닭 티푸스의 발생상황과 Salmonella gallinarum의 항균제 감수성

박노찬 ( No Chan Park ),도재철 ( Jae Cheul Do ),조광현 ( Kwang Hyun Cho ),장성준 ( Sung Jun Chang ),권헌일 ( Heon Il Kwon ),박덕상 ( Duk Sang Park ) 한국가축위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.2

The present study was conducted to investigate the prevalent characteristics of Fowl Typhoid and antibiotic susceptibility of Salmonella gallinarum isolated from 56 infective or dead chickens of 20 egg laying farms in Kyung Buk province during the period from August to December 1994. 1. Among 416,000 chickens of 92 flocks in 20 egg laying farms, 17,360 chickens of 31 flocks were died of Fowl Typhoid. 2. Salmonella gallinarum was isolated from 56 chickens in liver and spleen, and then blood of infective chickens was positive to Pullorum antigen. 3. In the survey of gross lesion of 56 chickens, 43 chickens(76.8%) were swelled at liver, 39(69.6%) were swelled at spleen, 12(21.4%) were changed with bronze, 3(5.4%) were hemorrhagic in peritoneal cavity. 4. In transmission pattern, 4 farms were outbreaked the entrance of chicken house at first, but the others were outbreaked at various place. They were transmitted at right and left directions in flock. 5. 2 farms confirmed at the early stage of infection were eradicated by removing infective chickens and administrating antibiotics, but 18 farms at chronic stage were not. 6. The biochemical properties of 112 Salmonella gallinarum from chickens were generally identical to those of the referance, but H2S was not productive, cellobiose was fermentive. 7. Minimum inhibitory concentration(MIC) of 20 isolates was performed by using. 21 antibiotics, MICs of Amikacin(Ak), Gentamicin(Gm), Kanamycin(Km), and Tetracycline(Tc) were below 1.6 ug/ml, Ampicillin(Am), Furazolidone(Fu) and Neomycin(Nm) were below 3.1 ug/ml, Cephalothin(Ce), Cefazoline(Cf) and Chloramphenicol(Cm) were below 6.3 ug/ml, Nalidixic acid(Na), Polymyxin(Po) and Rifampicin(Rf) were below 12.5 ug/ml, Penicillin (Pm) was below 25 ug/ml, Colistin(Co) and Streptomycin(Sm) were below 50 ug/ml, Sulfamerazine(Sr) and Sulfamethazine(St) were below 200 ug/ml, Lincomycin(Lm) and Spiramycin(Sp) were below 400 ug/ml, Bacitracin(Ba) was below 800 ug/ml. 8. Among the 20 isolates, all(100%) of those were sensitive to Ak, Am, Ce, Cf, Cm, Fu, Gm, Km, Na, Nm, Po, Rf, Sr, St and Tc, but 6 isolates(30%) were resistent to Co, 20(100%) to Ba, Lm, Pm, Sm, and Sp. The drug resistance patterns were simple which 6 strains were BaCoLmPmSmSp type, and 14 were BaLmPmSmSp type.

소의 제병환(蹄病患)에 관한 연구(硏究)

오강희 ( Kang Hee Oh ),박노찬 ( No Chan Park ),김이준 ( Lee Zun Kim ),박덕상 ( Duk Sang Park ) 한국가축위생학회 1990 韓國家畜衛生學會誌 Vol.13 No.2

In order to know the incidence of foot rot in herds and slaughter houses, a total of 3,665 cattles were examined the incidence rate, vaccination, and drug susceptibility the during the period from June to Novemer 1989. Eighty nine(2.42%) of 3,665 cattles were affected by the foot rot, and the highest incidence was observed on July and August. The foot rot was affected more frequently in hind limbs than frort limbs. In the vaccinated cattles of non affected herds, the disease was not occurred after-wards. Affected cattles which were treated with saturated picric acid gave more effective than those with 10% cupuric acid or 5% formalin in comarative treatment test.

영남지방 도축돈에 대한 폐렴발생 조사

조광현 ( Kwang Hyun Cho ),박인화 ( In Hwa Park ),도재철 ( Jae Cheul Do ),장성준 ( Sung Jun Chang ),박노찬 ( No Chan Park ),권헌일 ( Huon Il Kwon ),박덕상 ( Duk Sang Park ) 한국가축위생학회 1996 韓國家畜衛生學會誌 Vol.19 No.2

Lungs from 109 slaughter pigs with gross lesions indicating enzootic Pneumonia of pigs(EPP) and 16 grossly normal lungs, all originating from seven different herds, were subjected to microbiological examinations. The microbiological studies were included both bacterial and mycoplasmal culture. From lungs of 125 slaughter pigs, 87.2% pigs were pneumonia lesions alone or complexly. Mycoplasma spp., pasteurella multocida (P. multocida), Streptococcus spp., and Actinobacillus pleuropneumoniae(A. pleuropneumoniae) were detected in 39.4%, 42.2%, 13.8%, and 3.7% of the pneumonic lungs, respectively. P. multocida was the most frequently isolated organism in pneumonic lungs. Mycoplasmas not isolated organism in 33.9% the pneumonic lungs even if there are gross lessions mycoplasmas. The amounts of pneumonia in lungs with Mycoplasma spp. alone, a concurrence of Mvcoplasma spp. and P. multocida, P. multocida alone, a concurrence of P. multocida and A. pleuropneumoniae, and a concurrence of Mycoplasma spp. and A. pleuropneumoniae were 10.1%, 22.7%, 18.7%, 25%, and 30%, respectively. These findings indicated that P. multocida might be involved in the pathogenesis of pneumonia in slaughter pigs. Mycoplasma spp. was also, in this study, associated with higher frequency of pneumonia. The frequency of pigs snout lesion grade 0~5 inclusive were 27.2%, 28%, 19.2%, 16%, 6.4%, and 3.2% from 125 slaughter pigs. 32(25.6%) pigs were positive and 13~30% in the pigs from seven herds were found to be infected with atrophic rhintis(AR). A total of 46 P. multocida strains in pneumonic lungs were further characterized by capsular serotyping and testing for production of dermonecrotic toxin. 42(91.3%) of strains were capsular A and 4(8.7%) were type D. Out of the type A and type D strains, 86% and 75% were toxigenic, respectively.

설사자돈(泄瀉子豚) 유래(由來) 대장균(大腸菌)의 항생물질(抗生物質) 내성(耐性)에 관(關)하여

조광현 ( Kwang Hyon Cho ),박노찬 ( No Chan Park ),권헌일 ( Hun Ill Keun ),김이준 ( Lee Zun Kim ),박덕상 ( Duk Sang Park ) 한국가축위생학회 1992 韓國家畜衛生學會誌 Vol.15 No.2

The present study was conducted to investigate the biochemical characteristics and antibiotic resistance of pathogenic Escherichia coli(E. coli) isolated from piglets with diarrhea in Kyongbuk province during the period from February to November 1991. 368 E. coli strains were isolated from 382 piglets with diarrhea and the biochemical and cultural reaction were compared with the classification criteria of Edwards and Ewing. Tetracycline and sulfadimethoxine were found to be highly ineffective at in vitro inhibition of the E. coli of piglets origin. The majority of E. coli were susceptible to amikacin, chloramphenicol and gentamicine. 89(89.0%) of 100 strains of E. coil were resistant to one or more drugs. The organisms resistant to 20 or 3 drugs were 54(60.6%) of 89 strains, whereas 16(17.9%) strains were found to be resistant to one drug. 55(61.8%) out of 89 drug resistance strains carried R factors(R+) which were transferable to the recipients by conjugation.

호흡기질병 감염 송아지에서 분리한 Pasteurella haemolytica의 생화학적 특성 및 약제 감수성

조광현 ( Kwang Hyon Cho ),박노찬 ( No Chan Park ),권헌일 ( Hun Ill Keun ),김이준 ( Lee Zun Kim ),박덕상 ( Duk Sang Park ) 한국가축위생학회 1991 韓國家畜衛生學會誌 Vol.14 No.2

The present study was conducted to investigate biochemical properties and antimicrobial drug susceptibilities of 36 strains of Pasleurella haemolytica(P haemolylica) isolated from pneumonic calves in Kyongbuk province during the period from January 1990 to December 1990. P haemolytica was isolated from 36 of 111(32.4%) pneumonic calves of 1 to 6 months of age. The majority of biochemical and cultural properties of P haemolylica isolated from calves were identical to those of the reference strains employed. All isolated were susceptible to baytril, gentamicin, and kanamycin, some of them were resistant to tetracycline, amikacin, streptomycin, and ampicillin.

돈 폐염 유래 Pasteurella Multocida 혈청형 및 약제 감수성

오강희 ( Kang Hee Oh ),박노찬 ( No Chan Park ),김이준 ( Lee Zun Kim ),박덕상 ( Duk Sang Park ) 한국가축위생학회 1990 韓國家畜衛生學會誌 Vol.13 No.1

The present study was conducted to investigate the incidence of pasteurella multocida infection in kyungbuk swine herds during the period from July 1989 to November 1989 and some properties of the isolated organisms. P. multocida was isolated from lungs of 155 slaughtered pigs, 43(27.7%) pigs were culture positive. The majority of biochemical and cultural properties of the P. multocida isolates were identical to those of the standard strains. The capsular serogroups and drug susceptibility of 43 isolates of P. multocida from pigs with pneum-onic lesions were investigated. P. multocida isolates were typed for capsular serogroupes A by hyaluronidase inhibition of capsule and D by acriflavin auto agglutination. Most isolates(60.4%) were type A, 18.6% were type D, and the remaining 21.0% were untypable. In antimicrobial susceptibility test these isolates of P. multocida were susceptible in order of ampicillin (86.0%), trimethoprim sulfamethoxazole(83.7%), colistin (81.4%), chloramphenicol (79.1%), but the majority of them were resistant in order of streptomycin( 30.2%), triple sulfa (4.6%).

경북지방 소 Akabane병 발생과 중화항체가 분포조사

오강희 ( Kang Hee Oh ),박로찬 ( No Chan Park ),권헌일 ( Heun Il Gwon ),김이준 ( Lee Zun Kim ),박덕상 ( Duk Sang Park ) 한국가축위생학회 1991 韓國家畜衛生學會誌 Vol.14 No.1

A considerably high rate of abnormal deliveries of unknown etiology was observed among dairy cattles from November 1988 to February 1989 and Korean native cattles from January to April 1990. The abnormal deliveries consisted of abortions, stillbirths and calf deformities refers to as congenital arthrogryposis hydranencephaly (AH)syndrome. In order to know the level of Akabane antibody of dairy cattle raised in Kyungbuk province, serum neutralization test was conducted with Akabane virus(OBE-1 strain) and HmLu(Hamster lung) cell line. The results were summarized as follows. 1. During 4 months(Nov. 1988-Feb. 1989), abortion (3 heads), stillbirth(1 head) and congenital abnormalities(13 heads) of newborn were occurred in 17 dairy cattles raised in Kyungbuk province. 2. During 4 months(Jan. Apr. 1990), stillbirth(2 heads) and congenital deformities (13 heads) of newborn were occurred in 15 Korean native cattles raised in Kyungbuk province. 3. In Fey, and Apr. 1990, 1,005 dairy cattles at 99 farms were investigated on the actual condition of possessing Akabane antibody. The result was that 1,000 heads (99.9%) in 1,005 dairy cattles reacted as positive condition in Akabane antibody. The antibody titer was from 4 to over 256. 4. 189 heads (18.8%) of 1,005 dairy cattles werw below antibody titer 8 and 816 heads (81.2%) were over 16. 5. Akabane antibody titer of east coast legions(Pohang Yeongil etc) was all over 16, that of internal legions (Yeongju, Andong. etc) was relatively low. The rusult suggest that the vaccination for Akabane disease will be unnecessary for the time being because of possessing higher antibody titer reaction except the newly introduced cattle and Akabane virus was widely disseminated in kyungbuk province during the summer months in 1987 or 1988.

칼륨 이온선택성 막전극

박덕상,윤영자 崇實大學校 1999 論文集 Vol.29 No.1

The membrane electrode was prepared using Dibenzo-18-crown-6 as ionophore, DOS(dioctyl sebacate) as plasticizer, lipophilic additive (KTpCIPB) in a PVC( poly(vinyl chloride) ) matrix. The potential response of alkali, alkali earth and transition metal cations were investigated by using Dibenzo-18-crown-6 membrane electrode as working electrode. Dibenzo-18-crown-6 membrane electrode exhibits a near-Nernstian response to K+ in a concentration range from 1.0 × 10-6 M ∼ 1.0 × 10-lM with the slope of 54.1 mV/decade.

Formocresol이 齒髓組織에 미치는 影響에 關한 實驗的 硏究

朴德相 大韓齒科保存學會 1980 Restorative Dentistry & Endodontics Vol.6 No.1

AbstractThe purpose of this study was to evaluate the pulpal response against calcium hydroxide and zinc oxide containing various concentration of formocresol (0.1, 0.5, 1, 5, 10, 20 and 30%). The experiment was performed on dog's teeth (75 teeth from 5 dogs: Table 1.) and the teeth were routinly treated in laboratory procedures.Followings are the results obtained through microscopical examination. 1. In zinc oxide group, inflammatory reaction was severe in low concentrated formocresol and the (higher the concentration of formocresol the milder the inflammatory reaction was more evident. 2. In zinc oxide group, inflammatory change was milder at 3 weeks than 1 week, and proliferation of young connective tissue was seen at 3 weeks. 3. In calcium hydroxide group, inflammatory change in relation to the concentration of formocresol was not noticeable. 4. In calcium hydroxide group, repair process with decreased inflammatory reaction and fibrosis, and dentin bridge like layer was found at 3 weeks.