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송길호,박해두,진철제,신성갑,Song, Gil-Ho,Park, Hae-Du,Jin, Cheol-Je,Sin, Seong-Gap 대한기계학회 1998 大韓機械學會論文集A Vol.22 No.4
With the introduction of edge drop control system in Tandem Cold Rolling Mill, it is necessary to develop te numerical expression for the set-up and edge drop automatic control of cold rolled sheet. As a first step we developed a simulation program which predicts profile and the amounts of edge drop at the delivery side of each stand by using roll deformation anlysis with the slit roll model. And by using the program the effect of various rolling conditions on edge drop was investigated. As a result the relations were obtained between the amounts of edge drop and rolling conditions. Based on above relations, the numerical expression was developed for the set-up and automatic control of edge drop by multi-regression of simulation results for the variation of edge drop amount with each rolling condition.
하이브리도마 방법에 의한 항 α - Proteinase Inhibitor 모노클론항체의 생산과 분석
강신성,방옥선,강희갑,손종경 ( Shin Sung Kang,Ok Sun Bang,Hee Kap Kang,Jong Kyung Sonn ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.4
Monoclonal antibodies (MAbs) against human α₁-PI was produced by hybridizing SP 2/0 -Ag 14 mouse myeloma cells with spleen cells of Balb/c mice immunized with a α₁-PI. The resulting hybridoma clones were screened for their ability to bind α₁-PI by ELISA. Seven positive stable monoclones were obtained by cloning three times in serial dilutions, and each antibody was identified to be homogeneous by several criterial. By western bloting analysis using CNBr-cleavage peptide fragments (Fr I: amino acid residues 64-220; Fr II: 243-351; Fr III : 1-63), four of these monoclones were found to synthesize MAb specific to Fr I, and one clone to Fr II. But MAbs of the other two clones showed reactivity only to intact α₁-PI. Five of our monoclones were appeared to synthesize IgG₁(k) antibodies having affinity constant in the range 0.1-1.1×10^9 M^(-1)
사람 alpha-fetoprotein 에 대한 단일클론 항체의 생산 및 분석
강신성,강희갑,박대규 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
Monoclonal antibodies (MAbs) against human alpha-fetoprotein (AFP) was produced by hybridizing SP 2/0-Ag 14 mouse myeloma cells with spleen cells of Balb/c mice immunized with purified AFP Two subclones (D-6 and E-6) were expanded as ascite tumors in syngenic mice, and from ascitic fluid immunoglbulins were pruified. Each antibody was identified to be homogeneous by several criteria, and the affinity constant of D-6 and E-6 MAb to AFP was calculated to be 4.2×10^(-8) and 6.4×10^(-8) M^(-1), respectively. With these MAbs sensitive and accurate enzyme linked immunosorbent assay mothod was established.
강신성,방옥선,강희갑,손종경,Kang, Shin-Sung,Bang, Ok-Sun,Kang, Hee-Kap,Sonn, Jong-Kyung 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.4
Alpha-1-proteinase inhibitor(${\alpha}_1-PI$)로 면역시킨 Balb/c 마우스 비장세포와 SP 2/0-Ag 14 마우스 미에로마 세포를 융합시키는 하이브리도마 기법으로 모노클론 항체 생산을 실시하였다. 하이브리드클론들이 생산하는 항체들과 ${\alpha}_1-PI$와의 반응성은 ELISA로 분석하였으며, 양성클론을 제한희석법으로 클로닝하여 7클론의 항-${\alpha}_1-PI$ 특이 클론세포를 얻었고, 이들의 단일클론성을 확인하였다. 7클론 중 5클론이 생산하는 모노클론항체는 항원에 대한 결합상수가 $0.1-1.1{\times}10^9M^{-1}$의 $IgG_1(k$) 이었고, 이들 항체의 항원 특이성을 ${\alpha}_1-PI$의 CNBr-peptide 분획을 이용한 Western blot를 실시한 결과 FrI(아미노산 잔기 64-220)에 특이한 것이 4클론, Fr II (243-351)에 특이한 것이 1클론이었고, 나머지 2클론의 특이성은 결정할 수 없었다. Monoclonal antibodies (MAbs) against human ${\alpha}_1-PI$ was produced by hybridizing SP 2/0-Ag 14 mouse myeloma cells with spleen cells of Balb/c mice immunized with ${\alpha}_1-PI$. The resulting hybridoma clones were screened for their ability to bind ${\alpha}_1-PI$ by ELISA. Seven positive stable monoclones were obtained by cloning three times in serial dilutions, and each antibody was identified to be homogeneous by several criterial By western bloting analysis using CNBr-cleavage peptide fragments (Fr I: amino acid residues 64-220; Fr II: 243-351; Fr III: 1-63), four of these monoclones were found to synthesize MAb specific to Fr I, and one clone to Fr II. But MAbs of the other two clones showed reactivity only to intact $a_1$-PI. Five of our monoclones were appeared to synthesize $IgG_1(k$) antibodies having affinity constant in the range $0.1-1.1{\times}10^9M^{-1}$.
단핵구 분화에 있어서 fibronectin 수용체의 역할
강신성,방옥선,강희갑,이영섭,박의균 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
The interaction between flbronectin (FN) and its receptor controls cell attachment and migration, two crucial events during monocyte development and differentiation. To investigate the functional role of FN and its receptor, we have studied adhesion of monocyte to two different regions of FN (38- and 85-kDa domain), as well as the expression of the integrin during monocyte differentiation. Anti-integrin β1 subunit antibody completely blocked the attachment of FN-coated latex beads to macrophage, but the effect of anti-integrin α4 antibody was much less significant. Rat monocyte expressed integrin α4β1 predominantly, while macrophage pressed a5f31 as analyzed by flow cytometer and western blot. From these results, it can be suggested that these two integrins play different role: during monocyte differentiation.
태반형 알칼라인 포스파타아제 유전형에 대한 단일클론 항체
강신성,강희갑,윤종희,방옥순,이영섭 한국유전학회 1993 Genes & Genomics Vol.15 No.4
A total of nine monoclonal antibodies (MAbs) against common phonotypes of human placental alkaline phosphatase (PLAP) was produced by hybridoma using a mixture of PLAP subtypes (type 1+2+3) as antigen. Six of these reacted only with PLAP, but not with intestinal and liver alkaline phosphatases. Three out of these six MAbs have been found to discriminate at least one of the product of three common alleles from others, while the other three MAbs reacted with the six common allelic varients. The results suggest that anti-PLAP MAbs can be used as probes to discriminate among allelic varients of PLAP.
항-AFP 단일클론 항체를 이용한 간암진단 효과의 검토
현광자,강신성,강희갑 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
To check the possible application of our anti-AFP monoclonal antibodies (MAbs) as immunodiagnostic reagents for hepatocellular carcinoma, ELISA and immunohistochemical assay were performed on the sera and liver biopsy specimens from the patients of hepatocellular carcinoma and other non-malignant hepatic disease. By non-competitive ELISA using anti-AFP MAbs, the highest incidence of AFP value was found only in the sera of hepatocellular carcinoma patients, i.e., more than 54% of patients had serum AFP levels of more than 500 ng/㎖. By immunoperoxidase and indirect immunofluorescence techniques, anti-AFP MAbs were found to react with cytoplasm of hepatocellular carcinoma cells. However immunohistochemical reactivity to AFP in hepatocellular carcinoma cells was lower than that in non-neoplastic liver cells adjacent to the hepatocellular carcinoma. From these results with the similar findings from other studies, we suggest that AFP antigen is appropriate in the diagnosis assay (ELISA) but is not by immunohistochemical detection.