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유대열 한국유가공기술과학회 1997 Journal of Dairy Science and Biotechnology (JMSB) Vol.15 No.2
Lactoferrin is an 80 kDa, iron-binding glycoprotein present in milk and, to a lesser extent, in exocrine fluids such as bile and tears. It consists of a single-chain polypeptide with two globular lobes and is relatively resistant to proteolysis. Owing to its iron-binding properties, lactoferrin has been proposed to play a role in iron uptake by the intestinal mucosa and to act as a bacteriostatic agent by withholding iron from iron-requiring bacteria. Beside this, the functions proposed for lactoferrin are diverse and include immunomodulatory activity, regulation of myelopoiesis, cell growth promotion and differentiation, and antioxidant effects. Therefore lactoferrin has been a good target for commercial production. Until now lactoferrin has been purified from human, bovine, sheep, goat and horse milk. Complete amino acid sequences of the lactoferrin from human, murine, bovine, porcine, and caprine have been determined, either directly at the protein level or deduced from the nucleotide sequence and shown to display a high level of similarity. We, researchers in my laboratory, have been studied lactoferrin for mass production by using the techniques of transgenic animal, transgenic plant and microorganism. Here we present the data on structure, function and production of lactoferrin.
유대열 한국실험동물학회 2020 Laboratory Animal Research Vol.36 No.1
The hepatitis B virus (HBV) infects approximately 240 million people worldwide, causing chronic liver disease (CLD) and liver cancer. Although numerous studies have been performed to date, unfortunately there is no conclusive drug or treatment for HBV induced liver disease. The hepatitis B virus X (HBx) is considered a key player in inducing CLD and hepatocellular carcinoma (HCC). We generated transgenic (Tg) mice expressing HBx protein, inducing HCC at the age of 11–18 months. The incidence of histological phenotype, including liver tumor, differed depending on the genetic background of HBx Tg mice. Fatty change and tumor generation were observed much earlier in livers of HBx Tg hybrid (C57BL/6 and CBA) (HBx-Tg hybrid) mice than in HBx Tg C57BL/6 (HBx-Tg B6) mice. Inflammation was also enhanced in the HBx-Tg B6 mice as compared to HBx-Tg hybrid mice. HBx may be involved in inducing and promoting hepatic steatosis, glycemia, hepatic fibrosis, and liver cancer. Reactive oxygen species (ROS) generation was remarkably increased in livers of HBx Tg young mice compared to young wild type control mice. Previous studies on HBx Tg mice indicate that the HBx-induced ROS plays a role in inducing and promoting CLD and HCC.
유대열,이경광 中央大學校 遺傳工學硏究所 1993 遺傳工學硏究論集 Vol.6 No.1
In 1980's transgenic animals expressing growth hormone genes derived from various species were developed to improved growth of livestocks or feed efficiency. But they resulted in many side effects because the constitutive and over expression of the genes affected the endocrine system. To solve these problems, other approaches using peptides related with muscle differentiation were tried but it has so far been unsuccessful. Recently, transgenic technology has been applied to develop livestocks which secrete large amounts of foreign proteins into mammary glands. Animal bioreactor is one of the most promising approaches applicable to industry. Although some transgenic animals show side effects like lactational shutdown, the animal bioreactor system is hopeful if we develop efficient expression vectors and get the basic information for the item to be produced. Basic knowledge and various technologies, including gene cloning, recombinant DNA, in vitro cell culture micromanupulation of embryos, cryopreservation, embryo transfer, breeding of animals, should be put together on line to improve the productivity of transgenic animals.