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류준형,남윤권,공승표 한국수산과학회 2015 Fisheries and Aquatic Sciences Vol.18 No.1
We investigated factors affecting primary cultures of Pacific abalone Haliotis discus hannai ovary-dissociated cells to identify general aspects of their early-phase culture. Ninety-seven cell populations derived from 30 individuals were cultured in different media with varying compositions of medium supplements, and initial attachment, subculture, and survival for ≥10 weeks were assessed according to medium composition and individual. We also examined the time required for subculture and the rate of cell death according to both culturing period and passage number within 10 weeks. A lack of fetal bovine serum (FBS) and hemolymph significantly inhibited the growth of cultured cells, while we detected no significant effect of medium composition on initial cell attachment. Through data reallocation, with the omission of data from cell populations cultured in FBS-free and hemolymph-free media, we showed that growth inhibition was also affected by individual differences among the abalones used. During the culture, we observed four different types of cell morphology. Moreover, considerable time was required for subculture—18.4 and 19.5 days for first and second subcultures, respectively—and cell death did not occur within 30 days or for passage 0. Our results will provide valuable information for developing universal cell culturing guidelines in abalone species and suggest the feasibility of culturing abalone ovary-dissociated cells.
류준형 중국고중세사학회 2023 중국고중세사연구 Vol.67 No.-
Dongguanzouji is the work of Pei Ting Yu, who had lived between the end of Tang Dynasty and the period of Later Liang Dynasty. Although it is difficult to determine the exact date of writing, it is generally assumed that it was completed around the second year of Dashun(891) and presented the following year in the first year of Jingfu(892). The purpose of the writing was that Pei Ting Yu participated in compiling the annals of the three emperors during the reign of Xuanzong, Yizong, and Xizong, but when the writing of the annals failed, he collected historical materials from the time of Xuanzong and presented it to Zhaozong instead. This leads us to guess the characteristics of Dongguanzouji as an annal record, that is, as a short-term record of the public year of Xuanzong’s reign. The third volume of Dongguanzouji consists of a total of 31 items. The length of each item is 51 characters in the shortest case and 316 characters in the longest case. Fifteen items, half of the 31 items, are concentrated in the 12th and 13th years of Dazhong period, that is, at the end of Xuanzong’s reign, confirming the lopsidedness of the records. In terms of content, each item is composed of an independent form with no interrelationships. The content related to the emperor and the content related to a specific individual consisted of about half of each with 13 items. In terms of historical value, several characteristics can be identified. First of all, 24 items are confirmed only in the Dongguanzouji or considered as the earliest records among the other historical records. This is more than 75% of the entire second volume, which clearly shows the historical value of the third volume of Dongguanzouji. The third volume of Dongguanzouji can be highly valued in that it is the only historical record focusing on Dazhong period. In addition, it is worth referring carefully to the fact that it is the only record that is not confirmed in other historical sources, as well as the fact that it has become a reference book for historical sources compiled later.