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박근형,김선재,정지흔 한국식생활문화학회 1992 韓國食生活文化學會誌 Vol.7 No.1
Jindo Hongju is a traditional liquor in Jindo island of Korea. The characteristics of Hongju are its unique flavour by fermetation and red color of gromwell(Lithospermum erythrorhizon) root. However, the evaluation of red pigment is different from one manufactures to other manufatures and from place to place, also the standard method is not established. An attempt has made to compare the quality of gromwell root from different places and to standardize the extracting method. The results obtained from this study are summerized as follow, The chemical properties and composition of gromwell root from Jindo and other areas were compared. There were no difference among the samples in moisture content, content of naphtoquinone derivatives and absoption spectra. These results indicate that the pigments from Jindo and other region products seems to be the same quality. For efficient extraction of gromwell pigment, more than 40% ethanol as solvent and at least 10 hours extraction time was required. According to the visual test for Hongju pigment, the most preferable color was that it shows absorbance of 1.0 (contents of shikonin was 3.90 ㎎/45% EtOH 20 ㎖). From this visual test it can be proposed that the may be applied absorbance at 1.0 for the quality control of pigment.
균근균(菌根菌) , Pisolithus tinctorius 가 생산하는 Gibberellin 양 활성
박근형,이종욱,김동연,정지흔,김관,강성훈 한국농화학회 1984 Applied Biological Chemistry (Appl Biol Chem) Vol.27 No.1
Experiments on the GA production ability by ectomycorhizal fungus, Pisolithus tinctorius was carried out to investigate specific physiological phenomena of growth increase in host plants by formation of mycorrhizae, The culture extract of P. tinctorius was purified by solvent fractionation, sephadex LH-20 chromatography, silica gel partition chromatography and TLC, successively. GA activities in the purified GA fractions were monitored by micro-drop bioassay using dwarf rice seedlings, $quot;Tan-ginbozu$quot;. 30∼60% EtOAc election fractions of silica gel pardon chromatography and the zone of Rf 0.1∼0.4, 0.6∼0.8 of TLC exhibited the GA-like activities. The GA activities were increased with the more treated amount of culture extracts. This activity in 100㎖ of culture solution was equivalent to 0.1ng of GA₃.
잠두(Vicia faba L)가 생산하는 Brassinisteroid 활성물질
박근형,현규환,Park, Keun-Hyung,Hyun, Kyu-Hawn 한국응용생명화학회 1987 한국농화학회지 Vol.30 No.1
미숙잠두 종자의 추출물을 silica gel chromatography, Sephadex LH-20 chromatography, TLC, 역, 순상의 HPLC 등에 의해 분획정제하여 BR 활성물질을 한국산 벼를 이용한 생물검정법에 의해 검색한 결과, 2종의 BR의 존재가 인정되었으며, BR 보다 분자량과 극성이 작은 물질의 존재가 시사되었다. Occurrence of two type brassinosteroid-like substances and less molecular weight substance than brassinosteroid were suggested in immature Vicia faba seeds extracts, which were purified using various chromatographic techniques, by Korean rice-lamina inclination test.