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Microcystis ( Cyanobacteria ) 분리주에서의 Microcystin 생산에 관한 연구
박혜경,진익렬,류홍일,류재근,도삼유평 (稻森悠平) ( Hae Kyung Park,Ing Nyol Jin,Hong Il Rhu,Jae Keun Ryu,Yuhei Inamori ) 한국물환경학회 1996 한국물환경학회지 Vol.12 No.1
Microcystis strains were isolated from water-blooms that had been collected from Korean lakes and reservoirs and cultured under uni-algal, non-axenic condition. Late exponential phase cells were harvested, concentrated and lyophilized for toxin extraction. Isolation, purification and qualitative and quantitative characterization of toxins were carried out by High Performance Liquid Chromatography(HPLC) system. Among 16 tested Microcystis strains, 14 strains produced hepatotoxic heptapeptide microcystins. The amounts and types of toxins were different according to strains. The identified toxins were microcystin-RR, -LR and YR.
한국산 유독 남조류의 독소함량을 근거로 한 조류경보제 발령기준 검토
박혜경 ( Hae Kyung Park ),김화빈 ( Hwa Bin Kim ),이재정 ( Jay J. Lee ),이재안 ( Jae An Lee ),이혜진 ( Hae Jin Lee ),박종환 ( Jong Hwan Park ),서정관 ( Jung Kwan Seo ),윤석제 ( Seok Jea Youn ),문정숙 ( Jeong Suk Moon ) 한국물환경학회 2011 한국물환경학회지 Vol.27 No.4
We investigated the ranges of total cellular microcystins content of cyanobacterial blooms collected in Korean lakes and rivers from 2005 to 2009. The amount and composition of microcystins of Korean cyanobacteria varied depending on the sampling water bodies and dominant cyanobacterial genera. Toxic cyanobacterial cell numbers equivalent to 1 μg MCYSTs/L using total cellular microcystin content of Korean cyanobacteria were in the range of 2,348 to 66,980,638 cells/mL. Only four samples among forty nine samples showed less cell numbers than current criterion of Harmful Algae Alert System, 5,000 cells/mL indicating current criterion do not reflect properly the microcystins content of Korean cyanobacteria. Anabaena and Aphanizomenon spp. showed three to six times higher cell numbers equivalent to 1 μg MCYSTs/L than Microcystis spp. To propose criteria of Harmful Algae Alert System for Korean toxic cyanobacteria, we calculated about 50% selective geometrical means of cyanobacterial cell numbers equivalent to 1 μg MCYSTs/L in order of toxic content. The proposed criteria for Microcystis, Oscillatoria, Anabaena, and Aphanizomenon spp., are 10,000, 20,000, 40,000, and 80,000 cells/mL, respectively.