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Han Sol Kim,Hongliang Li,Hye Won Kim,Sung Eun Shin,Mi Seon Seo,Jin Ryeol An,Kwon-Soo Ha,Eun-Taek Han,Seok-Ho Hong,Il-Whan Choi,Grace Choi,Dae-sung Lee,Won Sun Park 대한생리학회-대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.13 No.1
We investigated the inhibitory effect of escitalopram, a selective serotonin reuptake inhibitor (SSRI), on voltage-dependent K<sup>+</sup> (Kv) channels in freshly separated from rabbit coronary arterial smooth muscle cells. The application of escitalopram rapidly inhibited vascular Kv channels. Kv currents were progressively inhibited by an increase in the concentrations of escitalopram, suggesting that escitalopram inhibited vascular Kv currents in a concentration-dependent manner. The IC<sub>50</sub> value and Hill coefficient for escitalopram-induced inhibition of Kv channels were 9.54±1.33 µM and 0.75±0.10, respectively. Addition of escitalopram did not alter the steady-state activation and inactivation curves, suggesting that the voltage sensors of the channels were not affected. Pretreatment with inhibitors of Kv1.5 and/or Kv2.1 did not affect the inhibitory action of escitalopram on vascular Kv channels. From these results, we concluded that escitalopram decreased the vascular Kv current in a concentration-dependent manner, independent of serotonin reuptake inhibition.
( Han Sol Seo ),( Sunmin Lee ),( Digar Singh ),( Min Kyung Park ),( Young-suk Kim ),( Hye Won Shin ),( Sun A Cho ),( Choong Hwan Lee ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.8
Production of good Koji primarily depends upon the selection of substrate materials and fermentative microflora, which together influence the characteristic flavor and aroma. Herein, we performed comparative metabolomic analyses of volatile organic compounds (VOCs) and primary metabolites for Koji samples fermented individually with Bacillus amyloliquefaciens and Aspergillus oryzae. The VOCs and primary metabolites were analyzed using headspace solid phase microextraction (HS-SPME) followed by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). In particular, alcohols, ketones, and furans were mainly detected in Bacillus-fermented Koji (Bacillus Koji, BK), potentially due to the increased levels of lipid oxidation. A cheesy and rancid flavor was characteristic of Bacillus Koji, which is attributable to high content of typical ‘off-flavor’ compounds. Furthermore, the umami taste engendered by 2-methoxyphenol, (E,E)-2,4-decadienal, and glutamic acid was primarily detected in Bacillus Koji. Alternatively, malty flavor compounds (2-methylpropanal, 2-methylbutanal, 3-methylbutanal) and sweet flavor compounds (monosaccharides and maltol) were relatively abundant in Aspergillus-fermented Koji (Aspergillus Koji, AK). Hence, we argue that the VOC profile of Koji is largely determined by the rational choice of inocula, which modifies the primary metabolomes in Koji substrates, potentially shaping its volatolome as well as the aroma characteristics.
Seo, Han Sol,Lee, Sunmin,Singh, Digar,Shin, Hye Won,Cho, Sun A,Lee, Choong Hwan Elsevier 2018 Food chemistry Vol.266 No.-
<P><B>Abstract</B></P> <P>Untargeted metabolomics unraveled the effects of varying substrates (soybean, wheat, and rice) and inocula (<I>Aspergillus oryzae</I> and <I>Bacillus amyloliquefaciens</I>) on metabolite compositions of <I>koji</I>, a starter ingredient in various Asian fermented foods. Multivariate analyses of the hyphenated mass spectrometry datasets for different <I>koji</I> extracts highlighted 61 significantly discriminant primary metabolites (sugars and sugar alcohols, organic acids, amino acids, fatty acids, nucleosides, phenolic acids, and vitamins) according to varying substrates and inocula combinations. However, 59 significantly discriminant secondary metabolites were evident for <I>koji</I>-types with varying substrates only, <I>viz.</I>, soybean (flavonoids, soyasaponins, and lysophospholipids), wheat (flavones and lysophospholipids), and rice (flavonoids, fatty acids derivatives, and lysophospholipids). Independently, the substrates influenced primary metabolite compositions in <I>koji</I> (soybean > wheat, rice). The inocula choice of <I>A. oryzae</I> engendered higher carbohydrates, organic acids, and lipid derivative levels commensurate with high <I>α</I>-amylase and <I>β</I>-glucosidase activities, while <I>B. amyloliquefaciens</I> affected higher amino acids levels, in respective <I>koji</I> types.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Metabolomics revealed optimal substrate and inocula combination for preparing <I>koji</I>. </LI> <LI> Primary metabolites in <I>koji</I> were affected by substrates and microbial inocula. </LI> <LI> Primary metabolites except amino acids were higher in soybean substrate. </LI> <LI> Secondary metabolites varied largely according to substrate types. </LI> <LI> Flavonoids and soyasaponins abundant in soybean were higher in soybean <I>koji</I>. </LI> </UL> </P>
Bae, Han-Sol,Yoon, Won-Joon,Cho, Young-Dan,Islam, Rabia,Shin, Hye-Rim,Kim, Bong-Soo,Lim, Jin-Muk,Seo, Min-Seok,Cho, Seo-Ae,Choi, Kang-Young,Baek, Seung-Hak,Kim, Hong-Gee,Woo, Kyung-Mi,Baek, Jeong-Hwa Wiley (John WileySons) 2017 Journal of Bone and Mineral Research Vol. No.
<P>Cleidocranial dysplasia (CCD) is an autosomal dominant skeletal disorder caused by mutations in RUNX2, coding a key transcription factor of early osteogenesis. CCD patients suffer from developmental defects in cranial bones. Despite numerous investigations and clinical approaches, no therapeutic strategy has been suggested to prevent CCD. Here, we show that fetal administration of Entinostat/MS-275, a class I histone deacetylase (HDAC)-specific inhibitor, partially prevents delayed closure of cranial sutures in Runx2(+/-) mice strain of C57BL/6J by two mechanisms: 1) posttranslational acetylation of Runx2 protein, which stabilized the protein and activated its transcriptional activity; and 2) epigenetic regulation of Runx2 and other bone marker genes. Moreover, we show that MS-275 stimulates osteoblast proliferation effectively both in vivo and in vitro, suggesting that delayed skeletal development in CCD is closely related to the decreased number of progenitor cells as well as the delayed osteogenic differentiation. These findings provide the potential benefits of the therapeutic strategy using MS-275 to prevent CCD. (c) 2017 American Society for Bone and Mineral Research.</P>
Kim, Han Sol,Li, Hongliang,Kim, Hye Won,Shin, Sung Eun,Seo, Mi Seon,An, Jin Ryeol,Ha, Kwon-Soo,Han, Eun-Taek,Hong, Seok-Ho,Choi, Il-Whan,Choi, Grace,Lee, Dae-sung,Park, Won Sun The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.4
We investigated the inhibitory effect of escitalopram, a selective serotonin reuptake inhibitor (SSRI), on voltage-dependent $K^+$ (Kv) channels in freshly separated from rabbit coronary arterial smooth muscle cells. The application of escitalopram rapidly inhibited vascular Kv channels. Kv currents were progressively inhibited by an increase in the concentrations of escitalopram, suggesting that escitalopram inhibited vascular Kv currents in a concentration-dependent manner. The $IC_{50}$ value and Hill coefficient for escitalopram-induced inhibition of Kv channels were $9.54{\pm}1.33{\mu}M$ and $0.75{\pm}0.10$, respectively. Addition of escitalopram did not alter the steady-state activation and inactivation curves, suggesting that the voltage sensors of the channels were not affected. Pretreatment with inhibitors of Kv1.5 and/or Kv2.1 did not affect the inhibitory action of escitalopram on vascular Kv channels. From these results, we concluded that escitalopram decreased the vascular Kv current in a concentration-dependent manner, independent of serotonin reuptake inhibition.
Screening of Genetic Polymorphisms of <em>CYP3A4</em> and <em>CYP3A5</em> Genes
Jin Sol Lee,Hyun Sub Cheong,Lyoung Hyo Kim,Ji On Kim,Doo Won Seo,Young Hoon Kim,Myeon Woo Chung,Soon Young Han,Hyoung Doo Shin 대한생리학회-대한약리학회 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.6
Given the <em>CYP3A4</em> and <em>CYP3A5</em>’s impact on the efficacy of drugs, the genetic backgrounds of individuals and populations are regarded as an important factor to be considered in the prescription of personalized medicine. However, genetic studies with Korean population are relatively scarce compared to those with other populations. In this study, we aimed to identify <em>CYP3A4</em>/5 polymorphisms and compare the genotype distributions among five ethnicities. To identify <em>CYP3A4</em>/5 SNPs, we first performed direct sequencing with 288 DNA samples which consisted of 96 Koreans, 48 European- Americans, 48 African-Americans, 48 Han Chinese, and 48 Japanese. The direct sequencing identified 15 novel SNPs, as well as 42 known polymorphisms. We defined the genotype distributions, and compared the allele frequencies among five ethnicities. The results showed that minor allele frequencies of Korean population were similar with those of the Japanese and Han Chinese populations, whereas there were distinct differences from European-Americans or African-Americans. Among the phar-macogenetic markers, frequencies of CYP3A4*1B (rs2740574) and <em>CYP3A5</em>*3C (rs776742) in Asian groups were different from those in other populations. In addition, minor allele frequency of CYP3A4*18 (rs28371759) was the highest in Korean population. Additional in silico analysis predicted that two novel non-synonymous SNPs in <em>CYP3A5</em> (+27256C>T, P389S and +31546T>G, I488S) could alter protein structure. The frequency distributions of the identified polymorphisms in the present study may contribute to the expansion of pharmacogenetic knowledge.
서한솔(Han Sol Seo),남수현(Su Hyeon Nam),신현철(Hyun Chul Shin),권오병(Ohbyung Kwon) 한국IT서비스학회 2016 한국IT서비스학회 학술대회 논문집 Vol.2016 No.춘계
철학 사상 연구에서 어떤 문헌을 분석하여 어떤 철학 사조에 해당하는지를 판별하는 것은 전문가가 문헌을 읽고 판단해야 하므로 매우 많은 시간과 비용이 소요된다. 더구나 일반인의 입장에서 문헌의 내용이 어떤 철학에 속하는지를 정확하게 판단하는 것은 매우 어려운 일이다. 분석해야 하는 문헌이 방대할수록 이러한 비효율성과 부정확성의 문제는 더욱 심각하다. 따라서 본 연구의 목적은 서적이나 인터넷 뉴스, 블로그 등 비정형자료의 형태로 존재하는 철학 관련 문헌을 자동 분 류하는데 데이터 마이닝 기법들이 유용한지를 검증하는 것이다. 또한 각 데이터 마이닝 기법들의 성능을 비교하고 앙상블 기법을 적용하여 자동 분류 정확도를 높이고자 한다. 앙상블 기법의 적용결과는 일반인과 전문가 집단을 대상으로 한 응답 결과와 비교하여 평가고자 한다.
Screening of Genetic Polymorphisms of CYP3A4 and CYP3A5 Genes
Lee, Jin Sol,Cheong, Hyun Sub,Kim, Lyoung Hyo,Kim, Ji On,Seo, Doo Won,Kim, Young Hoon,Chung, Myeon Woo,Han, Soon Young,Shin, Hyoung Doo The Korean Society of Pharmacology 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.6
Given the CYP3A4 and CYP3A5's impact on the efficacy of drugs, the genetic backgrounds of individuals and populations are regarded as an important factor to be considered in the prescription of personalized medicine. However, genetic studies with Korean population are relatively scarce compared to those with other populations. In this study, we aimed to identify CYP3A4/5 polymorphisms and compare the genotype distributions among five ethnicities. To identify CYP3A4/5 SNPs, we first performed direct sequencing with 288 DNA samples which consisted of 96 Koreans, 48 European-Americans, 48 African-Americans, 48 Han Chinese, and 48 Japanese. The direct sequencing identified 15 novel SNPs, as well as 42 known polymorphisms. We defined the genotype distributions, and compared the allele frequencies among five ethnicities. The results showed that minor allele frequencies of Korean population were similar with those of the Japanese and Han Chinese populations, whereas there were distinct differences from European-Americans or African-Americans. Among the pharmacogenetic markers, frequencies of $CYP3A4^*1B$ (rs2740574) and $CYP3A5^*3C$ (rs776742) in Asian groups were different from those in other populations. In addition, minor allele frequency of $CYP3A4^*18$ (rs28371759) was the highest in Korean population. Additional in silico analysis predicted that two novel non-synonymous SNPs in CYP3A5 (+27256C>T, P389S and +31546T>G, I488S) could alter protein structure. The frequency distributions of the identified polymorphisms in the present study may contribute to the expansion of pharmacogenetic knowledge.