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RISS 인기검색어
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- 저자 : 신광순(Kwang - Soon Shin) (검색결과 98 건)
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식물성 식품재료로부터 보체계 활성화 다당의 검색 및 그 활성검토
신광순,라경수,성하진,양한철,Shin, Kwang-Soon,Ra, Kyung-Soo,Sung, Ha-Chin,Yang, Han-Chul 한국식품과학회 1993 한국식품과학회지 Vol.25 No.3
식물성 식품재료를 대상으로 보체 용혈분석법$(TCH_{50})$ 을 이용하여 보체 활성화능(항보체 활성)에 대한 검색을 실시하였다. 총 38종의 식용 식물추출물 중 5종의 시료에서 대조구에 비해 60% 이상의 $TCH_{50}$ 감소를 일으키는 비교적 높은 활성을 나타냈으며 그 활성의 순서는 $1000{\mu}g/ml$ 시료 농도에서 생강>토란대>냉이>은행잎>달래이었다. 한편 가장 높은 활성을 보였던 생강에서 조제된 ZR-1의 경우 pronase 소화 후에는 활성의 변화가 없는 반면에 과요오드산 산화에 의해서는 급격한 활성의 감소를 나타냄으로써 ZR-1의 단백질 부위가 아닌 다당 부위가 활성에 기여함을 알 수 있었다. 또한 ZR-1의 항보체 활성은 $Ca^{2+}$ 이온 부재시 부분적인 감소현상을 보였으며, ZR-1을 정상인의 혈청과 반응 후 anti-humanC3를 이용하여 2차원 면역 전기영동을 행한 결과 C3의 분해산물을 관찰할 수 있었다. 또한 이들 획분은 $ACH_{50}$의 저해를 일으켰다. 동 결과로부터 ZR-1의 보체 활성화 양식은 classical pathway 뿐만 아니라 alternative pathway도 경유함을 알 수 있었다. Screenings were performed on edible plants to examine their complement-system activating ability (anti-complementary activity) by hemolytic complement assay $(TCH_{50})$. Among 38 kinds of plant extracts, 5 kinds showed relatively strong anti-complementary activity which decreased $TCH_{50}$ more than 60% comparison with control and the order of activity was Zingiber officinale>Colocasia antiquorum>Capsella bursapastoris>Ginkgo biloba>Alium monanthum in $1000{\mu}g/ml$. The anti-complementary activity of ZR-1 prepared from the root of Zingiber officinale which was showed the most potent activity, did not change by pronase treatment, but decreased greatly by periodate oxidation. These results indicate that not protein moiety but carbohydrate moiety in ZR-1 fraction may also contribute to the anti-complementary activity. Also, the anti-complementary activity of ZR-1 was reduced partially in the absence of the $Ca^{2+}$ ion. When crossed immunoelectrophoresis using anti-human C3 serum was carried out after incubation of normal human serum with the ZR-1 in $Ca^{2+}$ free condition, a cleavage of C3 precipitin line was observed. Furthermore this polysaccharide fraction considerably inhibited $ACH_{50}$. These results also indicate that the mode of complement activation by polysaccharide from Zingiber officinale is via not only the classical pathway but also the alternative pathway.
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B형간염(型肝炎) 표면항원(表面抗原)의 주면역원(主免疫原) 결정기(決定基)에 특이(特異)한 합성(合成) Peptide의 면역원성(免疫原性)에 관한 연구(硏究)
신광순,한수남,Shin, Kwang-soon,Han, Su-nam 대한수의학회 1985 大韓獸醫學會誌 Vol.25 No.1
Many investigators have been pursuing various attempts so far to produce hepatitis B surface antigen(HBsAg) vaccines using the techniques such as isolation from plasma of chronic HBsAg carrier, recombinant DNA technique or preparation of synthetic peptides specific for immunogenic determinants. Hepatitis B virus can not grow on any cell lines by the tissue culture technique at the present time. The plasma of chronic HBsAg carrier is expensive and its source is limited. The HBsAg from the recombinant DNA technique gave still very low yield. Another approach, therefore, has been initiated to develop a synthetic hepatitis B virus vaccine. The possible use of several distinct synthetic vaccines in prophylaxis can be facilitated by availability of full synthetic immunogens. Peptides synthesized for potential application as antiviral vaccines have been mostly tested in the form of conjugates with carrier proteins, although the free synthetic peptide can be immunogenic. To understand basic knowledges on the antigenicity and immunogenicity of a synthetic peptide specific for major immunogenic determinant of HBsAg, a nonapeptide, $H_2N^{139}Cys-Thr-Lys-Pro-Thr-Asp-Gly-^{146}Asn-Aba$ COOH, which corresponds to HBsAg amino acid residues 139 to 147, was synthesized by the Merrifield's solid-phase method with a slight modification. The antigenicity and immunogenicity of this specific synthetic peptide were examined comparing with purified plasma-derived natural HBsAg. The results obtained are as follows; 1. The peptide synthesized showed the identical amino acid composition to the theoretical value. The degree of purification and molecular weight were acertained by methods of high performance liquid chromatography and mass spectrometry. 2. Using m-maleimidobenzoyl-N-hydroxysuccinimide ester as a conjugating agent, the synthetic peptide was conjugated to rabbit albumin and ${\gamma}$-globulin, tetanus and diphtheria toxoids, and keyhole limpet hemocyanin. Their conjugation yields were 8.3, 9.5, 15.8, 13.5, and 11.2%, respectively. 3. The natural HBsAg was purified from plasma of chronic HBsAg carrier. By the electron microscopic observation of the purified natural HBsAg preparation, no Dane particles were observed and the preparation showed negative DNA polymerase activity. 4. Antigenicity of the synthetic peptide and the plasma-derived natural HBsAg was determined by competition radioimmunoassay using $^{125}I$-natural HBsAg. Their 50% inhibitions appeared as $90{\mu}g/ml$ and $0.12{\mu}g/ml$ for the synthetic peptide and the natural HBsAg, respectively. This indicates that the former was about 750-fold less antigenic than the latter. 5. Immunogenicity of the synthetic peptide was determined by administering the peptide-carrier conjugates into rabbits with and without Freund's complete adjuvant. Regardless the carrier proteins and adjuvant, positive immune responses to the synthetic peptide were observed. The higher antibody titers, however, were shown in the groups administered with Freund's complete adjuvant. 6. Immunizing dose 50% in mice of the various peptide-carrier conjugates was 5.47, 6.00, 65.16, 31.25 and $13.03{\mu}g/dose$ for rabbit albumin and ${\gamma}$-globulin, tetanus and diphtheria toxoids, and keyhole limpet hemocyanin, respectively, while the natural HBsAg showed $0.65{\mu}g/dose$. 7. It was postulated that homologous proteins prefer to heterologous ones as the carriers.
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경주마(競走馬) 혈청(血淸)의 단백질(蛋白質), Bilirubin, Sodium 및 Potassium 농도(濃度)와 Thymol Turbidity
신광순,나동진,문희주,조종후,Shin, Kwang Soon,Rha, Dong Jin,Moon, Hi Joo,Cho, Jong Hoo 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.1
Concentration of total protein, bilirubin, sodium and potassium in blood sera, and thymol turbidity of race horses, 78 males and 57 females aging from 2 to 5 years, were estimated. The results obtained were as follows: 1. Serum of race horses was low in protein concentrations as mean values of $5.48{\pm}0.77$ g/100ml. 2. Serum bilirubin was in normal range as mean values of total bilirubin, direct bilirubin and indirect bilirubin were $1.05{\pm}0.57$, $0.12{\pm}0.05$ and $0.93{\pm}0.58$ mg/100ml, respectively. 3. Mean value of serum thymol turbidity was $1.41{\pm}0.72$ S-H unit. 4. Mean values of serum sodium and potassium of race horses from Australia were $143{\pm}8.7$ and $3.85{\pm}0.53$ mEq/liter, respectively, and mean values of serum sodium and patassium of race horses from Japan were $142{\pm}9.4$ and $3.58{\pm}0.60$ mEq/liter, respectively.
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Kwang Soon Shin(申光淳),Jonghoo Cho(趙鍾厚),Sok Namkung(南宮錫),Dae Woo Whang(黃大羽) 한국예방수의학회 1979 예방수의학회지 Vol.3 No.1
Residual nitrite content of ham and sausage during storage for various lengths of time were estimated. The results obtained were summerized as follows. 1. Residual nitrite levels of ham and sausage during refrigerated storage(storage temperature 5℃) were rapidly decreased by first storage day 8 but showed a significant increase by storage day 20 again and these products maintained these increased low levels for about 1 week. After storage day 26 all products showed decreasing residual nitrite levels by storage day 30. 2. Ham and sausage showed more rapid rates of decrease in residual nitrite during room temperature storage (30℃) than refrigerated storage, especially during the first 4 days of storage, and then ham had these low levels of residual nitrite for about 20 days of storage while sausage maintained these low levels for about 10 days. After storage days of these low levels of residual nitrite all products tested showed the trends of gradually increasing residual nitrite by the last of storage period 30 days.
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kwang soon Shin(申光淳) 한국예방수의학회 1978 예방수의학회지 Vol.2 No.1
Ice cream is made from milk by concentrating it. It also well known to be mutritious and regarded as a good taste food and we can easily obtain it out of season. The chief ingredient of ice cream is milk fat, which is easily digested in comparison with vegetable fat and contributes to nutrition value and quality of the products. For the study I selected samples of icecream by package type which are in brisk demand from the big makers and measured each total lipid contents and acid, saponification and incline values. After this, I analyzed their fatty acid composition by Gas-liquid chromatography. The results obtained are as follows, 1) Average total liquid contents of samples (milk, carton, cone and bar type ice cream) were 3.0 4%, 8.4%, 6.4% and 5.4% respectively. 2) Average acid value of samples (milk, carton, cone and bar type ice cream) ranged differently between 180 and 260 by the makers and average saponification value were 208, 226, 226, and 215 respectively. 3) The incline value of samples (milk, carton, cone and bar type ice cream) ranged from 10 to 32, and showed wide variety by the makers and average iodine value were 29, 20, 19 and 22 respectively, 4) The milk and ice cream analyzed contained seventeen kinds of fatty aicd. Among those contained in milk, palmitic acid was foremost by 29.8%, followed by oleic acid 27.1%. It seems, however, that the average of the fatty acid analyzed by the package type is not meaningful for those contained in ice cream varied widely by the makers. Main components were found to be from butyric acid to linolenic acid and were showed in figures 5, 6, 7, 8, 9 and Table 6. The most outstanding feature of fatty acid composition of ice cream was that it was almost the same by samples but different widely by the makers. Another feature that the ice cream contained more !auric acid but less oleic acid was than milk fat except for the ice cream products of one maker.
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