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RISS 인기검색어
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- 저자 : ( Gary Bauchan ) (검색결과 5 건)
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Mark S. Roh,Gary R. Bauchan,Charles Murphy,James A. Bunce 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.6
Three different injection molded bioplastic (BP) pots, BP 29, BP 32, and BP 45 pots, containing processed feather fibers, were evaluated. Growth and flowering of Lilium hybrid ‘Triumphator’ and Begonia ‘Bonfire’ were evaluated in new and reused BP pots. Net photosynthesis was measured and macro- and micro-elements were analyzed in ‘Triumphator’ lily. Ultrastructure of epidermis cells of ‘Triumphator’ lily were analyzed using a low temperature-scanning electron microscope (LT-SEM). Surface changes, the weight, and macro- and micro-elements of the BP pots were also analyzed. When lilies were grown in BP 32 pots, root and shoot growth were inhibited, flowering was delayed and the net photosynthesis was negative, grana development, and starch granules were not observed as compared to those in BP 45 pots. Changes in the surface of BP 32 pots after forcing lilies (used once) showed a distinct space between feather fibers and other constituents which could be due to the chemical changes resulting from absorption of water. When BP pots were analyzed for pH and macro- and micro-elements, the pH and the concentration of ammonium nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, and iron were significantly higher in the new BP 32 than those of BP 29 and BP 45 pots. Based on the growth and development of lily and begonia and net photosynthesis and grana development of lily, and foliar analysis of begonia grown in three different BP pots, it is concluded that BP 45 is recommended to grow lily and begonia when proper levels of nutrition are maintained during culture. Poor root growth and development of ‘Triumphator’ lily grown in new BP 32 pots could be associated with leaching of toxic substances once the pots absorb water during the culture.
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Mark S. Roh,Gary R. Bauchan,Masud S. Huda 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.1
This study was conducted to evaluate the growth and flowering of Begonia boliviensis A. DC. ‘Bonfire’when grown in the growing medium mixed with pellets from biobased plastic resins containing chicken feather fibers, and analyze macro- and micro-elements in soil and leaf tissues during different developmental stages of begonia. Growth, flowering, and foliar analysis of plants grown in 10 cm pots containing 95 grams medium mixed with 6 grams each of pellet 29 (P 29), 32 (P 32), 37 (P 37), or 40 (P 40), 3 grams of Na (P NaS), or 9 grams of 45 (P 45) were similar to the control plants. Extra nitrogen was available from P 32 and P 37 based on the growing medium and foliar analysis of plants grown in P 32 (P 32 plant) and P 37 plant during the first 59 days after planting (DAP). Slow growth, low flowering percentage, or even the death of B. boliviensis ‘Bonefire’ grown in P NaS medium is attributed to high pH and high sulfur concentration. Based on the pH, soluble salts level, and the concentrations of macro- and micro-elements in the medium and leaves, P 45 pellet is the suitable source, if nitrogen is supplied constantly to the optimum level. Pellets prepared following mixing > 30 feather fibers without using glycerol should be further tested.
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Physical and Chemical Properties of Biobased Plastic Resins Containing Chicken Feather Fibers
Mark S. Roh,Gary R. Bauchan,Masud S. Huda 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.1
This study was conducted to (a) characterize bio-plastic pellets containing feather fibers by low temperature-scanning electron microscopy (LT-SEM) and X-Ray diffraction analysis, (b) evaluate growth and flowering of Begonia boliviensis A. DC. ‘Bonfire’ when grown in a medium amended with pellets, and (c) analyze macro- and micro-elements in the medium and plant tissues of begonia. Based on physical and chemical analyses of six types of pellets mixed in the medium, pellets 32 (P 32), 37 (P 37), 40 (P 40), and P NaS (P NaS), P NaS containing sodium sulfite and > 30% glycerol were not suitable and not recommended for future evaluations. Pellets containing glycerol at 10%were not suitable for the use as an ingredient for commercial growing media and P 45 is considered suitable pellets to prepare pots.
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Antimicrobial Activity of Bacteriophage Endolysin Produced in Nicotiana benthamiana Plants
( Natalia Kovalskaya ),( Juli Foster Frey ),( David M. Donovan ),( Gary Bauchan ),( Rosemarie W. Hammond ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1
The increasing spread of antibiotic-resistant pathogens has raised the interest in alternative antimicrobial treatments. In our study, the functionally active gram-negative bacterium bacteriophage CP933 endolysin was produced in Nicotiana benthamiana plants by a combination of transient expression and vacuole targeting strategies, and its antimicrobial activity was investigated. Expression of the cp933 gene in E. coli led to growth inhibition and lysis of the host cells or production of trace amounts of CP933. Cytoplasmic expression of the cp933 gene in plants using Potato virus X-based transient expression vectors (pP2C2S and pGR107) resulted in death of the apical portion of experimental plants. To protect plants against the toxic effects of the CP933 protein, the cp933 coding region was fused at its Nterminus to an N-terminal signal peptide from the potato proteinase inhibitor I to direct CP933 to the delta-type vacuoles. Plants producing the CP933 fusion protein did not exhibit the severe toxic effects seen with the unfused protein and the level of expression was 0.16 mg/g of plant tissue. Antimicrobial assays revealed that, in contrast to gram-negative bacterium E. coli (BL21(DE3)), the gram-positive plant pathogenic bacterium Clavibacter michiganensis was more susceptible to the plant-produced CP933, showing 18% growth inhibition. The results of our experiments demonstrate that the combination of transient expression and protein targeting to the delta vacuoles is a promising approach to produce functionally active proteins that exhibit toxicity when expressed in plant cells.
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