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전북지역 송아지 설사 유래 병원성 대장균의 병원성 인자 및 다제 내성 패턴
곽길한 ( Kil-han Kwak ),김선민 ( Seon-min Kim ),유영주 ( Yeong-ju Yu ),유정희 ( Jeong-hee Yu ),임미나 ( Mi-na Lim ),장유정 ( Yu-jeong Jang ),허진 ( Jin Hur ) 한국동물위생학회 2021 韓國家畜衛生學會誌 Vol.44 No.4
Pathogenic Escherichia coli (E. coli) is one among the most important agents of diarrhea in calves. From January to December 2021, 108 isolates from feces of calves with diarrhea were investigated for enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), shiga toxin-producing E. coli (STEC), enteroaggregative E. coli (EAEC), and enteroinvasive E. coli (EIEC) using real-time PCR. In addition, the genes for F5, F17 and F41 fimbriae were detected by PCR. The most frequently isolated pathotypes were EPEC/STEC (29 isolates), and ETEC/EPEC/STEC (29 isolates). ETEC/EPEC, and ETEC/STEC were also found in 10 isolates. EPEC, STEC, and ETEC were detected in 13, 11, and 6 respectively. EAEC, and EIEC was not detected. Antimicrobial resistance test was carried out by agar disc diffusion method with 14 antimicrobials. Among 108 pathogenic E. coli isolates, 107 isolates were resistant to at least one of 14 antibiotics used in this study, 99 (91.7%) were resistant to two or more antimicrobials, and a single remarkable isolate was resistant to 14 antimicrobials. The isolates were primarily resistant to penicillins, streptomycin, tetracycline, ceftiofur, Trimethoprim/sulfamethoxazole, Kanamycin, and Ciprofloxacin. The high rate of resistance in pathogenic E. coli, sometimes to multiple drugs, may complicate future options for treating human infections. These results may bu used for diagnosis and therpeitic purposes in calves with diarrhea.
국내 사육 꿩에서 분리된 뉴켓슬병 바이러스의 hemagglutinin-neuraminidase(HN) 유전자의 클론닝과 염기서열 분석
장경수 ( Kyung Soo Chang ),곽길한 ( Kil Han Kwak ),장승익 ( Seung Ik Jang ),김지영 ( Ji Young Kim ),김태용 ( Tae Yong Kim ),송영환 ( Young Hwan Song ),송희종 ( Hee Jong Song ),전무형 ( Moo Hyung Jun ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.3
The gene encoding the HN protein from the CBP-1 strain, a heat stable Newcastle disease virus (NDV) isolated from diseased pheasants in Korea, was characterized by reverse transcriptasepolymerase chain reaction(RT-PCR) and the nucleotide and amino acid sequences were analyzed following cloning of the HN gene. In all of the NDV strains studied, a 1.75 kb size cDNA fragment for the HN gene was generated by RT-PCR and smaller specific band sizes harboring the internal portions of the HN gene were also detected by using four pars of primers. The RT-PCR was sensitive enough to detect viral transcripts when the virus titer was above 25 hemagglutination units. The amplified 1.75 kb cDNA was cloned into a BcamHI site of the pVL1393 Baculo transfer vector. The nucleotide sequences of the 1,758 bp HN gene from the CBP-1 strain were determined by the dye terminator cyclic sequencing method. The gene sequences were compared among the strains of CBP-1, Texas GB, Beaudette C, LaSota, Bl and Ulster. The homology of the CBP-1 HN gene to other HN variants was 97.8% to Texas GB, 98.4% to Beaudette C, 95.4% to LaSota, 95.6% to 131 and 90.2% to Ulster. As the deduced 577 amino acid sequences were compared among the strains, the homology for CBP-1 HN appeared to be 96.7% to Texas GB, 97.9% to Beaudette C, 95.5% to LaSota, 95.5% to BI and 92.7% to Ulster. It was evident that the amino acid sequences included 5 sites for N-asparagine linked glycosylation and 12 cysteine residues. The three conserved leucine residues within the predicted transmembrane domain of the HN protein are amino acid 30, 37 and 44. The three antigenic sites on the HN protein of NDV are amino acids 347 (Glu), 481 (Asn) and 495 (Glu). These data indicate that the genotype of the CBP-1 strain is more closely associated with the strains of Texas GB and Beaudette C than it is for the LaSota, BI and Ulster strains.
전북 익산지역 종계 및 육계에서 전염성빈혈 항체가 조사
엄성심 ( Sung Shim Um ),곽길한 ( Kil Han Kwak ),이정원 ( Joung Won Lee ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.2
From January to November in 2010, a result of chicken infectious anemia virus (CIAV) antibodies ELISA test about 500 number in 25 broiler breeder farms and 500 number in a broiler chickens slaughterhouse from 25 farms in Iksan area, Jeonbuk. The result of 100% (25/25) positive rate in broiler breeder farms group and 98.6% (493/500) positive rate from each individual. In each week-age group, 98.8% of 18~20 weeks and 98.3% of 21~24 weeks showed positive without any significant differences. In slaughterhouse case, broilers from the farms showed 36.0% (9/25) positive rate and each individual showed 20.4% (102/500) positive rate.
Brittany Spaniel에서 Proteus mirabilis에 의한 유방염 발생: 증례
이정원 ( Jeong Won Lee ),추금숙 ( Keum Suk Chu ),곽길한 ( Kil Han Kwak ),고원석 ( Won Seuk Ko ),송희종 ( Hee Jong Song ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.3
The dog breeding kennel bacterial infection are very significant in perinatal mortality. In many case, Staphylococcus aureus, Streptococcus, and Escherichia coli were infected in intra-uterine or by the genital tract to the puppies, and they are cause of septicemic death of the puppies and clinical mastitis of bitch, leading to septicemic death of newborn puppies. Severe mastitis due to bacterial infection was diagnosed in a 2 year-old female Brittany Spaniel which loss 6 puppies with odor small rice sharp white diarrhea of nine puppies. Bright curd milk and intestinal sample were inoculated on MacConkey agar, blood agar and brain heart infusion agar, and incubated at 37°C for 24-48 hrs. Gram negative colonies isolated from these sample which were characterized as Glu, Ure, H2S, Orn, Cit, and Cl, and were identified by Microscan Walk- Aways Baxter, American Type Culture Collection, USA) as Proteus mirabils. The isolate was more sensitive to ampicillin, gentamicin, cefoxitin, cefuroxime, and cefazidime. In this results, we confirmed that cause bacteria of septicemic death in puppies was P. mirabilis.
정재명 ( Jae Myong Jeong ),조성우 ( Sung Woo Jo ),곽길한 ( Kil Han Kwak ),서석열 ( Seok Yull Seo ) 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.2
It`s a case of the goat farm reared about 60 heads in Imsil county was outbreak Moniezia expensa infection. And 18 heads of less than 6 month olds goat were died. During the 2005 summer, morbidity and accumulative mortality were more than 60% and 30%, respectively less than 6 month young goat. Most young goat were suffer from diarrhea, severe weight losses, emaciations, and died. In necropsy, there were about 0.7-2.5m of 2-8 adult M expensa in the small intestinal lumen, swollen mesenteric lymph nodes. Slight hemorrhages were seen in lung and moderate hemorrhages were seen in mucous membrane of small intestine. Also various species(Trichostrongylus spp, Haemo-nchus spp, Eimeria spp) of parasite eggs were seen in fecal test. Pasteurella hemolytica was identified in lung by the API kit(Biomerieux Co. Ltd) for biochemical test. The minimal inhibitory concentration(MIC) results, trimethoprim/sulfamethoxazole, erythromycin, tyrosine, gentamycin, enrofloxacin, and norflocxacin were selected sensitive antibiotics.
Clostridium perfringens 감염에 의한 한우 집단 폐사 증례
이정원 ( Joung Won Lee ),김지영 ( Ji Young Kim ),곽길한 ( Kil Han Kwak ),육현수 ( Hyun Su Yuk ),손구례 ( Ku Rye Shon ),고원석 ( Won Seuk Koh ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.1
This is a case report that 24 heads of cattle suddenly died without clinical signs in a Hanwoo farm. The cause of death was Clostridium perfringens enterotoxemia resulted from them with feeding leftover food. The clinical signs were observed just before the death; increase of heart rate, shallow and rapid respiration, amyostasia, spasm and so forth. In autopsy, blood coagulation disorder, a little abdominal inflation, hepatomegaly and different size of red spots, congestion hemorrhage and undercurrent of bloody exudation were observed in the entire parts of small and large intestines. C. perfringens were isolated from the substantive organs, and a unique fragment of 405bp C. perfringens was amplified by PCR. Therefore, this case was diagnosed as enterotoxemia caused by α-toxin of C. perfringens A type.
전염성기관지염 및 뉴캣슬병 백신을 접종한 육계에서 ELISA 및 HI 항체가 비교
고원석 ( Won Seok Koh ),이정원 ( Jeoung Won Lee ),곽길한 ( Kil Han Kwak ),권정택 ( Jung Taek Kwon ),송희종 ( Hee Jong Song ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.1
To compare of serum antibody titers using ELISA and HI, serum samples were collected from 100 breeders and their progeny 550 broilers. The breeders and broilers were vaccinated with infectious bronchitis (IB)- and Newcastle disease(ND)-viruses according to general vaccination program. The antibodies in serum samples against IB and ND viruses were detected by enzyme-linked immunosorbent assay (ELISA) using commercial ELISA kit and hemagglutination inhibition(HI) test. Geometric mean titer (GMT) of ELISA and HI titers were monitored from 1-day-old to 35-day-old broilers and compared to those of breeder chickens. The antibody titers of breeders vaccinated with IB virus showed 47,800, ELISA and 7.2, HI, respectively. Progeny chicks, 1-day-old, vaccinated with IBV showed high antibody titers than those of breed chickens. Those chicks were maintained protective antibody levels until 11-day-old. From 14-day-old, the antibody level decreased below protective levels. In ND, breeders serum antibody titers ELISA and HI were 30,200 GMT and 8.7 HI titer, respectively. On 1-day-old chicks, antibody levels was decreased to half in ELISA(16,270) compared with those of breeders, but HI titers was 7.4. Progeny broilers, protective antibody level was maintained until 14- day-old by ELISA, but at 11-day-old by HI titers. After then, ND antibody titer was continuously decreased underdefense level. These result indicated that the ELISA method be more sensitive than HI titration to detect serum antibody level for IBV and NDV.