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Brucella 면역혈청이 균생존과 호흡에 미치는 영향
안태휴 대한미생물학회 1970 大韓微生物學會誌 Vol.5 No.1
The survival rate of Brucella in the immune and normal serum was compared with the respiration rate of cells in the presence of the serum. The Brucella were suspended in the serum of original concentration and incubated at 37 C for 3 hr. Then, the cells that survived were counted by the routine pour plate method. As to the measurement of bacterial oxygen uptake the standard manometrie techniques were applied. Contrasting results were observed between the serum bactericidal activity and the respiration of Brucella. The bactericidal index of Brucella immune serum was 1.4 to 1.6 while 2. 7 at normal serum, the difference being more than one log10 unit, whereas the oxygen uptake rate of Brucella in their homologous immune serum was less than a half of the rate in no seurn. Immune bodies exerted the inhibitory effect to-gether with complement on the oxidative metabolism of Brucella Addition of various substrates such as D-alanine, L-glutamic acid, and glucose, into the Warburg flask did not bring about any noticeable change in the effect of immune and normal serum on the bacterial metabolism.