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- 저자 : ( Xingguo Liang ) (검색결과 3 건)
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Taocun Dou,Manman Shen,Meng Ma,Liang Qu,Yongfeng Li,Yuping Hu,Jian Lu,Jun Guo,Xingguo Wang,Kehua Wang 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.3
Objective: Internal organs indirectly affect economic performance and well-being of animals. Study of internal organs during later layer period will allow full utilization of layer hens. Hence, we conducted a genome-wide association study (GWAS) to identify potential quantitative trait loci or genes that potentially contribute to internal organ weight. Methods: A total of 1,512 chickens originating from White Leghorn and Dongxiang Blue-Shelled chickens were genotyped using high-density Affymetrix 600 K single nucleotide polymorphism (SNP) array. We conducted a GWAS, linkage disequilibrium analysis, and heritability estimated based on SNP information by using GEMMA, Haploview and GCTA software. Results: Our results displayed that internal organ weights show moderate to high (0.283 to 0.640) heritability. Variance partitioned across chromosomes and chromosome lengths had a linear relationship for liver weight and gizzard weight (R2 = 0.493, 0.753). A total of 23 highly significant SNPs that associated with all internal organ weights were mainly located on Gallus gallus autosome (GGA) 1 and GGA4. Six SNPs on GGA2 affected heart weight. After the final analysis, five top SNPs were in or near genes 5-Hydroxytryptamine receptor 2A, general transcription factor IIF polypeptide 2, WD repeat and FYVE domain containing 2, non-SMC condensin I complex subunit G, and sonic hedgehog, which were considered as candidate genes having a pervasive role in internal organ weights. Conclusion: Our findings provide an understanding of the underlying genetic architecture of internal organs and are beneficial in the selection of chickens.
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( Xiaoming Pan ),( Yanfang Zhang ),( Xuejiao Sha ),( Jing Wang ),( Jing Li ),( Ping Dong ),( Xingguo Liang ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3
White spot syndrome virus (WSSV) is a major threat to the shrimp farming industry and so far there is no effective therapy for it, and thus early diagnostic of WSSV is of great importance. However, at the early stage of infection, the extremely low-abundance of WSSV DNA challenges the detection sensitivity and accuracy of PCR. To effectively detect low-abundance WSSV, here we developed a pre-amplification PCR (pre-amp PCR) method to amplify trace amounts of WSSV DNA from massive background genomic DNA. Combining with normal specific PCR, 10 copies of target WSSV genes were detected from ~10<sup>10</sup> magnitude of backgrounds. In particular, multiple target genes were able to be balanced amplified with similar efficiency due to the usage of the universal primer. The efficiency of the pre-amp PCR was validated by nested-PCR and quantitative PCR, and pre-amp PCR showed higher efficiency than nested-PCR when multiple targets were detected. The developed method is particularly suitable for the super early diagnosis of WSSV, and has potential to be applied in other low-abundance sample detection cases.
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Finite-time Asymmetric Bipartite Consensus for Signed Networks of Dynamic Agents
Xing Guo,Jinling Liang,Jianquan Lu 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.4
This paper addresses the finite-time asymmetric bipartite consensus problem for multi-agent systems(MAS) associated with signed graphs. Different from bipartite consensus in the previous literatures, asymmetricbipartite consensus means that the states of all agents will converge to two values with different signs and modulus. Two distinct nonlinear consensus control protocols are constructed for the considered system to achieve the finitetimeasymmetric bipartite consensus and the fixed-time asymmetric bipartite consensus, respectively. Under thefirst proposed protocol, it is shown that within a finite time, the considered system can realize asymmetric bipartiteconsensus. To strengthen the obtained result, the second protocol is proposed, which guarantees that all agentscould achieve the asymmetric bipartite consensus in fixed time, that is, the finite-time consensus can be reachedbefore a settling time which is irrelevant with the initial states of the agents. Finally, numerical simulations aregiven to verify effectiveness of the proposed consensus control protocols.
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