Fabrication of PHBV/Keratin Composite Nanofibrous Mats for Biomedical Applications

Yuan, Jiang,Xing, Zhi-Cai,Park, Suk-Woo,Geng, Jia,Kang, Inn-Kyu,Yuan, Jiang,Shen, Jian,Meng, Wan,Shim, Kyoung-Jin,Han, In-Suk,Kim, Jung-Chul The Polymer Society of Korea 2009 Macromolecular Research Vol.17 No.11

Keratin is an important protein used in wound healing and tissue recovery. In this study, keratin was modified chemically with iodoacetic acid (IAA) to enhance its solubility in organic solvent. Poly(hydroxybutylate-co-hydroxyvalerate) (PHBV) and modified keratin were dissolved in hexafluoroisopropanol (HFIP) and electrospun to produce nanofibrous mats. The resulting mats were surface-characterized by ATR-FTIR, field-emission scanning electron microscopy (FE-SEM) and electron spectroscopy for chemical analysis (ESCA). The pure m-keratin mat was cross-linked with glutaraldehyde vapor to make it insoluble in water. The biodegradation test in vitro showed that the mats could be biodegraded by PHB depolymerase and trypsin aqueous solution. The results of the cell adhesion experiment showed that the NIH 3T3 cells adhered more to the PHBV/m-keratin nanofibrous mats than the PHBV film. The BrdU assay showed that the keratin and PHBV/m-keratin nanofibrous mats could accelerate the proliferation of fibroblast cells compared to the PHBV nanofibrous mats.

The Inhibitory Effect of Plant Water Extracts on Tyrosinase and Elastase

Jia Yuan,Inshik Park 한국식품영양학회 2013 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2013 No.06

Research on Thymopentin Loaded Oral N-Trimethyl Chitosan Nanoparticles

Yuan, Xiao-Jia,Zhang, Zhi-Rong,Song, Qing-Guo,He, Qin The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.9

Peptides, although high efficacy and specificity in their physiological function, usually have low therapeutical activities due to their poor bioavailability when administrated orally. Nanoparticles have been regarded as a useful vector for targeted drug delivery system because they can protect drug from being degraded quickly and pass the gastrointestinal barriers. Here we described a novel oral N-trimethyl chitosan nanoparticles formulation containing thymopentin (Tp5-TMC-NP). N-trimethyl chitosan (TMC) was synthesized and then used to prepare Tp5-TMC-NP by ionotropic gelation. A three-factor, five-level CCD (Central Composite Design) design was used in the optimization procedure, with HPLC as the analyzing method. The resulting Tp5-TMC-NP had a regular spherical surface and a narrow particle size range with a mean diameter of 110.6 nm. The average entrapment efficiency was 78.8%. The lyophilized Tp5-TMC-NP formulation was stable in $4^{\circ}C\;or\;-20^{\circ}C$ after storage of 3 months without obvious changes in morphology, particle size, pH and entrapment ratio. The results of the flow cytometer determination showed that the ratio of $CD4^+/CD8^+$ of Wistar female rat given Tp5-TMC-NP (ig) was 2.59 time that of the group given Tp5 (ig).

Good to Great : Build strong and vital teams

Jia Yuan,Runtian Jing 보안공학연구지원센터(IJUNESST) 2014 International Journal of u- and e- Service, Scienc Vol.7 No.1

Tribology Property of Benzoxazine–Bismaleimide Composites with Hyperbranched Polysilane-Grafted Multi-Walled Carbon Nanotubes

YUAN JIA,HONGXIA YAN,Song Li,Tianye Liu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.6

To reveal the wear mechanism of hyperbranched polysilane (HBPSi) grafted multi-walled carbon nanotubes (HBPSi–MWCNTs) modified benzoxazine–bismaleimide (BOZ–BMI) resin (HBPSi– MWCNTs/BOZ–BMI), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and thermogravimetric analysis (TGA) were employed. The results indicated that the suitable addition of HBPSi–MWCNTs could largely enhance the tribological properties of BOZ– BMI composites. The worn surface of the composites showed that the severe wear of the BOZ– BMI resin was converted from adhesive wear to abrasive wear with the addition of HBPSi– MWCNTs. The excellent tribological properties can be attributed to the improved interfacial adhesion between HBPSi–MWCNTs and the BOZ–BMI resin matrix. The TGA demonstrated that the composite with 0.8 wt.% HBPSi–MWCNTs exhibits better thermal resistance; thus, it can inhibit adhesive wear during the friction process. The XPS spectra and the surface energy showed that the HBPSi–MWCNTs could be exposed on the worn surface of the composite to improve the anti-wear capacity of the composites further.

Single-Source-Precursor Synthesis of Hafnium-Containing Ultrahigh-Temperature Ceramic Nanocomposites (UHTC-NCs)

Yuan, Jia,Hapis, Stefania,Breitzke, Hergen,Xu, Yeping,Fasel, Claudia,Kleebe, Hans-Joachim,Buntkowsky, Gerd,Riedel, Ralf,Ionescu, Emanuel American Chemical Society 2014 Inorganic chemistry Vol.53 No.19

<P>Amorphous SiHfBCN ceramics were prepared from a commercial polysilazane (HTT 1800, AZ-EM), which was modified upon reactions with Hf(NEt<SUB>2</SUB>)<SUB>4</SUB> and BH<SUB>3</SUB>·SMe<SUB>2</SUB>, and subsequently cross-linked and pyrolyzed. The prepared materials were investigated with respect to their chemical and phase composition, by means of spectroscopy techniques (Fourier transform infrared (FTIR), Raman, magic-angle spinning nuclear magnetic resonance (MAS NMR)), as well as X-ray diffraction (XRD) and transmission electron microscopy (TEM). Annealing experiments of the SiHfBCN samples in an inert gas atmosphere (Ar, N<SUB>2</SUB>) at temperatures in the range of 1300–1700 °C showed the conversion of the amorphous materials into nanostructured UHTC-NCs. Depending on the annealing atmosphere, HfC/HfB<SUB>2</SUB>/SiC (annealing in argon) and HfN/Si<SUB>3</SUB>N<SUB>4</SUB>/SiBCN (annealing in nitrogen) nanocomposites were obtained. The results emphasize that the conversion of the single-phase SiHfBCN into UHTC-NCs is thermodynamically controlled, thus allowing for a knowledge-based preparative path toward nanostructured ultrahigh-temperature stable materials with adjusted compositions.</P><P>Amorphous SiHfBCN ceramics were prepared from a suitable single-source precursor. Annealing experiments of the SiHfBCN samples in an inert gas atmosphere at temperatures of 1300−1700 °C led to their conversion nanostructured UHTC-NCs, such as HfC/HfB<SUB>2</SUB>/SiC nanocomposites (in argon) and HfN/Si<SUB>3</SUB>N<SUB>4</SUB>/SiBCN nanocomposites (in nitrogen), thus revealing a convenient preparative approach to nanostructured ultrahigh-temperature stable materials starting from a greatly flexible single-source precursor.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/inocaj/2014/inocaj.2014.53.issue-19/ic501512p/production/images/medium/ic-2014-01512p_0016.gif'></P>

Effect of Fluorescence Light on Phenolic Compounds and Antioxidant Activities of Soybeans (Glycine max L. Merrill) during Germination

Ming Yuan,Xuejing Jia,ChunBang Ding,Haiqiong Zeng,Lei Du,Shu Yuan,Zhongwei Zhang,Qi Wu,Chao Hu,Jing Liu 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.5

Effects of light on phenolic compounds and antioxidant activities during germination of soybean seeds were studied. Soybean seeds were germinated in the presence and absence of light. Chlorophylls and morphological characteristics of sprouts were evaluated daily. Antioxidant activities were determined using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and ferric reducing activity power methods. Morphological characteristics of light-grown sprouts were superior to dark-grown sprouts and the metabolism of phenolic compounds was regulated by light. Light treatment improved accumulation of phenolic compounds in soybean sprouts and increased antioxidant activities. The optimum harvest time of light-grown sprouts was on the seventh day when sprouts achieved the best nutraceutical value. Green sprouts can serve as a good vegetable source for the human diet.

Mitogen-Activated Protein Kinase Signal Transduction in Solid Tumors

Lei, Yuan-Yuan,Wang, Wei-Jia,Mei, Jin-Hong,Wang, Chun-Liang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Mitogen-activated protein kinase (MAPK) is an important signaling pathway in living beings in response to extracellular stimuli. There are 5 main subgroups manipulating by a set of sequential actions: ERK(ERK1/ERK2), c-Jun N(JNK/SAPK), p38 MAPK($p38{\alpha}$, $p38{\beta}$, $p38{\gamma}$ and $p38{\delta}$), and ERK3/ERK4/ERK5. When stimulated, factors of upstream or downstream change, and by interacting with each other, these groups have long been recognized to be related to multiple biologic processes such as cell proliferation, differentiation, death, migration, invasion and inflammation. However, once abnormally activated, cancer may occur. Several components of the MAPK network have already been proposed as targets in cancer therapy, such as p38, JNK, ERK, MEK, RAF, RAS, and DUSP1. Among them, alteration of the RAS-RAF-MEK-ERK-MAPK(RAS-MAPK) pathway has frequently been reported in human cancer as a result of abnormal activation of receptor tyrosine kinases or gain-of-function mutations in genes. The reported roles of MAPK signaling in apoptotic cell death are controversial, so that further in-depth investigations are needed to address these controversies. Based on an extensive analysis of published data, the goal of this review is to provide an overview on recent studies about the mechanism of MAP kinases, and how it generates certain tumors, as well as related treatments.

Exploration of correction method of color reduction in Dun Huang fresco

( Li Jia Yuan ) 한국동양예술학회 2021 동양예술 Vol.52 No.-

돈황은 우리나라의 유명한 유적지이며 돈황 벽화 또한 예술적 가치가 높다. 돈황 벽화 분석을통해 우리나라의 민족 예술과 당시의 종교적 지위를 알 수 있으며, 관련 역사학자에게는 더 나은 연구방향을 제공할 수 있다. 돈황 벽화는 소재 선정이 비교적 진보적일 뿐만 아니라 당시 세계 최고의 색채 배합 기술을 사용하여 색채가 밝고 독특한 정취를 띠고 있다. 다만 시대적 침전을 거치면서 각각 다른 설비 장비가 복제를 할 때 돈황 벽화의 색상은 색상 교정과 복원 문제가 더욱 발생하게 된다. 이 같은 문제의 주요 원인은 장비간의 표시방법과 돈황 벽화의 자체적 퇴색이 갖는 뚜렷한 관계이다. 이에 본 연구는 돈황 벽화 색상 복원 및 교정 방법을 모색하였다. This paper will discuss the exploration of the color restoration correction method of Dun Huang murals, and I will discuss the significance of the color restoration of Dun Huang murals at first. The color of Dun Huang murals is the peak in the history of Chinese murals. The image color is very exquisite and has high artistic value and historical value. In addition, I would elaborate on the significance of color restoration correction, and analyze the importance of addition color mixing, subtraction color rendering and spatial color management theory for Dun Huang murals. And in the analysis of image distortion, I would take digital camera, color printer as an example to discuss. In the method of color correction of Dun Huang murals, the color distortion caused by digital camera and printer often cause the color deviation. Therefore, it is of great practical significance to discuss this two as examples. In the region matching of the color restoration correction system, however, the basic principle of its neural network and the color restoration system of Dun Huang murals can ensure the active color of the entire Dun Huang murals.

Comparisons in phytochemical components and in vitro digestion properties of corresponding peels, flesh and seeds separated from two blueberry cultivars

Mei-Jia Li,Yuan-Yuan Deng,Li-Hua Pan,Shui-Zhong Luo,Zhizheng Jiang 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.1

Highbush blueberries (HB) and rabbiteye blueberries (RB) were separated into peels, flesh, and seeds to assess the compositions of nutriment, anthocyanins, soluble sugars and fatty acids, and the in vitro digesting abilities. Total phenolics contents (TPC) of 51–56 mg GAE/g DW were found in blueberry peels. Compared with HB peels, RB peels showed much higher TPC, but only contained 35 phenolics and lacked peonidin-3-O-rutinoside. Glucose, fructose, and sucrose were all present in HB and RB, but RB flesh had a higher acid-sugar ratio. Unsaturated fatty acid concentrations in HB and RB seeds were comparable (26.65 and 26.43 mg/g, respectively). However, HB seeds have 35 fatty acids, but RB seeds lacked cis-4,7,10,13,16,19-docosahexaenoic acid and cis-10-pentadecenoic acid. The in vitro digestion test showed that the whole fruit/peels/flesh of RB had a higher recovery and bioavailability index of phenolics and anthocyanins. Therefore, the reuse of blueberry pomace needs to be emphasized.