옻나무 부위별 성분과 종실의 항산화활성에 관한 연구

다국어 초록 (Multilingual Abstract)

Rhus verniciflua (R. verniciflua ) is one of six species of lacquer trees in Korea. Among the parts of R. verniciflua, only bark and xylem have been used as food materials because little information about chemical components of the other parts was known. This study was conducted to
compare chemical components in different parts of R. verniciflua and to evaluate antioxidant potentials of ethanol extracts from the seed. The proximate composition, mineral components, urushiol, total polyphenol, and total flavonoids were compared in different parts of R. verniciflua . And antioxidant substances in the ethanol extracts from the seed of R. verniciflua were isolated by chromatographic separation. The antioxidant activity of the separated fractions were also compared. The results were as follows.
1. The contents of crude protein in the leaf and shoot of R. verniciflua were higher than those of stem and seed.
The crude fiber contents in the stem and seed were higher than the other part of R. verniciflua . The nitrogen free extracts in the parts of R verniciflua were ranged from 16.75% to 42.70%.
2. Among the mineral components in R. verniciflua , the content of potassium was the highest, followed by magnesium, sodium, iron, and manganese.
3. The content of urushiol in the shoots of R. verniciflua was th highest by 2680.05 mg%, followed by leaf(2086.37 mg%), stem (2174.71 mg%), and seed (7.47mg%), the lowest.
4. The contents of total polyphenols in the shoots of R. verniciflua was the highest by 413.14 mg GAE/g, followed by leaf (63.80 mg GAE/g), stem (73.36 mg GAE/g), and seed (55.63 mg GAE/g).
5. The content of total flavonoids in shoots of R. verniciflua was the highest by 177.80 mg QE/g, followed by leaf (163.78 mg QE/g), seed (53.96 mg QE/g), and stem (51.62 mg QE/g).
6. In the assay of antioxidant activities according to ethanol concentration, 50% ethanol was determined as an appropriate extracting solvent.
7. In the result of cytotoxic assay, all treatments of 50% ethanol extracts from Rhus verniciflua did not show cytotoxic activities in a time and dose-dependent manner.
8. Among the solvent partitions of 50% ethanol extracts from R. verniciflua , the ethyl acetate portion showed the highest antioxidant activity. Eleven fractions were obtained from the ethyl acetate portion using silica-gel chromatography. Among them, fraction number(Fr) from
2 to 7 showed high antioxidant activities. Among nine sub-fractions separated from the Fr 2-7, sub-number 4 showed the highest antioxidant activity.
9. The purified 3 fractions were obtained from the sub-number 4 and peak No.2 showed the highest antioxidant activity. The purified compound of peak No.2 was identified as fisetin by the results of
13C-NMR, 1H-NMR and LC-MS.

목차 (Table of Contents)

• Ⅰ. 서 론 1
• Ⅱ. 재료 및 방법 7
• 1. 재료 7
• 2. 실험방법 10
• Ⅲ. 결과 및 고찰 35
• 1. 옻나무 부위별 성분과 생리활성 35
• 2. Ethanol 농도에 따른 옻나무 종실 추출물의 항산화활성 49
• 3. In-vitro test를 통한 세포독성 53
• 4. 옻나무 종실의 항산화 물질 분리 및 활성 55
• 5. 항산화 물질 동정 69
• Ⅳ. 요약 73
• Ⅴ. 참고문헌 77