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( Thi Thuong Thuong Nguyen ),( Tham Thi Duong ),( Hyang Burm Lee ) 한국균학회 2016 Mycobiology Vol.44 No.4
While surveying the diversity of fungi of the order Mucorales, two isolates, EML-PUKI12-1 and EML-PUKI06-1, were obtained from the gut of soldier fly larvae inhabiting the bulrush at a pond located in the Chonnam National University Arboretum, Gwangju, Korea. The isolates were confirmed as Mucor irregularis and Mucor fragilis species, respectively, based on the morphological characteristics and phylogenetic analysis of rDNA internal transcribed spacer region. Such mucoralean species belonging to undiscovered taxa has not previously been described in Korea.
( Thi Thuong ),( Thuong Nguyen ),( Hyang Burm Lee ) 한국균학회 2016 韓國菌學會誌 Vol.44 No.4
During a survey of fungal diversity of the order Mortierellales, a zygomycete strain, EML-YS717-1, was isolated from a freshwater sample collected from the Yeongsan River in Gwangju, Korea. Based on its morphological characteristics and a phylogenetic analysis of the internal transcribed spacer (ITS1 and ITS2) and 5.8S rDNA sequences, the strain was identified as Mortierella minutissima. To the best of our knowledge, M. minutissima, has not previously been authentically reported in Korea.
Map-based cloning to identify gene involving in male gametophyte development in Arabidopsis
Thi Hoai Thuong Nguyen,Hyo-Jin Park,Tien Dung Nguyen,Sung Aeong Oh,Soon Ki Park 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
In the course of map-based cloning, mutant genes are identified through linkage to specific region on genetic map. Here, we demonstrated gametophytic mutant line, named as AP-28-23, in which mutant gene was mapped on chromosome 2. Based on phenotypic analysis of mature pollen, mutant phenotype of AP-28-23 was classified into three classes, wild-type showing 2-4%, moderate 35-53% and severe type 97-100% on aberrant pollen frequencies, respectively. The severe type is completely sterilized with 100% unfertilized ovules. We also revealed that the transmission was reduced through male gametophyte in the AP-28-23 line. The transmission efficiency (TE) through the male gametophyte is only 0.67%, whereas in the female gametophyte is 89.87%.
An investigation of seasonal variations in the microbiota of milk, feces, bedding, and airborne dust
Nguyen Thuong Thi,Wu Haoming,Nishino Naoki 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.11
Objective: The microbiota of dairy cow milk varies with the season, and this accounts in part for the seasonal variation in mastitis-causing bacteria and milk spoilage. The microbiota of the cowshed may be the most important factor because the teats of a dairy cow contact bedding material when the cow is resting. The objectives of the present study were to determine whether the microbiota of the milk and the cowshed vary between seasons, and to elucidate the relationship between the microbiota. Methods: We used 16S rRNA gene amplicon sequencing to investigate the microbiota of milk, feces, bedding, and airborne dust collected at a dairy farm during summer and winter. Results: The seasonal differences in the milk yield and milk composition were marginal. The fecal microbiota was stable across the two seasons. Many bacterial taxa of the bedding and airborne dust microbiota exhibited distinctive seasonal variation. In the milk microbiota, the abundances of Staphylococcaceae, Bacillaceae, Streptococcaceae, Microbacteriaceae, and Micrococcaceae were affected by the seasons; however, only Micrococcaceae had the same seasonal variation pattern as the bedding and airborne dust microbiota. Nevertheless, canonical analysis of principle coordinates revealed a distinctive group comprising the milk, bedding, and airborne dust microbiota. Conclusion: Although the milk microbiota is related to the bedding and airborne dust microbiota, the relationship may not account for the seasonal variation in the milk microbiota. Some major bacterial families stably found in the bedding and airborne dust microbiota, e.g., Staphylococcaceae, Moraxellaceae, Ruminococcaceae, and Bacteroidaceae, may have greater influences than those that varied between seasons.
Study on time-dependent mechanical properties of polyurethane foams with various porosity
( Thi Thuong-thuong Nguyen ),곽보민,권영환 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.0
In this research, one-step bulk polymerization method is used to synthesize the PU matrix foam. The PU matrixes are prepared by the reaction of prepolymers, such as hydroxy terminated polybutadiene (HTPB), polycaprolactone triol (PCL) and ether modified dimethylsiloxane (PDMS), and isocyanate isophorone diisocyanate (IPDI) in the presence of different hollow particles (HGM H25, HOM 100MCA, mix of H25 and 100MCA) volume percentage ranging from 0 to 75 %. The viscoelastic behavior in flexible polyurethane composites has a relationship between the recoverability the foam’s shape and behavior after the composites were affected by compression or fatigue. To understand the viscoelastic behavior of the polyurethane foam, the relaxation stress, the compression set, the cyclic compression and the cyclic stress relaxation test are investigated.
Characterization of Two New Records of Zygomycete Species Belonging to Undiscovered Taxa in Korea
Nguyen, Thi Thuong Thuong,Lee, Seo Hee,Bae, Sarah,Jeon, Sun Jeong,Mun, Hye Yeon,Lee, Hyang Burm The Korean Society of Mycology 2016 Mycobiology Vol.44 No.1
During a biodiversity survey of undiscovered taxa in Korea, two zygomycetous fungal strains were isolated. The first strain, EML-FSDY6-1 was isolated from a soil sample collected at Dokdo Island in the East Sea of Korea in 2013, and the second strain, EML-DG-NH3-1 was isolated from a rat dung sample collected at Chonnam National University garden, Gwangju, Korea in 2014. Based on the morphological characteristics and phylogenetic analysis of the internal transcribed spacer, 18S and 28S rDNA, actin and translation elongation $factor-1{\alpha}$ genes. EML-FSDY6-1 and EML-DG-NH3-1 isolates were confirmed as zygomycete species, Absidia pseudocylindrospora and Absidia glauca, respectively. Neither species has previously been described in Korea.
( Thi Thuong Thuong Nguyen ),( Hyang Burm Lee ) 한국균학회 2016 韓國菌學會誌 Vol.44 No.4
During a survey of fungal diversity in insect guts in Korea, two fungal strains, EML-PGH2 and EML-PUKI88, were isolated from the gut of grasshopper and soldier fly larvae inhabiting the bulrush plants at a pond located in the Chonnam National University Arboretum, Gwangju, Korea. Based on their morphological characteristics and a phylogenetic analysis of the internal transcribed spacer (ITS1 and ITS2) and 5.8S rDNA sequences, the strains were identified as Blakeslea trispora. To our knowledge, the zygomycete species B. trispora has not been previously described in Korea.
Thuong, Phuong Thien,Hung, Tran Manh,Khoi, Nguyen Minh,Nhung, Hoang Thi My,Chinh, Nguyen Thi,Quy, Nguyen Thi,Jang, Tae Su,Na, MinKyun 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.3
Four lignans, meso-dihydroguaiaretic acid (DHGA), macelignan, fragransin $A_2$ and nectandrin B, were isolated from the seeds of Myristica fragrans (Vietnamese nutmeg) and investigated for their cytotoxic activity against eight cancer cell lines. Of these, DHGA exhibited potent cytotoxicity against H358 with $IC_{50}$ value of $10.1{\mu}M$. In addition, DHGA showed antitumor activity in allogeneic tumor-bearing mice model.
Thuong Nhan Phu Nguyen,Chi Khang Van,Thu Trang Thi Nguyen,Thuan Van Tran,Quang Binh Hoang,Long Giang Bach 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.13
This study aimed to evaluate the encapsulation of pomelo (Citrus grandis (L.) Osbeck) essential oils using the spray drying technique. The parameters of the process include concentration of maltodextrin (20–35% by wt%/wt%), concentration of essential oil (1–2.5% by wt%/wt%), inlet temperature of spray drying (120–180 °C), and feed flow rates (120–240 mL/h) were soundly examined. The utilization of suitable parameters as the concentration of maltodextrin at 30% (by wt%/wt%), the concentration of essential oil at 1.5% (by wt%/wt%), the inlet temperature of 140 °C, and feed flow rate of 120 mL/h showed the highest drying yields (90.05%), microencapsulation yield (75.59%), and microencapsulation efficiency (89.44%). TGA and DSC results verified higher stability of Citrus grandis essential oil after encapsulation. The encapsulation of pomelo essential oils maintained most of the major components in comparison with the non-encapsulated essential oils without any significant changing in powder-obtained quality.