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인삼 사포닌의 생화학적 연구 ( ⅩⅩⅤ ) : 인삼 사포닌 분획이 대두 발아시 Ascorbic acid 생합성에 미치는 영향
배수경,곽한식,주충노 ( Soo Kyung Bae,Hahn Shik Kwak,Chung No Joo ) 생화학분자생물학회 1986 BMB Reports Vol.19 No.1
It was found that ascorbate synthesized during the germination of Glycine max was always much higher than that of control when the soybeans were rinsed in 10-4% ginseng saponin solution at first for 24 hours and the same solution was poured afterwards. Using the homogenate of germinating soybean (2nd day) as enzyme source and glucose containing [U-^(14)C]-glucose as substrate, it was confirmed that the saponin fraction stimulated the ascorbate biosynthesis during germination. From the radioactivity distribution of the intermediates from glucose to ascorbate. it was realized that ascorbate was synthesized from glucose predominantly via galacturonate pathway rather than glucuronate pathway indicating that the glucose was epimerized first to galactose by the action of UDP-glucose epimerase.
인삼 사포닌의 생화학적 연구 (XXV) : 인삼 사포닌 분획이 대두 발아시의 Ascorbic acid 생합성에 미치는 영향
배수경,곽한식,주충노,Bae, Soo-Kyung,Kwak, Hahn-Shik,Joo, Chung-No 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.1
ascorbate 합성이 대조군에 비해 촉진됨을 확인하였다. 대두 발아시의 ascorbate 합성 경로와 이에 미치는 인삼 사포닌의 영향을 조사하기 위해 발아 2일째의 대두를 효소원으로 하고 [U-$^{14}C$]-glucose를 포함한 glucose를 기질로 하여 대조 반응액과 인삼 사포닌($10^{-4}%$)이 포함된 시험 반응액에서 각각 반응시킨 후 ascorbate 합성 중간물질의 방사능 분포를 조사한 결과 ascorbate의 합성은 glucuronate 경로 보다는 주로 galacturonate 경로로 생성됨을 알 수 있었고, 인삼 사포닌($10^{-4}%$)이 함유된 시험 반응액에서의 중간물질의 방사능이 대조군보다 언제나 높았다. 또한 UDP-glucose epimerase에 미치는 인삼 사포닌의 영향을 조사한 결과 반응액에서의 인삼 사포닌의 농도가 $10^{-4}%$일 때 효소 활성(대조군의 1.44배)이 가장 컸으며 $10^{-5}%$일 때도 효소 활성이 크게 (대조군의 1.38배) 증가하였다. It was found that ascorbate synthesized during the germination of Glycine max was always much higher than that of control when the soybeans were rinsed in $10^{-4}%$ ginseng saponin solution at first for 24 hours and the same solution was poured afterwards. Using the homogenate of germinating soybean (2nd day) as enzyme source and glucose containing [U-$^{14}C$]-glucose as substrate, it was confirmed that the saponin fraction stimulated the ascorbate biosynthesis during germination. From the radioactivity distribution of the intermediates from glucose to ascorbate, it was realized that ascorbate was synthesized from glucose predominantly via galacturonate pathway rather than glucuronate pathway indicating that the glucose was epimerized first to galactose by the action of UDP-glucose epimerase.
배수경(Bae, Soo-Kyung),공순구(Kong, Soon-Ku) 한국실내디자인학회 2015 한국실내디자인학회 학술대회논문집 Vol.2015 No.10
Museums were initially a place for preservation of documents or precious artifacts, and the second generation concept of museums evolved into exhibition places for the general public, and in modern times, museums have transformed into places of cultural exchange for engaging communication with museum visitors. Korean Christianity fulfilled a crucial role in the modernization of Korea, and by sending missionaries to places all over the globe, Korean Christianity is spreading the religious and cultural values of Korea. While the role that Christian museums serve by collecting, examining, researching and displaying Christianity related artifacts is significant, the reality is that due to a lack of awareness of the importance of museums and due to other difficult circumstances, compared to their significance there is a lack in the diversity and professionalism regarding museum exhibition contents and production. This research study examines the current state of Christian museums, and through advanced case studies both in Korea and abroad, this research is significant in creating new exhibition spaces that engage the audience of museums and communicate with the audience through production elements that are relevant to the times and exhibition type.
배수경(Soo-Kyung Bae),이영철(Young-Chul Lee),김현위(Hyeon-Wee Kim) 한국식품영양과학회 2001 한국식품영양과학회지 Vol.30 No.1
사과농축액에서 일어나는 갈변반응을 억제하고자 갈변억제제 즉, PVPP(polyvinypolypyrrolidone)와 A.A.(ascorbic acid)를 첨가한 후 갈변의 원인으로 추정되는 여러 요인들의 변화를 살펴보았다. 저장기간에 따른 색차값 및 갈변도 변화에서 PVPP 첨가농축액의 L값 및 ΔE값, 흡광도값에서 대조구에 비해 갈변저해 정도가 뚜렷함을 알 수 있었다. 사과농축액(대조구)의 변화에서는 저장기간이 경과할수록 sucrose의 감소, glucose와 fructose의 증가 등 유리당의 변화와 아미노산 함량의 감소, HMF(hydroxymethylfurfural) 함량의 경우 저장초기(2.06 mg/100 g)와 저장 90일째(30.61 mg/100 g)의 차이, A.A. 함량의 감소 정도가 큰 농축액에서 갈변이 심화되는 등 비효소적 갈변의 지표들이 변화함을 보이므로 사과농축액에서 비효소적 갈변이 진행됨을 알 수 있었다. 하지만 갈변억제제가 첨가된 농축액에서도 비효소적 지표들의 변화가 대조구와 유사하게 나타나므로 A.A.와 PVPP 첨가에 따른 비효소적 갈변저해효과는 뚜렷하지 않다고 판단되었다. 이에 반해 PVPP 첨가 사과농축액의 경우 PPO(polyphenoloxidase)활성이 가장 낮았고 폴리페놀 함량도 저장 초기값에서 상당히 감소함을 보였다. 따라서 PVPP는 폴리페놀화 합물들을 흡착, 제거함으로써 효소적 갈변을 효과적으로 억제하는 것이 확인되었다. The study was conducted to investigate the effect of the browning inhibitors such as PVPP (polyvinylpoly- pyrrolidone), A.A. (ascorbic acid) on nonenzymatic browning factors [free sugar, total amino acid, organic acid, A.A., HMF (hydroxymethylfurfural)] and enzymatic browning factors [PPO (polyphenoloxidase) activity, polyphenol compounds] in concentrated apple juice during 90 days storage. Considering color value (L value, ΔE), absorbance at 420 nm, concentrated apple juice added with PVPP showed clearly the effect of browning inhibition. According to the storage period, the changes of nonenzymatic factors in concentrated apple juice added with browning inhibitors were similar to those in control (concentrated apple juice without browning inhibitors), which were the decrease of sucrose (0.24~0.35% at 90 days), the slight increase of glucose and fructose, the decrease of total amino acid (530.4~573.1 mg/100 g at 90 days), same value of A.A. at 90 days (38.5~78.6 mg/100 g), and the increase of HMF (27.8~30.6 mg/100 g at 90 days). On the contrary, enzymatic browning factors were significantly inhibited in concentrated apple juice added with PVPP, judging from the slow increase of PPO activity and the significant decrease of initial value in polyphenol compounds (especially chlorogenic acid). These results suggest that PVPP plays an important role as enzymatic browning inhibitor, that is, a scavenger of polyphenol compounds by adsorption in concentrated apple juice.