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RISS 인기검색어
- 검색키워드
- 저자 : (Kunio Suetsuna) (검색결과 2 건)
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Suetsuna, Kunio,Shin, Suk U 한국수산학회 1997 한국수산과학회지 Vol.30 No.6
Docosahexaenoic acid(DHA) rich oil was obtained from blue fin tuna(Thunnus thynnus orientalis) orbital tissue with centrifugation of 12,000 rpm under vaccum(10^(-1) Torr) at 4℃, The effect of DHA rich oil(DHA content; 27.8%) on CCl₄-induced acute injury was investigated biochemically and histopathologically. Dosage of DHA rich oil on 24h before CCl₄-administration prevented significantly the increase of glutamic oxaloacetic transaminase(GOT) and glutamic pyruvic transaminase(GTP) values. No necrosis of hepatocytes was observed in rat livers treated with DHA oil on 24h prior to CCl₄-administration. These results suggested that DHA oil controls the accumulation of fat in the liver and prevented the liver injury.
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고정화 효소를 이용한 명태고기풀 수세액으로부터 Peptide 생산에 관한 연구
신석우(Suk U Shin),(Kunio Suetsuna) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.3
Peptides separated from fish paste washing liquid of an Alaska pollak(Theragria chalcogramma) were purified and characterized. The fish paste washing liquid (supernatant) was separated by centrifugation of fish paste homogenate. The fish paste washing liquid of 0.5% concentration was hydrolyzed for 24 hour at 50℃ by immobilized protease in bioreactor and decomposing liquid of protein having 50% decomposing rate (OPA method) was obtained. The crude peptide fractions were obtained from this liquid by Dowex 50w(H^+) column chromatography. Purified peptides(SP-fraction peptides) were fractionated by using SP-Sepadex C-25(H^+) column chromatography. Molecular weights and amino acid compositions of these peptides were estimated by Sephadex G-50 column chromatography and HPLC, respectively. When the washed peptides was eluated with 0.6-0.9% and 1.2-2.0% of NaCl, peptides composed of weakly basic amino acids and strongly basic amino acid were respectively eluted. Molecular weights of each peptide fractions showed the broad distribution from 1,000 Da to 3,000 Da in the order of SP-4>SP-3>SP-2>SP-1. Peptides contained a large quantity of glycine, arginine, glutamic acid, and alanine in the washed peptide and its SP-fractions, respectively.
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