가감좌귀음(加減左歸飮)의 알레르기성 기관지천식(氣管支喘息) 반응(反應) 조절(調節) 효과(效果)

박은정,이해자,박종익,박영주,Park, Eun-Jung,Lee, Hai-Ja,Park, Jong-Ik,Park, Young-Joo 대한한방소아과학회 2004 대한한방소아과학회지 Vol.18 No.2

Objective : KagamJwagwiEum(KJE) has been used for the purpose of prevention and treatment of bronchial asthma and allergic asthma in Korea. To investigate the biological effect of KJE, the author examined cytotoxicity and inflammatory cytokines secretion with human leukemic mast cell line, HMC-1. Methods: HMC-1 was stimulated with phorbol 12-myristate 13-acetate(PMA) and calcium ionophore A23187. KJE by itself had no effect on viability of HMC-l. The effects of KJE on the secretion of tumor necrosis $factor-alpha(TNF-{\alpha})$, interleukin(IL)-6 and IL-8 from HMC-1 were evaluated with enzyme-linked immunosorbent assay. Results : KJE inhibited PMA plus A23187-induced $TNF-{\alpha}$ and IL-6 secretion. But KJE had no effect IL-8 secretion: KJE had immunoregulatory effects on cytokines, increased secretion of NO and $TNF-{\alpha}$ but did not effect IL-12 secretion when the cells were primed and trigged with $IFN-{\gamma}$ in the peritoneal macrophages of C57BL/6 mice. Conclusions : Taken together, these results suggest that KJE inhibit the production of inflammatory cytokines in HMC-1 cells and activate macrophages.

소아(小兒) 천식(喘息)에 관(關)한 문헌적(文獻的) 고찰(考察)

박은정,심문경,Park Eun-Jeong,Sim Mun-Gyeong 대한한방소아과학회 1994 대한한방소아과학회지 Vol.8 No.1

생약으로부터 세포분화유도물질의 검색 및 분리 및 분리 (I)

박은정,김진웅,Park, Eun-Jung,Kim, Jin-Woong 한국생약학회 1997 생약학회지 Vol.28 No.4

300 extracts derived from 100 plants were tested for their potential to induce HL-60 cell differentiation using NBT assay and NSE/SE staining methods. Morphological changes from suspended to adherent state of the cells were also observed by microscopic examination. In result, 55 extracts induced cell differentiation into monocyte/macrophage lineage in the NBT and the NSE assay.

소아비연(小兒鼻淵)에 관한 문헌적(文獻的) 고찰(考察)

박은정,정규만,Park Eun-Jeong,Jeong Gyu-Man 대한한방소아과학회 1989 대한한방소아과학회지 Vol.3 No.1

소아비연(小兒鼻淵)에 대(對)한 임상적(臨床的) 연구(硏究)

박은정,이해자,Park Eun-Jeong,Lee Hae-Ja 대한한방소아과학회 1998 대한한방소아과학회지 Vol.12 No.1

1. In oriental medicine, the case of sinusitis can be explained, not only external invasion such as PUNG HAN(wind-cold), PUNG YUL(wind-hot), SHUP YUL(damp-heat), but also functional disorder of internal organ such as spleen(脾), lung(肺), kidney(腎) The western medicine classified the cause of sinusitis as two factors. That is bacterial infection factor-Haemophilus influenza, streptococcus pyogeues, streptococcus pneumonia- and viral infection factor-Rhinovirus, parainfluenza, Echo28, Coxsacki21, Sinusitis is complicated to allergic rhinitis, chronic otitis media purulent, chronic tonsilitis, sinubronchitis. On the condition of nasal septum deformity, turbinates deformity, nasal septum deviation, sinusitis can be developed. the predisposing factors of sinusitis is swimming, air pollution, malnutrition, shortage of immunity.2. According to survey, sinusitis occurred that children from 4 to 12 years old and from 5 to 7 years old occupied 70% 3. From the past history data, they experienced chronic tonsillar hypertropy(20%), otitis media, atopic dermatitis, allergic rhinitis, bronchial asthma, pneumonia, bronchiolitis, chronic sore throat, urticaria, milk allergy in sequence. 4. the symptoms of sinusitis is nasal obstruction, postnasal dripping, purulunt(yellow)or white discharge, cough, nose bleeding in sequence. nasal obstruction take the portion of 95%, postnasal dripping 65%, night time or early morning cough 60%. 5. The suffering period of sinusitis is 6 month minimum, 4 years maximum, most cases are included in a year. The suffering period of children was shorter than adult. 6. Diagnosis depend on inspection of nasal cavity, postnasal dripping, X-ray finding. 75% of patient(15case) showed both maxillary sinusitis, 25%(5cases) showed left or right maxillary sinusitis. 7. Treatment of oriental medicine, consist of Herb-medicine, acupuncture and exposing of Lazer beam. Kamihyunggyeyungyotang(加味荊芥蓮翹湯) is administered mainly as the medical therapy, Kamigwaghyangjeungkisan(加味藿香正氣散) Kimizwakwieum(加味左歸飮), Kamihyangsosan(加味香蘇散) is administered for a additional symptoms which occurred by influenza recurrence. Kamijeonxibackchulsan(加味錢氏白朮散) is administered to treat gastro-intestine trouble patients who have sinusitis. 8. The period of treatment is varied with patient conditions and X-ray finding. The minimal period is 35days, maximal period is 202days. So it took about 86days in average and about 50% of patient(10cases) is recovered in one or two month.

당귀음자(當歸飮子) 가감방(加減方)의 처방별 (A, B) 아토피성 알레르기반응 조절 효과 비교 연구

박은정,이해자,한경훈,Park, Eun-Jung,Lee, Hai-Ja,Han, Kyeung-Hoon 대한한방소아과학회 2005 대한한방소아과학회지 Vol.19 No.2

Objective: Danguieumja-gagambang (DGEJGB), a traditional Korean prescription, has been used as therapeutics for allergic diseases such as atopic dermatitis (AD). In this study, we compared with regulatory Effect of Atopic Allergic Reaction by Prescriptions A and by Prescriptions B. Methods : To evaluate and compare the atopic allergic effectiveness of two prescription (A, B) of DGEJGB, the author investigated a possible effect of DGEJGB on mast cell-mediated allergic reaction, cytokine secretion and mRNA expression in vivo and in vitro. Results : Mast cells are a potent source of mediators that regulate the inflammatory response in allergic reaction. In mice orally administered A, B of DGEJGB ( 0.1, 0.1 and 1.0 g/kg) for 1 h, compound 48/80-induced ear swelling was significantly reduced. Significant reduced levels (P < 0.05). of tumor necrosis factor $(TNF)-{\alpha}$ was observed in the human mast cell line (HMC-1) with DGEJGB (A). IL-6 and IL-8 secretion were significantly inhibited by DGEJGB (A, B). In addition, $TNF-{\alpha}$ and IL-8 mRNA expression were reduced by DGEJGB (A) at the dose of 0.01 mg/ml without cell toxicity. Conclusions : These results suggest that DGEJGB (A) contributes to the treatment of atopic allergic reactions rather than DGEJGB (B), and that its action may be due to inhibition of cytokine secretion and mRNA expression HMC-1.

소아중풍(小兒中風) 환자(患者)에 대한 치험(治驗) 1례(例)

박은정,이해자,나원경,장성진,안재선,한경훈,Park, Eun-Jung,Lee, Hai-Ja,Na, Won-Kyeung,Jang, Sung-Jin,Ahn, Jai-Sun,Han, Kyeung-Hoon 대한한방소아과학회 2004 대한한방소아과학회지 Vol.18 No.2

Objectives : The purpose of this study is case report of stroke in childhood. The childhood cerebrovascular diseases are interesting than those seen in adulthood, because the etiology and prognosis of the disease in children are quite different from those of adults. Cerebrovascular diseases in children is more common than once recognized. However, relatively little attention has been paid to the epidemiology of childhood stroke. Methods : We experienced one case of ischemic stroke in childhood whose symptoms are similar to that of adults. There is no evidence of cerebrovascular malformation at MRI, MRA film. The cause of stroke is undetermined and just we can see the little infarction lesion on Lt. pons. We treated the patient with herb medicine, acupuncture-Tx, negative-Tx, moxa-Tx and physical treatment. Results : After the treatment of oriental medicine, the patient recovered from the stroke symptoms. Conclusion : we thought that the more follow-up is needed. So we repoert one case of cerebral infarction in children that recovered from stroke symptoms after the treatment of oriental medicine.

당뇨병쥐 뇌에서 Gangliosides 분석

박은정,전길자,Park, Eun-Jeong,Jhon, Gil-Ja 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.6

본 연구에서는 alloxan으로 유발시킨 당뇨병 쥐의 뇌에서 gangliosides 조성 변화를 조사하였다. 뇌 조직은 alloxan 주사후 3일째 희생시켜 얻었으며, gangliosides는 Folch-Suzuki 방법을 이용하여 추출하였다. Periodate-resorcinol 법으로 sialic acids를 정량한 결과, 당뇨병 쥐 뇌에서 sialic acid 양$(375{\mu}g)$이 정상$(783{\mu}g)$에 비해 반으로 감소하였다. 당뇨병 쥐 뇌에서 GT1b, GD1b, GD1a는 감소하였고 GM3와 band 1은 증가하였다. Sialidase처리로 band 1은 sialic acid가 없는 당지질임이 확인되었다. 2D-HPTLC로 당뇨병 쥐 뇌에서 GM1a와 GD1a 사이에 존재하는 마확인된 ganglioside가 증가함이 밝혀졌다. 미지의 ganglioside를 preparative TLC로 분리하여 ${\beta}-galactosidase$를 처리한 후 HPLC로 확인한 결과 Gal-GM1, Gal-Gal-GM1임이 확인되었다. The changes of ganglioside pattern from alloxan-induced diabetic rat brain, were investigated. Gangliosides were isolated by Folch-Suzuki method and analyzed by HPTLC. Sialic acid was determined by periodate-resorcinol method. The amount of gangliosides decreased 2 folds in diabetic brain. We found decreasing GT1b, GD1b, and GD1a, increasing GM3 and band 1, and no change of GM1a. In diabetic brain band 1 was identified as simple glycolipid with no sialic acid by the treatment of sialidase. The increase of unidentified ganglioside separated between GM1a and GD1a in diabetic brain found by 2-dimensional HPTLC. Unidentified ganglioside was collected by preparative HPTLC, treated with ${\beta}-galactosidase$ and analyzed by HPLC. As the result, this ganglioside revealed Gal-GM1 and Gal-Gal-GM1.

Effects of HyangsosanGamibang on the $PPAR{\gamma}$ in the bronchial asthma mouse model

박은정,이해자,오지은,Park, Eun-Jung,Lee, Hai-Ja,Oh, Ji-Eun The Association of Korean Oriental Pediatrics 2007 대한한방소아과학회지 Vol.21 No.1

목적 : OVA에 유도된 천식 쥐 모델에 향소산가미방을 투여한 후 $PPAR{\gamma}$의 변화를 조사하여 향소산가미방의 천식 치료 기전을 알아보고자 하였다. 대상 및 방법 : 8주된 암컷 BALB/c 마우스에 첫 날과 14일 후에 20 ${\mu}g$의 OVA를 알루미니윰 하이드록사이드 1mg과 혼합한 후 총 200 ${\mu}g$를 복강 내로 주입하여 감작시켰다. 처음 감작시킨 날로부터 21,22,23일 후에 천식 모델에 사용하는 초음파 분무기를 이용하여 세 번째 감작시켜서 천식 마우스 모델을 만들었다. 천식 마우스 모델을 만드는 기간 중 OVA를 복강 내로 주입한 후 19일째에 24시간의 간격으로 향소산가미방을 7일 동안 경구 투여하여 향소산가미방의 효과를 조사하였다. 기관지폐포세척술은 마지막 감작 후 72시간 후에 실행하고 기관지폐포 세척액의 총 세포수를 측정하였다. $PPAR{\gamma}$의 발현은 천식 마우스 모델의 폐와 향소산가미방을 투여한 마우스 모델의 폐를 적출한 후 Western blotting 방법을 아용하여 측정하였다. 병리 조직학적 검사는 hematoxylin 2 and eosin-Y 염색을 이용하여 조사하였다. 결과 : 정상 군과 비교하여 OVA감작 천식 쥐 모델에서는 72시간 후에 총 세포 수가 증가하였다. 특히 OVA감작 천식 군에서 증가된 호산구의 수가 향소산가미방을 투여 한 쥐 군에서는 유의하게 감소하였다. OVA감작 천식 쥐 모델에서 72시간 후에 정상 군과 비교하여 핵 내에서의 $PPAR{\gamma}$단백질의 발현이 약간 증가하였다. 그러나 향소산가미방을 투여한 쥐 모델에서는 세포질과 핵 내에서 $PPAR{\gamma}$단백질의 발현이 유의하게 증가하였다. 조직학적 검사상 정상 군과 비교하여 OVA 감작된 천식 쥐 모델에서는 폐포, 세기관지, 기도내강 주변에 많은 염증 세포들이 있었다. 그러나 가미향소산 을 투여 한 후에는 염증 세포들이 유의하게 감소하였다. 결론 : 가미향소산은 $PPAR{\gamma}$작용제로서 역할을 하며, 천식에 대한 치료제 또는 예방제를 개발하는 데 후보 물질이 될 것으로 사료된다.

유비쿼터스 멀티미디어 응용을 위한 그리드 시스템의 서비스 플랫폼

박은정,신현식,Park Eun-jeong,Shin Heon-shik 한국통신학회 2006 韓國通信學會論文誌 Vol.31 No.1B

무선 통신의 발전으로 인하여 PDA, 휴대전화 등의 이동기기를 위한 멀티미디어 서비스의 개발 또한 빠르게 발전하고 있다. 이러한 모바일 멀티미디어 서비스의 개발에는 모바일 컴퓨팅의 요구조건을 효율적으로 제어할 수 있는 플랫폼이 필요하다. 본 논문에서는 무선 통신의 잦은 통신단절과 모바일 디바이스의 시스템 자원부족에 대응하기 위하여 모바일 에이전트 기반의 그리드 시스템을 사용하는 적응형 서비스 플랫폼을 제시하고, 서비스 플랫폼의 활용예로 모바일 원격 교육 또는 화상 회의에 사용되는 RtoA (Ready-to-Attend) 프레임워크를 구성하였다. RtoA는 사용자들에게 원활한 유비쿼터스 서비스를 제공하기 위하여 그리드 시스템을 이용한 핸드오프, 스피커, 리스너 서비스를 제공한다. ns-2를 이용한 성능평가의 결과 RtoA는 모바일 디바이스의 에너지 소모량을 줄이고, 응답시간을 향상시키는데 효율적임이 증명되었다. Advances in wireless network are enabling the development of ubiquitous multimedia services. These multimedia services need efficient platforms to comply with the requirements of mobile computing. We introduce an adaptive service platform based on mobile agent and grid systems while specifying the challenges of ubiquitous multimedia services and focusing on frequent disconnections and scarce resources. We applied our platform to framework RtoA (Ready-to-Attend) which supports mobile users to access compute-intensive multimedia service, specifically, mobile education and video conferencing. RtoA includes hand-off, speaker and listener service which enable people to attend a conference or a class with satisfying quality of multimedia service. ns-2 based simulation verifies that our scheme is an efficient way to reduce energy consumption of mobile devices and to improve the response time of mobile applications.