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디엠프리(녹차추출물)에 의한 나균 감염 중간엽줄기세포의 IL-6 생산 억제
박란숙,Park, Ran-Sook 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.4
Previous reports revealed that DMfree (green tea extract) inhibited expression of the IL-6 gene in Mycobacterium lepraeinfected MSCs (mesenchymal stem cells). This study aimed to measure IL-6, $IL-1{\beta}$, $TNF-{\alpha}$ and PGE2 production in M. leprae-infected MSCs using ELISA. To confirm the effect of DMfree on IL-6 and signal transduction, a western blotting test was performed. DMfree inhibited the expression of IL-6 in the MSCs and the heterodimer of STAT3, which also affects the expression of multiple genes. Though DMfree pre-treatment of control MSCs produced a baseline level of IL-6, it significantly inhibited the production of IL-6 in M. leprae-infected MSCs. There was no significant difference in IL-6 production between 1 and 7 day treatment groups. M. leprae-infected MSCs produced more $IL-1{\beta}$, $TNF-{\alpha}$ and PGE2, but DMfree could not inhibit their production at a physiological concentration. This is different from other reports that used higher concentration of EGCG treatment, resulting in significant inhibition of the cytokines. The inhibition appears to be related to the concentration of EGCG. These results indicate that DMfree can alleviate inflammation involving IL-6.
디엠프리(녹차 추출물)가 나균 감염 중간엽 줄기세포의 유전자 발현에 미치는 영향
박란숙,Park, Ran-Sook 한국식품영양학회 2014 韓國食品營養學會誌 Vol.27 No.2
This study found antibacterial activity of $DMfree^{(R)}$ [green tea extract] on facultative bacteria by direct petri dish method and gene array of obligatory M. leprae infected mesenchymal stem cells (MSC). While DMfree showed DPPH radical scavenging effect and high contents of polyphenol, it did not inhibit growth of facultative bacteria such as E. coli and S. aureus on the petri dish. The result does not exclude a possible antibacterial effect of organic solvent extract of green tea rather than DMfree which comes from the water extract of green tea. Pre-treatment of DMfree appeared to have no effect on copy number of 14 genes compared with control MSC by real-time RT-PCR. However pre-treatment of DMfree on M. leprae infected MSC revealed a significant decrease of anti-inflammatory cytokine (IL-6), (P<0.038) and sharp down-regulation of pro-inflammatory cytokine (IL-1). Enhanced expression of VEGFR-1 mRNA was noted in DMfree pretreated M. leprae infected MSC group (P<0.003). These results show that DMfree would stabilize M. leprae infected MSC from further inflammation by down-regulating anti-inflammatory cytokine (IL-6) and pro-inflammatory cytokine (IL-$1{\beta}$). This is the first report on DMfree inhibition of IL-6 and IL-$1{\beta}$ expression in M. leprae infected MSC. Further experiments that detect protein levels of IL-$1{\beta}$ and IL-6 may support the result of this gene array.
마늘성분 SAC 및 Hydrogen Peroxide에 의한 줄기세포의 유전자 발현 윤곽
박란숙,Park, Ran-Sook 한국식품영양학회 2012 韓國食品營養學會誌 Vol.25 No.4
Though hydrogen peroxide ($H_2O_2$) causes a deleterious effect to cells with its reactive oxygen species resulting in cell death, S-allyl cysteine (SAC, a bioactive organosulfur compound of aged garlic extract) has been known to have a cytoprotective effect. Few reported profiles of gene expression of $H_2O_2$ and SAC treated human cord blood derived mesenchymal stem cells (MSC). This study revealed changes in the profile of twenty-one genes grouped by oxidative stress, antioxidant, cell death, anti-apoptosis and anti-aging by quantitative real time PCR. A concentration of $100{\mu}M$ of SAC or $50{\mu}M$ of $H_2O_2$ was applied to MSC which show moderate growth and apoptosis pattern. $H_2O_2$ treatment enhanced expression of eleven genes out of twenty-one genes compared with that of control group, on the contrary SAC suppressed expression of eighteen genes out of twenty-one genes except C ros oncogene. SAC decreased expression of oxidative stress genes such as SOD1, CAT and GPX. These results seemed consistent with reports which elucidated over-expression of NF-${\kappa}$B by $H_2O_2$, and suppression of it by SAC. This study will confer basic information for further experiments regarding the effects of SAC on gene levels.
산소 농도의 변화와 물리적 손상이 제대혈 중간엽 줄기세포의 증식에 미치는 영향
박란숙 ( Ran Sook Park ) 한국식품영양학회 2011 韓國食品營養學會誌 Vol.24 No.4
Human umbilical Mesenchymal Stem Cell(uMSC) has been known as one of major component to regenerate connective tissues such as bone, cartilage, fat and others. The effect of low(5%), normotensive(20%) oxygen and freezing-thawing damage on proliferation of uMSC were investigated. low oxygen concentration culture of uMSC resulted in enhanced proliferation significantly(p<0.05) than 20% of oxygen culture. After the freezing-thawing injury to uMSC, 5% oxygen culture showed marked proliferation of uMSC than that of 20% oxygen(p<0.05) in the 5th passage of uMSC. Expression of antioxidant enzymes such as superoxide anion 1 and glutathione peroxidase 1 appeared marked in 20% oxygen cultured uMSC, which suggest oxidative stress could induce less proliferation of uMSC. Above findings would suggest prolferation of uMSC in 5% of oxygen will give more yields.
서울 일부 지역 비만 여대생의 체성분, 활동량, 식이 섭취량 분석
박란숙 ( Ran-sook Park ) 한국고등직업교육학회 2009 한국고등직업교육학회논문집 Vol.10 No.2
The purpose of this study was to determine body composition parameters, dietary intakes, and physical activities in female college students. The subjects were divided into three groups, standard, obese, heavy obese, according to fat mass(%) with tool of bioelectrical impedence (Inbody 3.0Ⓡ). We adopted Can-ProⓇ program to assay their dietary intakes for weekdays and weekends to obesity and physical activities. The fat mass(%) of each group was significantly associated with body mass index(BMI) and WHR(waist-hip ratio). There was significant difference among the groups regarding to intakes of dietary fiber(p<0.05) and sodium(p<0.05) between weekdays and weekends. The subjects in this study consumed only 69% of KDRIS(Dietary References Intakes for Korean) for energy intakes(1400 Kcal), 57% for dietary fiber, 53% for calcium, and 58% for iron, respectively. Heavy obese-group had significant lower intakes of dietary fiber(p<0.05), calcium(p<0.05), and iron(p<0.05) comparing with control group. They were revealed to have higher calorie-intakes from carbohydrates and lower calorie-intakes from breakfast. In conclusion, about 33% of female collage students had extremely high body fat-mass, unbalanced dietary intakes, and lack of physical activity, including long TV viewing hours per day. This study suggests that nutrition education is needed for female college students to improve unbalanced eating habits and inappropriate body weight.