The Zanthoxylum schinifolium Seed Oil modulates Immune Function under the Biological Safety Level

다국어 초록 (Multilingual Abstract)

Zanthoxylum schinifolium (Z. schinifolium) and the seed oil of Z. schinifolium has been widely used as culinary applications and traditional medicine for epigastric pain in Korea, China, and several Asia nations. The components or extracts of Z. schinifolium have been reported to exhibit various pharmaceutical effects such as antiplatelet aggregation, inhibitory activities against monoamine oxidase, antioxidant and anticancer activities, and anti-inflammatory activity. In this study we got the results that 200 mg/kg/day seed oil of Z. schinifolium oral administration for 13 weeks enhances most immune-related cells and is biological safety. However as B cell population was increased by Z. schinifolium seed oil administration but B cell function was not changed by that, ultimately we deduced that Z. schinifolium seed oil doesn’t have the modulation effect about B cell. To evaluate the biological safety of repeated Z. schinifolium seed oil administration the index of biological safety such as CBC, hematological enzymes, and especially histopathological morphologies was measured but no changes related with Z. schinifolium seed oil administration had been shown.
Conclusively Z. schinifolium seed oil is one of the safe candidates to modulate immunity.

국문 초록 (Abstract)

운향과(Rutaceae)의 산초(Zanthoxylum schinifolium)는 옛 부터 한국, 중국, 그리고 아시아의 여러 국가에서 향신료, 전통 소화기 통증약으로 널리 사용되어 왔다. 산초의 추출물 또는 성분은 혈전응집억제, monoamine oxidase 억제효과, 항산화 및 항암제 효과 그리고 human umbilical vein endothelial cells에서의 항염증 효과가 있는 것으로 보고되고 있다.
200 ㎎/㎏/day 산초 종유를 13주간 경구 투여한 결과 대부분의 면역세포가 증강되는 결과를 얻었다. 그러나 산초 종유의 투여결과 B세포의 숫자는 증가하지만 B세포의 기능은 변화하지 않는 것으로 나타났다. 궁극적으로 산초 종유는 B세포의 면역조절 효과는 갖지 않는 것으로 생각되어진다. 산초 종유의 반복적인 경구투여 결과 평가를 위해 생물학적 안전성의 지표라 할 수 있는 혈액검사, 혈청효소검사, 그리고 특히 조직병리학적 검사를 실시하였으나 별다른 특이점을 찾지 못하였다.
결론적으로 산초 종유는 가장 안전한 면역 조절 후보제 중의 하나이다.

목차 (Table of Contents)

• Ⅰ. Introduction = 1
• Ⅱ. Materials & Methods = 3
• 1. Extraction of Z. schinifolium seed oil = 3
• 2. Experimental Animals = 4
• 3. Biological Toxicity Analysis = 5
• 4. Lymphocyte Proliferation and Cell Surface Molecules Analysis = 6
• Ⅲ. Result = 8
• 1. The seed oil of Z. schinifolium induces concanavalin A(Con-A)-stimulated T cell proliferation and CD3e and CD8a activations but not CD4. = 8
• 2. The seed oil of Z. schinifolium is safe in 13-weeks repeated-dose treatment in mice. = 14
• Ⅳ. Discussion = 25
• Ⅴ. Reference = 27
• Abstract = 30
• 감사의 글 = 32