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전북지역 염소에서 Coxiella burnetii 감염실태 조사
강수진 ( Su-jin Gang ),정재명 ( Jae-myong Jeong ),김현관 ( Hyun-kwan Kim ),이재욱 ( Jae-wook Lee ),손구례 ( Ku-rye Shon ),박태욱 ( Tae-wook Park ) 한국가축위생학회 2016 韓國家畜衛生學會誌 Vol.39 No.4
The prevalence of Coxiella burnetii in native Korean goats in Jeonbuk province was investigated using ELISA (sera) and PCR (vaginal mucus). A total of 798 blood samples from 189 farms were collected in 2013 and 2015. Sero-prevalence was 13.6% in 2013 and 15.7% in 2015. Tracking survey of six seropositive farms on the prevalence of C. burnetii was conducted. 137 (41.4%) out of 331 goats were seropositive and 47 (17.6%) out of 267 goats were positive in PCR. The higher seropositivity observed in adult goats and female goats significantly, the seroprevalence of C. burnetii was higher as age increased (<1 y=13.2%∼≥5 y=100%)(P<0.05) and female goats (44.8%) was higher than male goats (24.1%) on the seroprevalence (P<0.05). 21 (7.9%) goats positive in PCR was seronegative. The prevalence of C. burnetii in native Korean goats in slaughter house was 30.0% in ELISA and 11.5% in PCR. Significantly, female goats (62.8%) was higher than male goats (12.3%) on the seroprevalence (P<0.05). Based on these data, C. burnetii was easily exposed in domestic animals: native Korean goats-related areas such as farms and slaughter house.
전북지역 돼지유행성설사 바이러스 Spike 유전자 염기서열 및 계통분석
박미연 ( Mi-yeon Park ),문보미 ( Bo-mi Moon ),강수진 ( Su-jin Gang ),이종하 ( Jong-ha Lee ),박진우 ( Jin-woo Park ),조성우 ( Sung-woo Cho ),허철호 ( Cheol-ho Her ) 한국동물위생학회(구 한국가축위생학회) 2021 韓國家畜衛生學會誌 Vol.44 No.2
Although many swine farms continuously vaccinated to sow to prevent Porcine epidemic diarrhea(PED), PED has occurred annually in swine herds in Jeonbuk province, Korea. In the present study, the small intestine and feces samples from 17 farms where severe watery diarrhea and death of newborn piglets occurred in 2019 were collected, amplified by RT-PCR and determined the complete nucleotide sequences of the spike (S) glycoprotein genes of nine Jeonbuk PEDV isolates. The spike (S) glycoprotein is an important determinant for molecular characterization and genetic relationship of PEDV. These nine complete S gene isolates were compared with other PEDV reference strains to identify the molecular diversity, phylogenetic relationships and antigenicity analysis. 9 field strains share 98.5∼100% homologies with each other at the nucleotide sequence level and 97.3∼100% homologies with each other at the amino acid level. The nine Jeonbuk PEDV isolates were classified into G2b group including a genetic specific signal, S-indels (insertion and deletion of S gene). In addition, comparisons the neutralizing epitopes of S gene between 9 field strains and domestic vaccine strains of Korea mutated 12-15 amino acids with SM-98-1 (G1a group) and mutated 0-3 amino acids with QIAP1401 (G2b group). Therefore, the development of G2b-based live vaccines will have to be expedited to ensure effective prevention of endemic PED in Korea. In addition, we will need to be prepared with periodic updates of preventive vaccines based on the PEDV variants for the re-emergence of a virulent strain.