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최진웅 ( Choi Jin-woong ),심재중 ( Shim Jae-jung ),박준명 ( Park Jun-myung ),홍성남 ( Hong Sung-nam ),박선규 ( Park Sun-kyu ) 한국구조물진단유지관리공학회 2010 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.14 No.1
The object of this study is to estimate shear strength of Perfobond Rib shear connectors were used by direct shear stress. To estimate shear strength, Perfobond Rib shear connector experiments were fabricated with four variables which are existence or nonexistence hole, number of holes, distance of hole and number of reinforcing bars and Push-out Tests were carried out on them. After experiments, mechanism of Perfobond Rib shear connector was examined. The result of experiments, primary factors which influence direct shear strength are point bearing capacity of Perfobond Rib shear connector, effect of concrete dowels and effect of reinforcing bars. Lastly, based on primary facors, direct shear strength formula was proposed.
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Bacillus subtilis Y3-7 배양액의 α-glucosidase 활성 억제 효과
명길선(Kil-Sun Myoung),허건(Gun Heo),윤석영(Suck-Yong Yoon),심재중(Jae-Jung Shim),이정희(Jung-Hee Lee),임광세(Kwang-Sei Lim),허철성(Chul-Sung Huh) 한국식품과학회 2008 한국식품과학회지 Vol.40 No.5
본 연구에서는 전통 발효 식품에서 분리한 B. subtilis Y3-7 균주의 배양액이 α-glucosidase의 활성에 미치는 영향을 살펴보았다. B. subtilis Y3-7 균주를 TSB 배지에 30시간 이상 배양한 배양액은 1.62 ㎎/mL의 농도로 yeast α-glucosidase의 활성을 50%까지 저해할 수 있었다. 또한 효소학적 분석을 통해 이것이 배양액 내 물질이 효소의 기질과 경쟁적으로 작용하여 억제효과가 나타남을 확인하였다. B. subtilis Y3-7 균주의 배양액은 yeast 뿐만 아니라 실험동물의 장내 α-glucosidase의 활성에도 영향을 미쳐 당부하에 따른 급격한 혈당상승을 억제할 수 있었다. 이와 같은 결과로 보아 대량배양이 가능한 B. subtilis Y3-7을 통해 탄수화물 흡수에 중요한 역할을 하는 α-glucosidase 활성억제 물질을 얻음으로써 항당뇨 물질의 안정적인 생산이 기대되는 바이다. Culture broth of Bacillus subtilis Y3-7 in tryptic soy broth (TSB) isolated from Korean traditional fermented food was evaluated for the inhibition of α-glucosidase. The results of in vitro studies using the yeast α-glucosidase demonstrated that the culture broth exerted inhibitory effects on α-glucosidase with IC?? value of 1.62 ㎎/mL, and functioned as a competitive inhibitor. Furthermore, the culture broth of B. subtilis Y3-7 significantly improved glucose tolerance in normal and streptozotocin-induced diabetic mice. The blood glucose levels in the mice receiving sucrose supplementation in the culture broth (1 g/㎏, 2 g/㎏) were measured at 48.7%, which corresponded to 22.2% of the levels measured in the control mice. These results indicated that the culture broth of B. subtilis Y3-7 in TSB might be considered as a useful compound for the preparation of functional foods for diabetic patients.
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BALB/c 마우스에서 발효 홍삼 Ginsenoside의 생체이용율과 항염효과
이은규(Eun Kyu Lee),배주현(Chu Hyun Bae),김유진(Yu Jin Kim),박수동(Soo-Dong Park),심재중(Jae-Jung Shim),유영법(Youngbob Yu),이정열(Jung-Lyoul Lee) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.5
The fermented red ginseng by microorganism is known to increase pharmacological activity in vivo. To evaluate the bioavailablity of red ginseng fermented by probiotics, we conducted the pharmacokinetic study of ginsenoside Rb1, Rd and total ginsenosides (TG, ginsenosides Rb1 + Rd + Rg1 + F2 + Rg3 + compound K) in BALB/C mice. The AUC value of ginsenoside Rb1 in mice serum administered with 600㎎/㎏ drugs showed 21.93 ± 14.68 ng·h/mL (RGw, water extract), 275.211 ± 110.04 ng·h/mL (RGe, 50% ethanol extract) and 404.91 ± 162.57 ng·h/mL (fRGe, fermented red ginseng extract). Analysis of ginsenoside Rd also showed a higher ACU value in fRGe than in RGw or RGe. And the AUC value of total ginsenosides in mice serum treated with 600 ㎎/㎏ were observed 42.12 ± 23.44 ng·h/mL (RGw), 321.44 ± 133.5 ng·h/mL (RGe) and 537.33 ± 229.01 ng·h/mL (fRGe), respectively. Cmax value of ginsenoside Rb1 in mice administered with 600㎎/㎏ were observed 3.67 ± 3.34 ng/mL (RGw), 23.27 ± 8.81 ng/mL (RGe) and 25.52 ± 7.29 ng/mL (fRGe). These results can be considered that the fermented red ginseng has more bioavailability than that of unfermented red ginseng. In quantitative analysis of the inflammation-related cytokines IL-1β and TNF, no significant difference was found between the fermented red ginseng (fRGe) and the red ginseng (RGe).
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