Anti-bacterial and Anti-biofilm Effects of Antimicrobial Peptide on Polymicrobial Infections = 항균 펩타이드의 다균 감염에 대한 항균 및 항 생물막 효과에 관한 연구

목차 (Table of Contents)

• LIST OF TABLES iv
• LIST OF FIGURES v
• LIST OF ABBREVIATIONS vii
• 초 록 ix
• Abstract xi
• Ⅰ. Introduction 1
• Ⅱ. Materials and Methods 3
• 1. Materials 3
• 1.1. Materials 3
• 1.2. Bacterial strains and culture conditions 3
• 2. Methods 4
• 2.1. Peptide 4
• 2.1.1. Design of amino acid sequence and Analysis Physicochemical Properties 4
• 2.1.2. Peptides Synthesis and Purification 4
• 2.1.3. Mass Spectrometry 5
• 2.2. Antimicrobial Activity Assay 5
• 2.2.1. Minimum inhibitory concentration (MIC) Assay 5
• 2.2.2. Minimal bactericidal concentration (MBC) Assay 6
• 2.3. Anti-biofilm Activity Assay 6
• 2.3.1. Biofilm Formation Inhibition Assay 6
• 2.3.2. Visualization of Biofilm Formation Inhibition 7
• 2.4. Bacterial Distribution Analysis of polymicrobial infections 8
• 2.4.1. Strain-specific 16s RNA primer 8
• 2.4.2. Polymicrobial infection models and treated with dCATH and dCATH-A5 9
• 2.4.3. RNA Extraction and cDNA Synthesis 9
• 2.4.4. Quantitative Reverse Transcription PCR (RT-qPCR) 10
• 2.5 Statical Analysis 10
• Ⅲ. Results 12
• 1. Peptide dCATH Design and Characterization 12
• 2. Anti-bacterial Activity of dCATH 15
• 3. Peptide dCATH-A5 Design and Characterization 19
• 4. Anti-bacterial Activity of dCATH-A5 22
• 5. Anti-biofilm Activity of dCATH and dCATH-A5 25
• 6. Analysis of bacterial distribution in polymicrobial infections 37
• Ⅳ. Discussion 42
• Ⅴ. References 46