황칠나무의 칠액 분필 촉진

김세현,정남철,나천수,김삼식 ( Sea Hyun Kim,Nam Chul Jung,Chun Soo Na,Sam Sik Kim ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.2

The stimulated secretion of golden varnish sap by the infection of microorganisms on the bark of Dendropanax morbifera Lev. was observed in the natural habitats. Four candidate strains of such microorganisms(i.e., Sb1, Sb2, Sb3 and Sb4) were isolated from the bark of D. morbifera which was infected in natural. Of them, Sb3 and Sb4 strains showed stimulated secretion of golden vernish sap with the greatest stimulation by Sb4 strains. The yield of golden varnish sap from the bark of D. morbifera was boosted about 3.4--7.7 times by the inoculation of Sb4 strain in compassion with that collected from lacquer tree(Rhus verniciflua Stokes) by the traditional method. The mycelium color of Sb4 strain was grayish white. The optimum growth temperatures and pH were ranged from 30 to 35℃ and from pH 6.5 to 7.0.

다래 및 교잡종다래 꽃의 형태적 특성과 유연관계

황석인(Suk-In Hwang),장용석(Yong-Seok Jang),김만조(Mahn-Jo Kim),김세현(Sea-Hyun Kim),박영기(Youngki Park) 한국양봉학회 2010 韓國養蜂學會誌 Vol.25 No.4

To examine the taxonomic relationships among 6 cultivars by 14 flower morphological characters, principal component analysis and cluster analysis were carried out. In this study, we analyzed the flower morphological characteristics of three new hadry kiwi (Actinidia arguta) cultivars and three new hybrid kiwi (A. arguta×A. deliciosa) cultivars to investigate the species classification and the potential for using honey plants. As result of principal component (PC) analysis using 14 variables from 6 cultivars, eigen value of the first principal component was 8.9054 and the first two PCs was accountable for 74.00% of the total variance. The first PC was correlated with those characteristics that were mainly related to the color of width of inflorescence, color of petal a, length of pedicel and no. of stamen. From the cluster analysis using UPGMA method, we can assumed that six cultivars could be clustered into two groups. Group Ⅰ comprises three A. aruguta, Sea-Han, and Dea-Sung, Chil-Bo, and the other one, Group Ⅱ consists of three hybrid kiwi, Dea-Myng, Hwang-Oak, and Ja-Wang.

밤나무 주요 4품종의 개화 및 화밀 특성 분석

김세현(Sea Hyun Kim),이안도성(Andosung Lee),권해연(Hae Yun Kwon),이욱(Uk Lee),김문섭(Moon Sup Kim) 한국양봉학회 2017 韓國養蜂學會誌 Vol.32 No.3

In order to provide a basic data of Castanea spp. as a honey plant, flowering time and nectar characteristics were investigated using Korean prevailing chestnut cultivars: ‘Daebo’, ‘Okkwang’, ‘Riheiguri’ and ‘Tsukuba’ planted in clonal archive in Hwaseong city. There were differences among four cultivars in the beginning of flowering and the end of the flowering. In 2015, female flower bloomed approximately eight days earlier than the male flower. Also, there were differences among four cultivars in nectar volume per catkin as the values were 54.3μl and 6.7μl in ‘Okkwang’ and ‘Daebo’, respectively. On the contrary, in the sugar content, the values were 15.6μg/μl and 69.3μg/μl in Okkwang and Daebo, respectively. Therefore, it is possible to assume the sugar content per catkin using the total nectar per catkin and the total amount of free sugars. Based on the formula, we estimated the values: Tsukuba was 1401.6μg; Okkwang was 777.5μg; Riheiguri was 708.9μg; and Daebo was 486.4μg. Moreover, we investigated the amino acid content. The result was that proline, glutamic acid, asparagine, and alanine were major amino acids in four cultivars. Also, C. spp. showed different values in many aspects such as catkin morphology, nectar characteristics, sugar content, and amino acid content. The sugar content per catkin which determines a honey yield considered, it is believed that Tsukuba is highly valued as a honey tree. It would be needed to further study related to the nectar characteristics of female flower to exactly estimate the total nectar volume per tree and the total honey yield per tree.

팥배나무 선발집단의 입지환경과 식생구조 분석

김세현(Sea Hyun Kim),남재익(Jea Ik Nam),김영기(Young Ki Kim),김문섭(Mun Seop Kim),송정호(Jeong Ho Song),박인협(In Hyeop Park),고영석(Young Seok Go) 한국양봉학회 2019 韓國養蜂學會誌 Vol.34 No.3

Sorbus alnifolia (Siebold & Zucc.) K. Koch were usually distributed in the middle or the top of the mountain under 1,100 m of sea level as a small population. Based on physiognomy, the predominant species in the natural populations of S. alnifolia can be summarized as follows; Quercus aliena-S. alnifolia in Mt. Mani; Q. varirabilis-S. alnifolia in Mt. Bawang and Mt. Geumo; S. alnifolia in Mt. Gaji, Mt. Duryun and Wando. On the other hand, in the case of Anmyundo population, there were no predominant species and S. alnifolia were growing with Q. serrata, Q. varirabilis, Pinus densiflora and P. thunbergii. The four species (Q. serrata, Prunus sargentii, Lindera obtusiloba and Smilax chian) appeared in all the natural populations of S. alnifolia. The sums of important values of S. alnifolia for each layer were 230.3% in tree layer, 124.3% in subtree layer, and 9.1% in shrub layer, respectively. The Morista’s indexs of S. alnifolia were 1.12 in tree layer, 1.00 in subtree layer, and 0.28 in shrub layer, respectively. This result shows that S. alnifolia occurs randomly in tree and subtree layer but regularly in shrub layer. A total of 91 species was found in all the surveyed plots. Species diversity of S. alnifolia populations ranged from 0.78 to 1.30. Evenness and dominance showed the ranges of 0.75~0.89 and 0.11~0.25, respectively. Cluster analysis based on the species composition showed that Wando, Mt. Duryun, Mt. Gaji, and Mt. Mani composed one group and Anmyundo, Mt. Geumo and Mt. Balwang made up the other group.

헛개나무 선발개체의 특성과 꿀벌 방화

김세현(Sea-Hyun Kim),정헌관(Hun-Gwan Chung),한진규(Jingyu Han) 한국양봉학회 2005 韓國養蜂學會誌 Vol.20 No.1

This study was estimate and analysis about characteristics of flowering, fruit and foraging of honeybee from 70 clones of Hovenia dulcis var. koreana Nakai. that trees cultivated for superior tree selecting. The results obtained from this study can be summarized as follows. 1. 9 clones showed over 1,000 flowering among flowered 50 clones. Flowering period was 19 days from 17 June to 5 July. Flowering peak appeared 25 June and that showed 25% flowering rate. 2. In the fruiting characteristic analysis, total means of the Diameter of fruiting lateral(DFL), Number of total flowering lateral(NTFL), Number of fructify lateral (NFL), Rate of fructify lateral(RFL), Number of average bunch per fructify lateral(NABFL), and Number of average bunch per fruiting lateral(NABPFL) were 1.57㎝, 14.5, 12.5, 82.56%, 3.23 and 39.18, respectively. Especially, the range and coefficient of variation for the NTFL and NFL, NABFL and NABPFL characteristics were 3~60(CV=57.8%) and 1-60(CV=69.0%), 1~28(CV=56.0%) and 7.3~91.6 (CV=61.2%), respectively. Among Selected 10 clones which had major fruiting characteristics, GW 3-3, GW 1-17 and GW 1-9 showed 91.68, 86.00 and 63.30 for NABFL, respectively. Also, Ranges of Weight of fruit petiole per individual(WFPI) and Yield of individual(YI) were 3.9~4.7㎏ and 9.43~11.26㎏, respectively in GW 3-3, GW 2-5 and GW 1-17. 3. Results of annual flowering characteristic from 2000 to 2005 showed three years cycle for flowering rate. 4. Honeybee foraging started 17 June and ended 5 July in flower of Hovenia dulcis var. koreana Nakai. The most number of honeybee foraged on 25 June and that number was 226.3. Foraging of honeybee was more influenced by precipitation than temperature. Also, Foraging started 8 o'clock and showed peak 12~13 o'clock and decreased after peak time and ended 19 o'clock in results of foraging estimate by hour.

복분자딸기 클론별 과실특성과 항산화 활성 탐색

김세현 ( Sea Hyun Kim ),정헌관 ( Hun Gwan Chung ),장용석 ( Yong Seok Jang ),박영기 ( Young Ki Park ),박형순 ( Hyung Soon Park ),김선창 ( Sun Chang Kim ) 한국산림과학회 2005 한국산림과학회지 Vol.94 No.1

Fruit characteristics, saccharinity, and antioxidalive activity of selected clones of Rubes coreanus, grown in Korea, well-known as edible and medical resources, frequently used and which has an excellent pharmacological activity are examined and analyzed. The results obtained from this study can be summarized as follows; Result of surveying characteristics for the fruit and saccharinity variation by collection time, there are some meaningful differences between examined clones and the collection time, exception of the fruit width. From the clones, SG 3 is the biggest in size, the highest in the saccharinity out of five clones, on the other hand, CJ 18 and SH 3 are the opposite. Meanwhile, comparing examined saccharinity factor with maximum temperature and precipitation out of daily climates at Suwon Meteorological Observatory at the same period, on the day the highest temperature is 29.5℃, saccharinity distinctively ascends, but on the day the precipitation is recorded 121.5 mm and next day. the saccharinity descends. Also, five-day saccharinity until the precipitation lower than 0.1 mm per day shows from 10,4 to 11.5 Brix higher than the average saccharinity of 9.7 Brix. Fifteen clones including CA 6 about fruit characteristics in terms of collection layer and saccharinity variation were examined, there is significantly difference among examined clones. but about the collection layer, only saccharinity factor has meaningful significance. For clones, MJ 11 is the biggest and excellent character of 15 clones but the saccharinity, 10.3 Brix lows a bit, MC 9 and HAE 5 are the worst fruit in character but saccharinity is 13.4 Brix and 10.6 Brix which is higher than the average saccharinity of 9.8 Brix. About saccharinity for the collection layer, the fruit average saccharinity, collected from the upper layer which is good at lightness and temperature condition, is 10.2 Brix which shows higher than the one of the fruit, 9.7 Bix and 9.5 Brix collected from the middle or the lower layer. The saccharinity of fruit collected from the middle or the lower layer is lower than the average saccharinity of 9.8 Brix out of 15 surveyed clones. Surveying antioxidative activity resulted from free radical scavenging activity about 30 clones of fruit in total, there is difference between surveyed clones. Especially, Tmmatured fruit extracts exhibited higher free radical scavenging activity of 94.5 to 97.5% which indicates the similarity to Ascorbic acid, unlike other clones, on the concentration of 250 to 1,000 ppm.

헛개나무 추출물의 화장품 생리활성에 관한 연구

김세현 ( Sea Hyun Kim ),전동하 ( Dong Ha Jun ),장민정 ( Min Jung Jang ),이진태 ( Jin Tae Lee ),이창언 ( Chang Eon Lee ),한진규 ( Jing Yu Han ),김진철 ( Jin Chul Kim ),이도형 ( Do Hyung Lee ) 한국산림과학회 2010 한국산림과학회지 Vol.99 No.6

Hovenia dulcis var. koreana Nakai has been reported to liver function improvement effect as functional materials for food and medicine. On these facts, biological activity and safety test were conducted to evaluate biological activities of the fruit petiole and root extracts of H. dulcis as a potential cosmeceutical ingredient. Cosmeceutica activities of different extracts were examined by l.l-diphenyl-2-picrylhydrazyl (DPPH) radical generation, the ABTS+ cation decolorization, tyrosinase activity, collagenase activity and elastase activity compared with the properties of the commercial antioxidant butylated hydroxytoluene (BHT) and L-ascorbic acid(AA). The antioxidant activities HDFW, HDFE, HDRW and HDRE were 83.6%, 39.6%, 85.9% and 74.5% in DPPH assay, 99.5%, 13.7%, 96.4% and 88.6% in ABTS assay. Tyrosinase inhibitiory activities HDFW were 56.0% at 1,000 ppm. Measured the inhibition effect of the H. dulcis about collagenase and elastase where break the peptide bonds in collagen and enzyme from the class of proteases where exists in the dermis. The H. dulcis was inhibition the two kind enzymesm, collagenase activities being on a high scale inhibition, was same concentration. Uses the anti oxidation effect and a anti-wrinkle effect of this resultant H. dulcis and with the functional cosmetics use is thought with the fact that will be possible.

돌배나무류 과실의 에탄올 추출물의 페놀성 화합물 함량 분석

김세현 ( Sea Hyun Kim ),심상희 ( Sang Hee Shim ),김혜수 ( Hyeu Soo Kim ),송정호 ( Jeong Ho Song ),김문섭 ( Moon Sup Kim ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-

돌배나무류( Pyrus spp.)는 장미과(Rosaceae)에 속하는 낙엽활엽수로써, 열매는 식용 뿐만 아니라 해열, 건위, 지갈, 이뇨, 항당뇨 등의 효능이 있어 민간약으로 널리 사용되어왔다. 돌배나무류는 돌배나무( P. pyrifolia (Burm. f.) Nakai), 산돌배( P. ussuriensisMaxim.), 백운배나무( P. ussuriensisvar.hakunensis (Nakai) T. B. Lee), 정선배나무( P. pyrifolia (Burm. fil.)(Jeongseon)), 청실배나무( P. ussuriensis Max. var. ovoidea Rehder) 등 종류가 다양함에도 불구하고 수종별 생리활성 및 성분 연구는 미비하다. 본 연구에서는 돌배나무류의 다양한 열매를 이용하여 기능성 소재를 발굴하기 위하여 생리활성 성분을 비교·분석하였다. 실험에 사용된 HPLC는 Agilent사의 1200 series를 사용하였고, column은 Luna C18(10.00×250mm, 5μm, Phenomenex)을 사용하였으며, 검출기는 UV 280nm, 이동상으로는 0.1% TFA를 첨가한 acetonitrile과 0.1% TFA를 첨가한 물을 사용하여 gradient profile로 하였고, 유속은 2.0ml/min으로 하였다. 돌배나무류 70% 에탄올 추출물을 profiling한 결과, phenol성화합물 중 arbutin과 chlorogenic acid가 주로 함유되어 있었다. 특히, chlorogenic acid의 함량(2.48~14.36mg/g)이 arbutin(1.02~7.83mg/g)보다 더 높은 것으로 확인되었으며, chlorogenic acid와 arbutin의 평균함량은 각각 돌배나무가 6.8, 2.8mg/g, 산돌배가 7.6, 3.5mg/g, 정선배나무가 3.3, 1.4mg/g, 백운배나무가 10.5, 2.6mg/g, 청실배나무가 3.8, 1.0mg/g로 수종별로 상이하게 나타났다. The Korean wild pears have been considered not only fruit but an herbal medicine in East Asia. The fruit of this plant, known as a pear, is common and highly consumed in Korea. Although varieties of Pyrus were various, the study about bioactive substance and bioactivity of Pyrus varieties was incomplete. The study aims to assess bioactive substance of 70% ethanol extracts of 5 wild pear species in Korea and its potential as a medicinal ingredient of functional food and medicine. The used HPLC system was a Agilent 1200 series(Agilent, USA) equipped with a Luna C18 Column(10.00×250mm, 5μm, Phenomenex), coupled with a UV detector(280nm). The mobile phase consisted of 0.1% TFA in actonitrile, water at a flow rate of 2.0ml/min. The result of profiling HPLC chramatogram from 70% ethanol P. spp. extracts showed that the major phenolic compound was found as chlorogenic acid, arbutin. In the 70% ethanol extracts of P. spp., chlorogenic acid(2.48~14.36mg/g) was more than arbutin(1.02~ 7.83mg/g) and the chlorogenic acid and arbutin content were significant differences among the P. pyrifolia (Burm. f.) Nakai(6.8, 2.8mg/g), P. ussuriensis Maxim.(7.6, 3.5mg/g), P. pyrifolia (Burm. fil.)(Jeongseon)(3.3, 1.4mg/g), P. ussuriensis var. hakunensis (Nakai) T. B. Lee)(10.5, 2.6mg/g) and P. ussuriensisMax. var. ovoidea Rehder(3.8, 1.0mg/g).

음나무 종자의 저장방법 및 GA3 농도별 발아특성

김세현 ( Sea Hyun Kim ),이철호 ( Cheol Ho Lee ),정헌관 ( Hun Gwan Chung ),장용석 ( Yong Seok Jang ),박형순 ( Hyung Soon Park ) 한국산림과학회 2003 한국산림과학회지 Vol.92 No.3

This study was conducted to reveal the effective germination method of K.septemlobus by storage conditions. GA_3 concentrations, germination temperatures, and seed collection times The obtained results are shown below; The rate of spilt achene pericarp was analyzed for 6 storage methods; i.e. dry and moist room temperature storage, dry and moist cold storage, moist storage and cold stratification storage until 12 weeks after sowing. The moist storage method showed the most excellent value(82%) in the rate of split achene pericarp over all storage methods. But, non-split achene pericarp occurred when the seeds were stored either dry room temperature or at 4`E without providing any moisture up to 12 weeks after sowing. Germination rate was peak(80%) when treated with 1,000mg/ℓ GA_3 after gradual exposure to the temperature ranging from 4℃ to 25℃ with moisture When the seeds were stored at 4℃ with moisture and cold stratification storage, the germination rates resulted in 78%, 76%, respectively. However, only 41% of the seeds germinated when they were stored at room temperature with moisture. No seed germination occurred when the seeds were stored either dry room temperature or at 4℃ without providing any moisture up to 10 weeks after sowing. The effect of temperature on seed germination was also analyzed. When the seeds were cultured at 20℃ until 5 weeks, 78% of them was germinated. However, as the temperature got either lower or higher than 20℃, the germination rate decreased. When the seeds were cultured at the temperatures 25℃, 15℃, 10℃ and 5℃ until 5 weeks, the germination rates were 64%, 69%, 60% and 52%, respectively. The seed collection time also appeared to influence the germination rate. When the seeds were collected on October 10 and 20, the germination rates were 78 and 89%, respectively. However, the germination rate of seeds collected earlier than that period decreased dramatically.

대립 다수성 복분자딸기 선발에 관한 연구

김세현(Sea Hyun Kim),김만조(Mahn Jo Kim),이울(Uk Lee) 한국산림과학회 2002 한국산림과학회지 Vol.91 No.1

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