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Right lumbar lymph trunk injury after right laparoscopic donor nephrectomy: a case report
Le Thanh Dung,Le Nguyen Vu,Than Van Sy,Tran Ha Phuong,Ninh Viet Khai,Dao Xuan Hai,Nguyen Quang Nghia 대한이식학회 2023 Korean Journal of Transplantation Vol.37 No.2
Laparoscopic donor nephrectomy (LDN) is increasingly popular because of its advantages over open surgery. Chyle leak after donor nephrectomy is a rare but potentially lethal complication if not treated appropriately. We describe a case of a 43-year-old female patient with no remarkable history who presented a chyle leak on day 2 after right transperitoneal LDN. Since conservative treatment failed, the patient underwent magnetic resonance imaging (MRI) and intranodal lipiodol lymphangiography, which confirmed the chyle leak from the right lumbar lymph trunk into the right renal fossa. The chyle leak was percutaneously embolized twice, on postoperative day (POD) 5 and POD 10, by a mixture of N-butyl-2-cyanoacrylate and lipiodol. The drainage fluid decreased significantly after the second embolization. The subhepatic drainage tube was withdrawn on POD 14, and the patient was discharged on POD 17. MRI lymphangiography and intranodal lipiodol lymphangiography effectively identified the chyle leak point. Percutaneous embolization seems to be a safe, effective method for treating high-output chyle leaks.
Burned Area Detection After Wildfire Using Landsat 7 ETM+ SLC-off Images
Quoc, Khanh Le,Sy, Tan Nguyen,Nhat, Thanh Nguyen Thi,Thanh, Ha Le The Institute of Electronics and Information Engin 2013 IEIE Transactions on Smart Processing & Computing Vol.2 No.3
The increasing demand for monitoring wildfires and their impact on the land surface have prompted studies of burned area extraction and analysis. To differentiate burned and unburned area, the earlier method of the Moderate Resolution Imaging Spectro-radiometer (MODIS) Burned Area Detection Algorithm was proposed to estimate the change in land surface based on the reflectance energy. The energy, whose wavelengths are sensitive to burning, was selected to calculate the change parameter $Z_{score}$. This method was applied using the MODIS images to produce a MODIS Burned Area product. The approach was to simplify this algorithm to make it compatible with the Landsat 7 ETM+ SLC-off images. To extract the refined version of burned regions, post-processing was carried out by applying a median filter, dilation morphology algorithm, and finally a gap filling method. The experimental results showed that the detailed burned areas extracted from the proposed method exhibited more spatial details than those of the MODIS Burned products in the large U.S areas. The results also revealed the discontinuous distribution of burned regions in Vietnam forests.
Mortality following transarterial embolization due to hemorrhage after liver venous deprivation
Thanh Dung Le,Van Sy Than,Minh Duc Nguyen,Hoai Linh Vu,Xuan Hai Dao,Hong Son Trinh 소화기인터벤션의학회 2022 Gastrointestinal Intervention Vol.11 No.2
Liver venous deprivation (LVD) is considered to be a safe and effective method that induces more rapid and important liver hypertrophy before major hepatectomy in comparison with portal vein embolization (PVE) alone. Bleeding complications after LVD or PVE are rare, but can be a life-threatening event. Herein, we report a case of perihepatic and extended subcapsular hematoma of the liver after LVD. Transarterial embolization was performed using gelfoam to control the hemorrhage; however, the patient developed irreversible liver failure and passed away after 38 days of treatment. In patients with LVD or PVE, severe post-procedural bleeding poses a substantial challenge for treatment. Arterial embolization should be carefully considered to avoid liver failure and even death, regardless of whether temporary embolization is used.
Thanh Dung Le,Minh Duc Nguyen,Van Sy Than 소화기인터벤션의학회 2022 International journal of gastrointestinal interven Vol.11 No.1
The liver is the most frequently injured organ during abdominal trauma. Vascular injuries account for approximately 3% to 25% of all cases of liver trauma and are typically treated by transarterial embolization (TAE). We describe a case of American Association for the Surgery of Trauma grade V liver injury with a very large pseudoaneurysm and an arteriohepatic venous fistula (AHVF), which failed to respond to TAE. This case was successfully treated using a direct transhepatic glue injection without complications. AHVF is an exceedingly rare type of vascular injury in blunt trauma. Direct transhepatic glue injections could represent a reliable alternative to TAE for the treatment of visceral pseudoaneurysms, especially if TAE fails to eliminate the lesion.
Mortality following transarterial embolization due to hemorrhage after liver venous deprivation
Thanh Dung Le,Van Sy Than,Minh Duc Nguyen,Hoai Linh Vu,Xuan Hai Dao,Hong Son Trinh 소화기인터벤션의학회 2022 International journal of gastrointestinal interven Vol.11 No.2
Liver venous deprivation (LVD) is considered to be a safe and effective method that induces more rapid and important liver hypertrophy before major hepatectomy in comparison with portal vein embolization (PVE) alone. Bleeding complications after LVD or PVE are rare, but can be a life-threatening event. Herein, we report a case of perihepatic and extended subcapsular hematoma of the liver after LVD. Transarterial embolization was performed using gelfoam to control the hemorrhage; however, the patient developed irreversible liver failure and passed away after 38 days of treatment. In patients with LVD or PVE, severe post-procedural bleeding poses a substantial challenge for treatment. Arterial embolization should be carefully considered to avoid liver failure and even death, regardless of whether temporary embolization is used.
Thanh Dung Le,Minh Duc Nguyen,Van Sy Than 소화기인터벤션의학회 2022 Gastrointestinal Intervention Vol.11 No.1
The liver is the most frequently injured organ during abdominal trauma. Vascular injuries account for approximately 3% to 25% of all cases of liver trauma and are typically treated by transarterial embolization (TAE). We describe a case of American Association for the Surgery of Trauma grade V liver injury with a very large pseudoaneurysm and an arteriohepatic venous fistula (AHVF), which failed to respond to TAE. This case was successfully treated using a direct transhepatic glue injection without complications. AHVF is an exceedingly rare type of vascular injury in blunt trauma. Direct transhepatic glue injections could represent a reliable alternative to TAE for the treatment of visceral pseudoaneurysms, especially if TAE fails to eliminate the lesion.
( Vu Thi Thu Hang ),( Dinh Thi Quyen ),( Thi Tuyet Dao ),( Sy Le Thanh Nguyen ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.3
A novel gene coding for an endo-β-1,4-mannanase (manA) from Bacillus subtilis strain G1 was cloned and overexpressed in P. pastoris GS115, and the enzyme was purified and characterized. The manA gene consisted of an open reading frame of 1,092 nucleotides, encoding a 364-aa protein, with a predicted molecular mass of 41 kDa. The β-mannanase showed an identity of 90.2-92.9% (≤95%) with the corresponding amino acid sequences from B. subtilis strains deposited in GenBank. The purified β- mannanase was a monomeric protein on SDS-PAGE with a specific activity of 2,718 U/mg and identified by MALDITOF mass spectrometry. The recombinant β-mannanase had an optimum temperature of 45oC and optimum pH of 6.5. The enzyme was stable at temperatures up to 50oC (for 8 h) and in the pH range of 5-9. EDTA and most tested metal ions showed a slightly to an obviously inhibitory effect on enzyme activity, whereas metal ions (Hg2+, Pb2+, and Co2+) substantially inhibited the recombinant β-mannanase. The chemical additives including detergents (Triton X- 100, Tween 20, and SDS) and organic solvents (methanol, ethanol, n-butanol, and acetone) decreased the enzyme activity, and especially no enzyme activity was observed by addition of SDS at the concentrations of 0.25-1.0% (w/v) or n-butanol at the concentrations of 20-30% (v/v). These results suggested that the β-mannanase expressed in P. pastoris could potentially be used as an additive in the feed for monogastric animals.