS under 5% in air or 5% COi, 5% , 90% . Development to morulae and blastocysts was recorded on days 7, after the start of in vitro fertilization. The developmental rates of in vitro fertilized embryos to morulae and blastocysts cultured in SOF with BOEC under 5% , 5% , 90% (24.4%) were significantly(p<0.05) higher than cultured in SOF with BOEC under 5% in air(14.1%) at seven days after in vitro fertilization. When early bovine embryos were cultured in TCM 199 and SOF under two different gas atmosphere, there were no significant differences in the developmental rates to morulae and blastocysts between supplements of 10% FCS and 10% HS. The rates of development to morulae and blastocysts were significantly(p<0.01) higher in TCM 199 with BOEC(24.7%) than TCM199 without BOEC(10.9%) under 5% in air, otherwise SOF without BOEC(36.4%) were significantly (p<0.05) higher than in SOF with BOEC (24.4%) under 5% , 5% , 90% . In summary, these experiments have proved that the culture system in SOF supplemented 10% ES is effective on in vitro development of early bovine embryos under 5% , 5% , 90% . In addition, it is effective to development of bovine embryos that TCM 199 should be co-cultured with BOEC and SOF should be cultured without somatic cells under two different gas atmosphere.
백서의 구치 이동 초기에 Prostaglandin E<sub>2</sub>와 Evening Primrose Oil의 영향에 관한 형태학적 연구
이원유,Lee, Won-Yu 대한치과교정학회 1990 대한치과교정학회지 Vol.20 No.1
To study the effect of prostglandin $E_2$ and evening primrose oil on orthodontic tooth movement in rats, one hundred and sixty rats were divided into four groups of 40 rats each. One group, injected with saline on the palate subperiosteally, served as a control group. A second and third group were injected subperiosteally on the palate with $PGE_2$ $10{\mu}g$ and evening primrose oil 10mg respectively. The fourth group was given indomethacin $20{\mu}g/m{\ell}$ orally by water bottle. The maxillary first molar was moved mesially from the incisors using a 50gm force rubber band. In each group at the 1, 2, 3, 5, and 7th day, 4 rats were examined by light microscope, and 4 by electron microscope. The obtained results were as follows: 1. Osteoclastic activity was maximum at the 3rd day in the $PGE_2$ group on the interradicular alveolar bone of the first molar, followed by the evening primrose oil group, control group, and indomethacin group. 2. Root resorption and vacuolar changes were maximum in the $PGE_2$ group. 3. At the 3rd day of the $PGE_2$ group, the osteoclasts showed well developed ruffled borders and clear zones. At the same day, the evening primrose oil group also showed well developed ruffled borders and clear zones, but less than the $PGE_2$ group. 4. At the 3rd and 5th day of the $PGE_2$ group, fibroblasts showed phagocytized fragmented collagen fibers in the cytoplasm. At the 7th day of the $PGE_2$ group, fibroblasts showed collagen fibers forming at the cell membrane surface.
이원유,최성호,김성희,Lee, Won-You,Choi, Seong-Ho,Kim, Sung-Hee 대한치주과학회 1994 Journal of Periodontal & Implant Science Vol.24 No.2
This study investigates the effect of wind instrument playing on the periodontal condition by comparing and analyzing wind instrument players and non-wind instrument players by various clinical paramenters, including periodontal index, bleeding index, simplified oral hygiene index, and non-functional tooth index. The subjects consist of 65 wind instrument players and 31 non-players. The players were divided into 4 groups ; Those who use cup-shaped mouthpieces as Group A, single reed mouthpieces as Group B, double reed mouthieces as Group C, and hole mouthpieces as Group D. Non-players are designated as Group E. The results are as follows ; 1. Periodontal Index Group A recorded $1.2{\pm}0.7$, Group B $1.1{\pm}0.7$, Group C $1.3{\pm}0.5$, Group D $2.1{\pm}0.5$, and Group E $0.9{\pm}0.6$, with no statistically significant differences among the groups. 2. Bleeding Index Group A shwed 0.23, Group B 0.25, Group C 3.0, Group D 0.38, and Group E 0.23, with no statistically significant differences among the groups. 3. Non-functional Tooth Index Group A recorded 2.0, Group B 1.0, Group C 3.0, Group D 0, Group E 0.7, with no statistically significant differences among the groups. 4. Simplified Oral Hygiene Index Buccal sides of maxillary 1st molars, lingual sides of mandibular 1st molars, labial sides of maxillary anterior teeth, and lingual sides of mandibular anterior teeth were investigated, and there was no statistically significant differences among the groups.