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플루란 아세테이트 미세구의 제조와 Lidocaine의 방출
나건,김성민,이기영 全南大學校 觸媒硏究所 1998 觸媒硏究 論文集 Vol.20 No.-
Release of lidocaine using pullulan was studied Hydrophobic pullulan acetate was prepared by chemical modification of pullulan and pullulan acetate microsphere was prepared by solvent evaporation method. The size of microsphere was 1 - 2 μm and the drug loading efficiencies of microspheres were approximately 17.8% and 26.6% at the agitation speed of 8000 rpm and 13500 rpm, respectively. The microspheres prepared at variable stirring speed showed variable microsphere size. Lidocaine was released more than 60% in 4 hrs fastly and more than 90% in 12hrs.
신은경,김동운,김유은,나건,이기영 全南大學校 觸媒硏究所 1998 觸媒硏究 論文集 Vol.20 No.-
In this study, we prepared biodegradable polysaccharide films with pullulan, κ-carrageenan, curdlan and chitosan and measured physical properties and antibacterial activity All of polysaccharide films showed the high biodegradability but antibacterial activity on bacteria was only observed at chitosan film. The permeability of the gas(O₂and C0₂) of polysaccharide films besides chitosan film showed 50∼100 times lower than polyethylene(PE) film. Therefore, we prepared two film types to give low gas permeability and antibacterial property at polysaccharide film : one type was the physically mixed film(k-carrageenan +chitosan) and other type was the chitosan coated κ-carrageenan film. Chitosan coated κ-carrageenan film showed low gas permeability and antibacterial activity.
Rahnella aquatilis를 이용한 Lactan gum 생산에서 탄수화물 대사
나건,이성호,이기영 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4
Rahnella aquatilis에 의해 생산되는 lactan gum은 고점성 물질로 의가소성 유동거동을 보인다. pH가 7.0으로 제어된 발효조 배양에서 lactose나 sucrose같은 2당을 기질로 삼았을 때의 lactan 생산량은 glucose나 galactose같은 단당의 경우보다 2배 이상의 큰 값을 보였다. 초기탄소원 농도가 45 g/l인 lactose와 sucrose의 lactan gum 생산량은 27, 28g/l로 60% 이상의 수율을 보였다. Lactose를 glucose와 galactose로 가수분해시키는 β-galactosidase의 활성은 lactose와 galactose를 기질로 사용하였을 때 유도되었고 lactose를 기질로 사용한 경우 최고 4000U/ml까지의 큰 값을 보였다. 그러나 초기 탄소원이 혼합 탄소원 I (glucose+galactose)인 경우 단당(glucose, galactose)의 경우에 비해 lactan gum 수율이 높았고 45 g/l의 lactose와 비슷하였다. β-galactosidase의 반응이 필요하지 않은 혼합탄소원 I의 경우에서 lactose로의 lactan gum 생산능과 비슷한 값을 보인 것으로 보아 β-galactosidase에 의한 lactose 분해반응은 율속 단계가 아닌 것으로 판단된다. 초기 탄소원을 혼합 탄소원 Ⅱ(glucose+ fructose)으로 하였을 때 총 탄소원의 소비속도는 sucrose보다 늦었으며 glucose의 소비가 fructose의 소비보다 빨랐다. Lactan gum produced by Rahnella aquatilis is a high viscous, anionic polysaccharide and has shear thinning behaviour. Lactan gum yield and concentration was greater on disaccharides such as lactose and sucrose than on monosaccharides such as glucose and galactose. When initial carbon source concentration was 45 g/l sucrose or lactose, the microorganisms produced 26 g/l and 27 g/l of lactan, respectively with a yield more than 60%. β-Galactosidase, hydrolying lactose into galactose and glucose, was induced by lactose or galactose. When initial carbon source was 45 g/l of mixed carbon I (glucose:galactose=1:1), lactan gum concentration was higher than that from 45 g/l of monosaccharide (glucose or galactose) but was similar to the result from 45 g/l of lactose. Therefore, lactose hydrolysis reaction by β-galactosidase does not seem to be rate determining step in lactan gum biosynthesis. When initial carbon source was 45 g/l of mixed carbon Ⅱ (glucose:fructose=1:1), total carbon source consumption rate was slower than that from sucrose, but glucose consumption rate was faster than that from fructose.