악교정수술을 받은 III급 부정교합자의 치료 전, 후의 하악치열궁 변화

남형진(Hyung-Jin Nam),손우성(Woo-Sung Son),박수병(Soo-Byung Park),김성식(Seong-Sik Kim) 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.4

본 연구는 악교정수술을 받은 골격성 III급 부정교합자의 치료 전, 후의 하악치열궁 변화를 관찰하고, 이들이 초진시의 측모두부방사선 규격사진 및 석고모형과 어떠한 상관성을 가지고 있는지를 알아보기 위해 골격성 III급 부정교합자로 진단받은 31명(남자 14명, 여자 17명, 평균 21.8 ± 3.3세)을 대상으로 치료 전, 악교정수술 전, 악교정수술 후 채득한 측모두부방사선 규격사진과 치료 전, 후의 석고모형을 계측하여 상관분석과 회귀분석을 시행하였다. 연구 결과 Mn-LMMC, Mn-LIE, Mn-MnOcc는 술전교정치료에 의한 유의한 차이를 보이지 않았다. 치료 전의 계측치와 치료 후 변화된 계측치와의 상관관계에서는 치열궁공간부족(ALD)은 ICW, IPW1의 변화량과 순상관관계(r = 0.492, 0.615)를 보였으며, 치열궁장경(arch length)의 변화량과도 순상관관계(r = 0.641)를 보였다. 그러나 Spee 만곡은 전치부 치축 각도 변화와 치열궁 폭경 및 장경 등 각각의 변화량과는 상관관계를 보이지 않았다. 이를 이용하여 회귀분석을 시행한 결과, 술전교정 후 Arch length, IPW1의 변화량은 치료 전의 계측치로 각각 64.0%, 75.8%를 설명할 수 있었다. 이상의 연구에서 골격성 III급 부정교합자의 치료 전, 후의 변화량과 치료 전 측모두부방사선 규격사진 및 석고모형 간에 관련성이 있었고 악교정수술을 동반한 교정치료 계획을 수립할 때 술전교정치료의 목표 설정과 악교정수술 결과의 예측에 도움이 될 것으로 판단된다. Objective: The purpose of this study was to investigate changes in the mandibular dental arch from presurgical orthodontic treatment and orthognathic surgery, and to evaluate the relationships between the pretreatment records and changes of mandibular dental arch in skeletal Class III malocclusion individuals. Methods: Lateral cephalometric radiographs and mandibular study models of 31 adults with skeletal class III malocclusion were taken and measured. All measurements were evaluated statistically by ANOVA, Scheffe`s Post Hoc, and paired t-test, and correlation coefficients were evaluated. Results: No significant difference in Mn-LMMC, Mn-LIE, Mn-MnOcc was detected between pretreatment and presurgical groups. Statistically significant but low correlations were demonstrated between the initial arch length discrepancy (ALD) and change in ICW, IPW1 (r = 0.492, 0.615) and change in arch length (r = 0.641). No association was seen between the initial depth of curve of Spee and change in mandibular incisor angle and arch width or arch length. Regression analysis showed that the amount of change for arch length and IPW1 could be explained by 64.0% and 75.8% of the pretreatment variables respectively. Conclusions: This study suggests that orthognathic surgery results can be predictable by measuring the pretreatment records.

형질전환된 식물세포에서 고정화 방법을 통한 hCM-CSF의 생산성 증대 연구

노윤숙(Yun-Sook Noh),남형진(Hyung-Jin Nam),최홍열(Hong-Yeol Choi),탁사라(Sa-Ra Tak),김동일(Dong-Il Kim) 한국생물공학회 2015 KSBB Journal Vol.30 No.2

Plant cell immobilization can protect plant cells from shear forces and increase the stability of gene. An additional advantage of immobilization is the easiness for performing continuous culture with cell recycling. Therefore plant cell immobilization can overcome the limitations of plant cell applications. In addition, target protein should be selected from pharmaceutical proteins to get rid of low expression level problem. The enhanced production of human granulocyte-macrophage colony-stimulating factor (hGMCSF) was investigated in immobilized Nicotiana tabacum suspension cell cultures. When the cells were immobilized in polyurethane foam, specific production of hGM-CSF was higher than that in alginate bead immobilization. Optimum continuous culture condition was the addition of 60 g/L sucrose in growth media with exchanging media every 6 day. Under the same condition, specific hGM-CSF production was 7 times higher in a 500-mL spinner flask than that in 100-mL Erlenmeyer flasks. Therefore, development of an effective immobilization process would be possible when the advantage of easy cell recycling was used. Consequently, enhanced production of target proteins could be possible in immobilized continuous cultures when the advantages of immobilization were applied.

형질전환 벼 현탁세포 배양에서 세포 사멸 억제를 통한 hCTLA4Ig 생산성 증대

김명식(Myong-Sik Kim),남형진(Hyung-Jin Nam),김민섭(Min-Sub Kim),권준영(Jun-young Kwon),김동일(Dong-Il Kim) 한국생물공학회 2013 KSBB Journal Vol.28 No.4

Transgenic plant cell cultures are an attractive expression system for the production of industrial and pharmaceutical proteins because of their advantages in safety and low production cost. Human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) was produced and secreted when sugar was depleted in culture medium by transgenic rice cell lines (Oryza sativa L.) using RAmy3D promoter. Due to the production of the target protein by sugar depletion, concomitant occurrence of cell death is inevitable. For that reason, inhibition of cell death for enhancing productivity was necessary for the production period without energy sources. Supplementation of 0.1 mM sodium nitroprusside improved cell viability by 1.4-fold and maximum hCTLA4Ig production by 1.3-fold compared to those of control. Addition of 1 and 10 mM glutathione, N-acetylcysteine (NAC), and nicotinamide inhibited apoptotic-like programmed cell death by decreasing the activity of reactive oxygen species. Production hCTLA4Ig was enhanced 1.4-, 1.25-, and 1.15-fold with 10 mM NAC, 1 mM NAC, and 1 mM glutathione, respectively. In addition, it was found that the supplementation of NAC enhanced the cell viability.

Sodium butyrate와 sodium pyruvate 첨가에 의한 hCTLA4Ig 생산성 증대

유미희(Mi-Hee Yoo),김수진(Soo-Jin Kim),권준영(Jun-Young Kwon),남형진(Hyung-Jin Nam),김동일(Dong-Il Kim) 한국생물공학회 2011 KSBB Journal Vol.26 No.5

Human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig), an immunosuppressive agent, was expressed in transgenic rice cells using RAmy3D promoter and RAmy1A signal peptide for the inducible production and secretion into culture media by sugar depletion. In this study, sodium butyrate was used as a small molecular enhancer (SME) to enhance the production of hCTLA4Ig in transgenic rice cell suspension cultures. When 1 mM sodium butyrate was added in sugar-free media, relative viability was not reduced, while the productivity was improved 1.3-fold. In addition, by supplementing 87 mM sodium pyruvate as an alternative energy source during the production phase, death rate of the cells was decreased. When sodium pyruvate was not added, most cells became dead at day 6. However, by adding sodium pyruvate, 18% of viability can be maintained until day 10 and the production of hCTLA4Ig was enhanced 1.4-fold. When the combination of sodium pyruvate and sodium butyrate at optimum concentrations was added, the highest viability and hCTLA4Ig production could be obtained. The highest level of hCTLA4Ig reached up to 35 mg/L at day 10.

형질전환 벼 현탁세포 배양에서 hGM-CSF의 in situ Recovery 연구

명현종(Hyun-Jong Myoung),최홍열(Hong-Yeol Choi),남형진(Hyung-Jin Nam),김동일(Dong-Il Kim) 한국생물공학회 2015 KSBB Journal Vol.30 No.3

Production of foreign proteins by transgenic plant cell cultures has several advantages such as post-translational modification, low risk of product contamination and low-cost production and purification. However, target proteins are degraded by extracellular proteases existing in the media. A solution to this problem is the use of perfusion culture and ion exchange chromatography for the application of integrated bioprocess using in situ recovery. With this method, production of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) was investigated in this study. First, optimization of cell concentration during the induction phase for the production of hGM-CSF was examined. As cell concentration increased, the level of hGM-CSF was decreased due to the presence of extracellular proteases. Induction using sugarfree media produced 33% more hGM-CSF. The effects of pH on the binding of hGM-CSF to cationic and anionic exchange resins were also investigated. In terms of stability, optimal pH was found to be 5~7. In the case of using buffer exchange when CM-Sepharose was used as a cationic exchange resin, optimal pH for binding was 4.8 and adsorption yield was 77%. When DEAE-Sepharose was used as an anionic exchange resin, it was 5.5 (74%). Without buffer exchange, optimal pH was 4.6 (84%). From these results, an integrated bioprocess using in situ recovery with simultaneous production and separation of foreign protein in transgenic plant cell suspension cultures was found to be feasible.

형질전환 벼 현탁세포 배양에서 혼합효율과 조정배지가 hCTLA4Ig 생산에 미치는 영향

최홍열(Hong-Yeol Choi),박준용(Jun-Yong Park),남형진(Hyung-Jin Nam),공미경(Mi-Kyung Gong),유예리(Ye-Ri Yoo),김동일(Dong-Il Kim) 한국생물공학회 2015 KSBB Journal Vol.30 No.6

Transgenic rice cells using RAmy3D promoter can provide high productivity, and the production of recombinant protein is induced by sugar starvation. In this system, productivity was reduced during the scale-up processes. To ensure the influences of shear stress and oxygen transfer rate, working volume and mixing performances were investigated under various agitation speeds and working volumes. In addition, inoculation methods including suspended cells and filtered cells were compared. Working volumes and shaking speeds were 300, 450 mL and 80, 120 rpm, respectively. Hydrodynamic environment of each condition was measured numerically like mixing time and kLa. Good mixing performance and high shear stress were measured at high agitation speed and low volume. The highest level of hCTLA4Ig was 30.7 mg/L at 120 rpm, 300 mL. When conditioned medium was used for inoculation, increased cell growth was noticed during the day 0~4 and decreased slower than filtered cells. Compared with filtered cells, the maximum hCTLA4Ig level reached 37.8 mg/L at 120 rpm, 300 mL and lower protease activity level was observed. In conclusion mixing performance is critical factor for productivity and conditioned medium can have a positive effect on damaged cells caused by hydrodynamic shear stress.

골격성 Ⅲ급 부정교합자의 전치부 수직피개도에 영향을 미치는 요소 - 개교군과 피개군간의 비교

정정화,남형진,강상욱,손우성 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25

맹출장애가 있는 하악대구치의 임상적 관리

손우성,이해경,남형진,강상욱,송효경,김성식 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25