Horseradish peroxidase가 변성된 탄소복합 바이오센서 개발 및 전기화학적 H₂O₂분석

박덕수,Park, Deog-Su 대한화학회 2012 대한화학회지 Vol.56 No.5

Horseradish peroxidase (HRP), alkoxysilane인 TEOS의 겔 용액과 graphite를 혼합하여, 전기전도성을 나타내고 재사용이 가능하며 안정성을 향상시킨 carbon composite electrodes (CCEs) 바이오센서를 제작하였다. TEOS의 졸-겔 전이반응을 이용하여 HRP를 silicate gel network포획시킨 바이오센서는 -0.2 V에서 $H_2O_2$의 환원이 시작되었으며, HRP의 함량과 pH를 변화시켜 최적 분석 조건을 구하였다. 분석 최적 조건에서 검량선 및 검출한계를 구한 결과, 0.2~2.2 mM의 $H_2O_2$ 농도 범위에서 직선적인 감응을 나타내며 검출한계는 0.035 mM이었다. ascorbic acid, acetaminophene, uric acid의 방해작용을 조사한 결과 $H_2O_2$의 전기화학적 분석에 영향을 주지 않았다. A sol-gel derived carbon composite electrodes (CCEs) were fabricated by mixing horseradish peroxidase (HRP), sol of tetraethoxysilane (TESO), and graphite powder. The HRP solution was added to the sol solution of TEOS, and then graphite powder was added to this mixture. The resulting carbon ceramic network effectively encapsulated HRP and shows a catalytic reduction starting at -0.2 V for $H_2O_2$. The optimum conditions for $H_2O_2$determination have been characterized with respect to the enzyme loading ratio and pH. The linear range and detection limit of $H_2O_2$ detection were from 0.2 mM to 2.2 mM and 0.035 mM, respectively. The common electroactive interferences such as ascorbic acid, acetaminophene, and uric acid were not affected upon the response to $H_2O_2$ at the HRP biosensor due to low detection potential.

Laser ablation을 이용한 폴리이미드 필름 전극제조 및 전기화학적 글루코오즈 바이오센서 응용

박덕수 ( Deog Su Park ) 한국센서학회 2013 센서학회지 Vol.22 No.5

An ultraviolet pulsed laser ablation of polyimide film coated with platinum has been used to enhance the sensitivity for the application as an electrochemical biosensor. Densely packed cones are formed on polyimide surface after UV irradiation which results in increase of surface area. In order to apply the sensitivity improvement of laser ablated polyimide film electrodes, the glucose oxidase modified biosensor was fabricated by using an encapsulation in the gel matrix through sol-gel transition of tetraethoxysliane on the surface of laser ablated polyimide film. The optimum conditions for glucose determination have been characterized with respect to the applied potential and pH. The linear range and detection limit of glucose detection were from 2.0 mM to 18.0 mM and 0.18 mM, respectively. The sensitivity of glucose biosensors fabricated with laser ablated polyimide film is about three times higher than that of plain polyimide film due to increase in surface area by laser ablation.

파라세타몰 검출을 위한 전기화학적 다중효소 바이오센서

박덕수 ( Deog Su Park ),심윤보 ( Yoon Bo Shim ),장승철 ( Seung Cheol Chang ) 한국센서학회 2008 센서학회지 Vol.17 No.1

탄소섬유전극을 이용한 흐름형 전기분해조 제작 및 유기염료의 전기화학적 분해 연구

박덕수 ( Deog Su Park ) 한국환경과학회 2012 한국환경과학회지 Vol.21 No.11

The simulated dyes solution containing Basic Red 46(BR 46), Yellow 21(Y 21), and Maxilon Blue 30(MB 30) were electrochemically oxidized using carbon fiber as an anode. The electrolyses were performed in a electrolytic flow cell constructed by Vycor glass tube. The carbon fiber was positioned in the inside of Vycor glass tube and platinum wire coiled around outside of tube as a cathode. Several operating variables, such as current, time, pH and flow rate of solution were studied. Increasing current density would lead to a corresponding increase in the dye removal efficiency 99.2 % at a 200 mA. The electrolyses time could also improve and removal efficiency was about 99 % after 1.5 hours of electrolyses. The removal efficiency was increased with the increase of flow rate of solution and optimum flow rate was 5 mL/min. THe pHs of solution affect the removal efficiency. The removal efficiency was decreased with the increase of pH of solution and optimum pH was 5.05 (0.1 M KNO3).

휴믹산을 이용한 중금속 제거효율에 대한 연구

박덕수(Deog Su Park),심윤보(Yoon Bo Shim),정의덕(Euh Duck Jeong),원미숙(Mi Sook Won) 大韓環境工學會 1999 대한환경공학회지 Vol.21 No.4

${\iota}$ -Sparteine으로 변성된 Carbon Paste 전극을 사용한 Co(II) 이온의 펄스 차이 전압-전류법 정량

정의덕,원미숙,박덕수,심윤보,최성낙,Eu-Duck Jeong,Mi-Sook Won,Deog-Su Park,Yoon-Bo Shim,Sung-Nak Choi 대한화학회 1993 대한화학회지 Vol.37 No.10

코발트(II) 이온의 정량을 위해 ${\iota}$-sparteine(SP)으로 변성된 탄소 반죽 전극(carbon paste electrode : CPE)을 제작하였다. 수용액 중의 코발트 이온을 CPE에 처리시킨${\iota}$-sparteine과 반응시켜서 착화합물 상태로 전극표면에 석출시킨 후, 이 착물을 전압-전류법을 사용하여 정량하였다. 또한 변성된 CPE의 전극반응을 순환 전압-전류법을 사용하여 아세트산/아세트산염 완충용액에서 조사하였다. 한번 사용한 전극은 산용액으로 처리하여 5회 이상 재사용할 수 있었다. CPE에 흡착된 Co(II)-SP 착물의 산화환원 전위는 0.17V / 0.27V였으며 산화피이크는 0.17V의 환원피이크를 지나야 나타나는 피크이다. 이는 전극표면의 SP와 용액내의 Co(II)이온이 착물을 형성하여 흡착되고, 이 전극표면에 형성된 착물이 산화환원되어 나타나는 피이크이다. 펄스차이 전압-전류법을 사용할 경우 $2{\times}10^{-7}$M 농도까지 직선적으로 감응함을 알 수 있었으며, 그 때의 상대표준 편차는 ${\pm}$5.6%이었다. 이 경우 검출한계는 $1.0{\times}10^{-7}$M이었다. 리간드와 착물을 형성할 것으로 예상되는 여러 금속이온에 대한 방해작용을 조사하였다. A cobalt(II) ion-selective carbon-paste electrode (CPE) was constructed with ${\iota}$-sparteine. Cobalt(II) ion in aqueous solution was chemically deposited through the complexation with ${\iota}$-sparteine onto the CPE. The surface of CPEs were characterized by cyclic voltammetry and differential pulse voltammetry in an acetate buffer solution, separately. Exposure of the CPEs to an acid solution could regenerate surface to reuse it for the deposition. In more than 5 deposition / measurement / regeneration cycles, the response was reproducible and linear up to $5.0{\times}10^{-6}$M with linear sweep voltammetry. The peaks at 0.17V / 0.27V were correspond to the redox of Co(II)-SP complex deposited on CPE. The anodic peak of which appeared after scan over the cathodic peak of 0.17 V to more negative scan. In case of using the differencial pulse voltammetry (DPV), we have obtained the linear response $2.0{\times}10^{-7}$M with relative standard deviation ${\pm}5.6%$. The detection limit was $1.0{times}10^{-7}$M for 20 minutes of the deposition. We have also investigated the interference effect of various metal ions, which are expected to form the complex with the ligand on the electrode.

Tetraethoxysilane의 졸-겔 반응을 이용한 전기화학적 glucose biosenor 개발

장승철 ( Seong Cheol Chang ),박덕수 ( Deog Su Park ) 한국센서학회 2012 센서학회지 Vol.21 No.4

Disposable amperometric screen-printed biosensor strips have been fabricated by a sol-gel encapsulation for the analysis of glucose. The glucose oxidase(GOx) is entrapped in the gel matrix through sol-gel transition of tetraethoxysliane(TEOS). The biosensor is fabricated by GOx containing thin film of TEOS gel on the surface of screen-printed carbon electrode(SPCE). The GOx-containing thin film of TEOS gel offers a one-step modification process on the surface of SPCE. The optimum conditions for glucose determination have been characterized with respect to the applied potential, enzyme loading ratio, and pH. The linear range and detection limit of glucose detection were from 2.0 mM to 16.0 mM and 0.25 mM, respectively.

Disposable in-field electrochemical potable sensor system for free available chlorine (FAC) detection

장승철 ( Seung Cheol Chang ),박덕수 ( Deog Su Park ) 한국센서학회 2007 센서학회지 Vol.16 No.6

졸-겔 방법에 의한 LiMn₂O₄의 합성 및 전기화학적 거동

정의덕,문성욱,이학명,원미숙,윤장희,박덕수,심윤보,Jeong, Euh-Duck,Moon, Sung-Wook,Lee, Hak-Myoung,Won, Mi-Sook,Yoon, Jang-Hee,Park, Deog-Su,Shim, Yoon-Bo 한국전기화학회 2003 한국전기화학회지 Vol.6 No.4

리튬이온전지 양극재료로서 리튬막간계 산화물을 졸겔 방법으로 전구물질을 합성하여 $400^{\circ}C$와 $800^{\circ}C$에서 열처리하여 합성하였다. 출발물질로는 $(CH_3)_2CHOLi$와 $Mn(CH_3COO)_2\;{\cdot}4H_2O$를 사용하였다 열분석 측정을 통해 열처리 조건을 정하였다. 또한 합성된 물질은 X-선 회절분석으로 구조를 확인하였으며 형상 및 입자의 크기는 주사전자현미경으로 측정하였다. 전기화학적 특성은 1.0M $LiClO_4/propylene\;carbonate(PC)$ 전해액 조건에서 순환전압전류법, chronoamperometry, 교류 임피던스 법 및 정전류 충$\cdot$방전 특성을 조사하였다. 교류 임피던스 법으로 확산계수를 측정한 결과 $6.2\times10^{-10}cm^2s^{-1}$를 나타내었다. A precursor of lithium manganese oxide was synthesized by mixing $(CH_3)_2CHOLi\;with\;Mn(CH_3COO)_2{\cdot}4H_2O$ in ethanol using a sol-gel method, then heat-treated at $400^{\circ}C\;and\;800^{\circ}C$ in air atmosphere. The condition of heat treatment was determined by thermogravimetric analysis/differential thermogravimetric analysis (TGA/DTA). The characterization of the lithium manganese oxide was done by X-ray diffraction (XRD) spectra and scanning electron microscopy (SEM). The electrochemical characteristics of lithium manganese oxide electrode for lithium ion battery were measured by cyclic voltammetry (CV), chronoamperometry and AC impedance method using constant charge/discharge process. The electrochemical behaviors of the electrode have been investigated in a 1.0M $LiClO_4/propylene$ carbonate electrolyte solution. The diffusivity of lithium ions, $D^+\;_{Li}\;^+$, as determined by AC impedance technique was $6.2\times10^{-10}cm^2s^{-1}$.

유기산을 이용한 납 요염토양의 복원에 관한 연구

정의덕,강신원,박덕수,신학기,백우현 한국환경과학회 2000 한국환경과학회지 Vol.9 No.5

A study on the removal of Pb ion from Pb-contaminated soil was carried out, using ex-situ extraction process. Tartaric acid (TA) and iminodiacetic acid sodium salt (IDA) as a washing agent were evaluated as a function of concentration, reaction time, mixing ratio of washing agent and recycling of washing agent. TA showed a better extraction performance than IDA. The optimum washing condition of TA and IDA were in the ratio of 1 : 15 and 1 : 20 between soil and acid solution during 1 hr reaction. The total concentrations of Pb ion by TA and IDA at three repeated extraction, were 368.8 ppm and 267.5 ppm, respectively. The recovery of Pb ion from washing solution was achieved by adding calcium hydroxide and sodium sulfide, form the precipitation of lead hydroxide and lead sulfide, and optimum amounts of sodium sulfide and calcium hydroxide were 7 g/ℓ for the TA washing solution and 4 g/ℓ , 5 g/ℓ for the IDA washing solution, respectively. The efficiency of recycle for TA and IDA washing solution were 78.8%, 95.1% and 89.2%, 96.6%, at third extractions under Na_2S and Ca(OH)_2, respectively.