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RISS 인기검색어
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- 저자 : 나석한 ( Seuk Han Na ) (검색결과 3 건)
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나석한,최성현,랜친핸드,배형철,남명수,Na, Seuk-Han,Choi, Seong-Hyun,Renchinthand, Gereltuya,Bae, Hyoung-Churl,Nam, Myoung-Soo 한국축산식품학회 2008 한국축산식품학회지 Vol.28 No.3
This study was carried out to assess the fermentation properties of lactic acid bacteria (LAB) isolated from bovine colostrum and effects of feeding fermented colostrum feed on the growth to piglet. A total of 427 colonies were isolated from bovine colostrum on the BCP plate count agar. These LAB isolated were subcultured in 10% reconstituted skim milk, and seven strain thereof were selected for their highest acid productions, and two strain thereof were finally selected for their excellent sugar utilization. These strains were identified as Streptococcus thermophilus and Streptococcus macedonicus based on l6S rDNA sequencing data, named S. thermophilus CNB-11 and S. macedonicus CNB-11 respectively. For fermentation profiles, sugar utilization, acid production and viable cell counts were excellent in S. thermophilus CNB-11 as compared with S. macedoniclts CNB-11 after 48 hour. The effect of feeding fermented colostrum feed 0.5% using S. thermophilus CNB-11 was investigated for growth rate, analysis of blood and incidence of diarrhea. 24 heads of piglets were divided into two groups: the experimental and the control of 12 animals each. The average growth rate in the pigs fed fermented colostrum feed was higher 16.73% compared with control diet (p<0.05). There were no differences in the concentrations of blood glucose, cholesterol, albumin and globulin in pigs fed fermented colostrum feed as compared with control piglets. Incidence of diarrhea was no in pigs fed fermented colostrum feed as compared with control piglets.
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배형철,랜친핸드,나석한,최성현,남명수,Bae, Hyung-Churl,Renchinthand, Gereltuya,Na, Seuk-Han,Choi, Seong-Hyun,Nam, Myoung-Soo 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.4
Questionnaire on utilization of colostrum was carried out in 33 farms. Highest milking cow numbers were 20-40 heads in 45.5% of the farms. Calf numbers were 20-40 heads in 39.4% of the dairy farms and 40-60 heads in 42.4% of the farms. The amount of colostrum production per day was 20-30 kg in 30.3% of the farms and 30-40 kg in 30.3% of the farms. Colostrum intake per calf per day was 4 kg in 45.5% farms. Surplus colostrum after intake by calf was farms (85%) was wasted in 67% of the farms. More than 90% of dairy farms recognize an necessity to use surplus colostrum. Selling price of surplus colostrum was below 1,000 won per kg at 15 dairy farms and 1,000-2,000 won per kg at 4 dairy farms. Colostrum collection and use should be done by the dairy farms of antibiotics free. Quality control of colostrum should be done by testing antibiotics, microbacterium, chemical composition, somatic cells and etc. The colostrum is subjected to cool down below $4^{\circ}C$ just after milking and process for the colostrum products.
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기타 : 맥아에서 분리한 Bacillus amyloliquefacies CNL-90이 생산하는 α-amylase와 Protease의 특성
최성현 ( Seong Hyun Choi ),남명수 ( Myoung Soo Nam ),배형철 ( Hyoung Churl Bae ),나석한 ( Seuk Han Na ) 한국동물자원과학회 ( 구 한국축산학회 ) 2012 한국축산학회지 Vol.54 No.2
Bacillus 균주가 생산하는 효소를 식품과 동물사료 첨가제로 이용하기 위해서 맥아로부터 단백질 분해능력과 전분 분해능력이 우수한 균주를 분리 동정하여 Bacillus amyloliquefaciens CNL-90으로 명명하였다. 분리된 B. amyloliquefaciens CNL-90이 생산하는 α-amylase의 안정성은 pH는 7, 온도는 40℃, protease의 경우는 pH가 7, 온도는 50℃에서 안정했다. B. amyloliquefaciens CNL-90을 밀기울에 접종하여 고체 배양한 결과 α-amylases의 효소활성은 6일 배양 후 290,000 unit/kg이었고, protease의 효소활성은 310,000 unit/kg으로 나타났다. 밀기울에 고체 배양한 생균수는 배양 6일 후에는 2.2×109CFU/g으로 높았다. 사료 요구율 개선은 B. amyloliquefaciens CNL-90 배양액 0.2% 첨가구가 대조구에 비해서 일당 증체량은 6.66% 높았고, 사료 요구율은 0.05% 효과가 있었다. 이러한 결과는 B. amyloliquefaciens CNL-90이 생산하는 단백질 분해효소와 전분 분해효소는 식품산업 및 가축사료첨가제 산업에 응용할 수 있는 가능성을 확인하였다. A bacterium, identified as Bacillus amyloliquefacies CNL-90 using 16S rDNA analysis, was isolated from malt grain. The optimal activities of its α-amylase and protease were observed at pH 6 and 60℃, and at pH 6 and 50C, respectively although their activities remained stable at pH 7 and 40℃ for α-amylase and at pH 7 and 50℃ for protease. After solid-state fermentation of B. amyloliquefacies CNL-90 on wheat bran for 72hr or 144hr, the α-amylase and protease activities were 170,000 and 290,000 units/kg, and 290,000 and 310,000 units/kg, respectively. The viable bacterial cell counts were 1.5×109 CFU/g and 2.2 × 109 CFU/g at 72hr and 144hr of the solid-state fermentation, respectively. A feeding trial with a total of 127 piglets was also conducted. The animals were divided into two groups: an experimental group fed with the fermented product(63 piglets) and a control group(64 piglets). The growth rate of the experimental group was 6.66% higher than that of the control group(P<0.05). The results of this study indicate that the α-amylase and protease from B. amyloliquefacies CNL-90 can be used for industrial applications due to their activity in production of carbohydrate hydrolysates.
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