麥門冬湯과 定喘化痰降氣湯이 알레르기 喘息 모델 흰쥐의 BALF內 免疫細胞 및 血淸 IgE에 미치는 影響 = The effect of Maekmoondongtang and Jeongcheonhwadamgangkitang on immune cell & serum OA-specific IgE in BALF in a rat asthma model

다국어 초록 (Multilingual Abstract)

Background : Maekmoondongtang and Jeongcheonhwadamgangkitang are a herbal medicine which has been used of the traditional therapeutic agent of asthma.
Objective : This study was performed to invastigate the effect of Maekmoondongtang and Jeongcheonhwadamgangkitang on immune Cell & serum OA-specific IgE in broncho-alveolar lavage fluid(BALF) in a rat asthma model.
Material and Methods : Rats were sensitized with OA; at day 1 sensitized group and Maekmoondongtang and Jeongcheonhwadamgangkitang groups were systemically immunized by subcutaneous injection of 1㎎ OA and 300㎎ of Al(OH)3 in a total volume of 2㎖. At the same time, 1㎖ of 0.9% saline containing 6×109 B. pertussis bacilli was injected by i.p. 14 days. after the systemic immunization, rats received local immunization by inhaling 0.9％ saline aerosol containing 2％(wt/vol) OA. A day after local immunization, BAL fluid was collected from the rats. A day after local immunization, rats were orally administered with each of Maekmoondongtang and Jeongcheonhwadamgangkitang extract for 14 days. Lymphocyte, CD4+T-cell CD8+T-cell counts, CD4+/CD8+ ratio in BALF, change of serum OA-specific IgE level, CD4+T-cell CD8+T-cell percentage in the peripheral blood were measured and evaluated.
Results : Maekmoondongtang and Jeongcheonhwadamgangkitang showed a suppressive effect on a rat asthma model. Maekmoondongtang decreased total cell, lymphocyte, CD4+T-cell, CD8+T-cell in BALF, serum OA specific IgE level as compared with the control group, whereas Maekmoondongtang decreased CD4+/CD8+ ratio in BALF with statistical nonsignificance as compared with the control group. Jeongcheonhwadamgangkitang decreased total cell, CD4+T-cell, CD4+/CD8+ ratio in BALF, serum OA specific IgE level, whereas Jeongcheonhwadamgangkitang decreased lymphocyte, CD8+T-cell in BALF with statistical nonsignificance as compared with the control group. CD4+T-cell and CD8+T-cell percentage in peripheral blood were not changed significantly in all the experimental groups.
Conclusion : This study shows that Maekmoondongtang and Jeongcheon- hwadamgangkitang have a suppressive effect on asthma. Maekmoondongtang, Jeongcheonhwadamgangkitang would be useful asthma treatment agent.

목차 (Table of Contents)

• Ⅰ. 서 론 = 1
• Ⅱ. 실 험 = 3
• 1. 동물 및 재료 = 3
• 1) 동물 = 3
• 2) 재료 = 3
• (1) 약재 = 3
• (2) 시약 = 5
• 2. 방법 = 5
• 1) 실험동물군 분류 = 5
• 2) 검액의 준비 = 5
• 3) 알레르기 천식 유발 = 6
• 4) 검액 투여 = 6
• 5) 채혈 = 6
• 6) BAL(Broncho- alveolar lavage)과 BALF(BAL fluid) 내의 세포관찰 = 6
• 7) BALF내 CD4+ T세포수와 CD8+ T 세포수의 측정 = 7
• 8) 말초 혈액내 CD4+ T 세포율의 변화 = 7
• 9) 말초 혈액내 CD8+ T 세포율의 변화 = 8
• 10) OA- specific IgE antibody assay = 10
• 3. 통계분석 = 11
• Ⅲ. 성 적 = 12
• 1. Broncho- alveolar lavage fluid(BALF)내의 total cell 수의 변화 = 12
• 2. Broncho- alveolar lavage fluid(BALF)내의 lymphocyte 수의 변화 = 15
• 3. Broncho- alveolar lavage fluid(BALF)내의 CD4+ T 세포수의 변화 = 18
• 4. Broncho- alveolar lavage fluid(BALF)내의 CD8+ T 세포수의 변화 = 22
• 5. Broncho- alveolar lavage fluid(BALF)내의 CD4+/CD8+ 비율의 변화 = 26
• 6. 혈청내 IgE 양의 변화 = 29
• 7. 말초혈액내의 CD4+ T세포율의 변화 = 32
• 8. 말초혈액내의 CD8+ T세포율의 변화 = 35
• Ⅳ. 고 찰 = 38
• Ⅴ. 결 론 = 43
• 참 고 문 헌 = 45
• 영 문 초 록 = 51