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( Nasronudin ),( Bimo Aksono ),( Bimo D Lukito ),( Brian E Rachman ),( Noordiansyah ),( Retno Indrawati ),( Retno P Rahayu ),( M Inge Lusida ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
NASRONUDIN1, Bimo AKSONO1, Bimo D LUKITO4, Brian E RACHMAN2, NOORDIANSYAH7, Retno INDRAWATI1, Retno P RAHAYU1, M Inge LUSIDA1 Institute of Tropical Disease, Airlangga University, Indonesia1, Medical Faculty Airlangga University - Dr Soetomo Hospital, Indonesia2, Medical Faculty Airlangga University - Dr Soetomo Hospital, Indonesia3, Medical Faculty Airlangga University - Dr Soetomo Hospital, Indonesia4, Faculty of Dentistry, Airlangga University, Indonesia5, Faculty of Dentistry, Airlangga University, Indonesia6, Airlangga University Hospital, Indonesia7 Background: WNV is a mosquito-borne zoonotic arbovirus, belongs to the genus Flavivirus in the family Flaviviridae. Although WNV infection in human has been reported for decades in several parts of the world, but it was diagnosed recently in 2014 in Indonesia. Approximately 80% of WNV infections in humans do not develop any symptoms. Elderly or those with immunosuppression, such as HIV/AIDS, are at greater risk for serious illness. Phylogenetic analysis has divided WNV into mainly Lineage 1(L1) and Lineage 2 (L2), which is geographically specifi c. Aims: To determine and phylogenetic analyse of WNV on HIV/AIDS Patients. Methods: The subjects were HIV/AIDS patients in Airlangga University Hospital. Examination of blood samples were performed in Institute of Tropical Disease Airlangga University. This study has been approved by Ethical Committee. A total of 30 HIV/ AIDS patients were enroled after obtainined informed consent, and examined for WNV RNA using RT-PCR in the envelope region (408 bps). Results: The results showed that 8 (25.81%) of 30 HIV/AIDS patients were WNV positive. The phylogenetic of one WNV strain in this study showed that it belongs to the L2. Only recently were those strains of L2 identifi ed outside of Africa. Conclusions: We found WNV as one of the potential coinfections in HIV/AIDS patients in Indonesia, and therefore it may in other countries too. Phylogenetic analysis of one strain revealed that the virus clustered in the lineage 2.
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( Brian Eka Rachman ),( Retno P Rahayu ),( Nasronudin Nasronudin ),( Noordiansyah Noordiansyah ),( Bimo D Lukito ),( Retno Indrawati ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: The HIV infection is associated with immunecompromised and rising in opportunistic infection. Therefore, it is predicted that death caused by HIV/AIDS would increase. The main problem for HIV/AIDS patients are opportunistic infection caused by Japanese Encephalitis (JEV) which is rarely found and diffi cult to diagnosed in Indonesia nowadays. Methods: This study is an observational descriptive. Cross sectional technique was used in this study. The amount of samples were 30 HIV/AIDS patients. Enzyme-linked Immunosorbent Assay (ELISA) was used to test IgG and IgM, and Real Time Polimerase chain reaction (RT-PCR). The sample was obtained from HIV/AIDS patients` blood, then CD4 and viral load were tested to detect JEV secondary infection. Results: There were 93,54% of HIV/AIDS patients have undergone ARV therapy for 1-3 years. HIV/AIDS is mostly sexually transmitted with 67,74%. The distribution of HIV/AIDS patients in stage I is 41,94%, 35,48% in stage II, and 22,58% in stage III. The HIV>4x102 viral load test revealed 20% of the samples are infected by JEV. The intention of MDGs (Millenium Development Goals) is to achieve no new infection, no discrimination, no death associated to AIDS are expected to contribute in order to decrease death through early detection of secondary infection. This study might be used as new policies in impeding HIV/AIDS, so the MDGs target could be reached. Conclusions: This research shows that ARV therapy is not enough to prevent secondary infection, such as JEV in HIV/AIDS patients. Therefore, it is necessary to give antimicrobes therapy and adequate nutrional supports.
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