토성이 토마토의 생육과 저장성에 미치는 영향 및 부위와 성숙시기별 생리활성 스크리닝 = Growth and storage of Tomato affected by different soil texture and effect of different ripened fruits extracts on cell viability and No production

다국어 초록 (Multilingual Abstract)

Abundant evidence supports that consumption of tomatoes (Solanum lycopersicum) and related food products reduces the incidence of diseases such as chronic degenerative diseases, breast cancer, prostate cancer and anti-inflammation in human health. To our knowledge, this is the first report attempt to compare anti-cancer and anti-inflammatory activities of tomato extracts in different part.
In the present study, we examined the anti-inflammation and anti-cancer effect of extracts of tomato from different parts. The aim of this study was effect of soil texture on growth and storage of Solanum lycopersicum.
The effects of soil texture on growth characteristics (stem height, plant height, leaf length, mean weight, total soluble-solid content, green fruit no./plant, percent of red fruit, red fruit no./plant, percent of red fruit) of Solanum lycopersicum was investigated.
The result of growth characteristic showed that stem height and plant height are more growed in the clay soil in that order sandy clay soil. Leaf length was more growed in the sand soil, in that order sandy clay soil. Total soluble-solid content, green fruit no./plant, percent of red fruit, red fruit no./plant, percent of red fruit were highest in clay soil or sand soil, but not in mean fresh wight of fruits.
This study also showed the effect of fruit obtained from different soil texture on fruit storage quality(respiration, ethylene production, Hunter L, a, b, firmness, total soluble-solid content, decay). Generally, storage quality is good in the sandy clay soil.
To evaluate the anti-inflammatory effects of different maturity stage of fruits by extract solvent( 95%, 75%, 50% pretanol and distilled water) of tomato, the inhibitory effects of tomato extracts on the production of NO were investigated in LPS-stimulated RAW264.7 cells. LPS treatment resulted in a markedly increased production of NO in RAW264.7 cells. However, different concentrations of tomato extracts attenuate LPS-induced overproduction of NO in RAW264.7 cells, especially the extracts of TF5 and TF6 with 95% pretanol, TF2 and TF3 with 75% pretanol, TF1 and TF2 with 50% pretanol and TF1 and TF6 with distilled water were in LPS-induced NO production in Raw264.7 cells.
To evaluate the anti-cancer effects of different maturity stage of fruits by solvent extracts (95%, 75%, 50% pretanol and distilled water) of tomato, the inhibitory effects of tomato extracts on the cell viabilities were examined in AGS cells and HCT-116 cells. These extracts showed significant growth inhibitory effects on AGS cells in a dose-dependent manner, as well as on HCT-116 cells. Especially, the extracts of TF3 with 95% pretanol, TF2 and TF3 with 75% pretanol, TF1 and TF2 with 50% pretanol and TF6 with distilled water showed significant growth inhibitor effects on AGS cells and HCT-116 cells.

목차 (Table of Contents)

• Ⅰ. 서언 1
• Ⅱ. 연구사 3
• 1. 염류와 과실 품질 3
• 2. 염류와 생육 및 수량 4
• 3. 토성에 따른 토마토의 생육 4
• 4. 항염증 효과 6
• 5. 항암 효과 8
• Ⅲ. 재료 및 방법 9
• 1. 공시재료 9
• 2. 토양 처리 9
• 3. 토마토의 생육 조사 10
• 4. 토성이 다른 조건에서 생산한 토마토의 저장성 10
• 1) 호흡력과 에틸렌 생성량 10
• 2) Hunter 값 10
• 3) 경도, 가용성 당 함량 및 부패율 11
• 5. 과일 성숙 시기별 추출물의 항암효과 11
• 1) 시약준비 11
• 2) Cell culture 11
• 3) 추출물 제조 12
• 4) Nitric Oxide 소거능 측정 12
• 5) MTT assay 13
• Ⅳ. 결과 및 고찰 14
• 1. 토마토의 생육 조사 14
• 1) 토성이 토마토의 생육에 미치는 영향 14
• 2) 토성이 토마토의 수량 및 품질에 미치는 영향 17
• 2. 토성이 다른 조건에서 생산한 토마토의 저장성 21
• 1) 호흡력과 에틸렌 생성량 21
• 2) Hunter 값 25
• 3) 경도, 가용성 당 함량 및 부패율 30
• 3. 성숙시기가 다른 토마토 추출물의 항염증 효과 35
• 4. 토마토 과일의 추출용매가 NO 생성에 미치는 영향 36
• 5. 토마토 과일 성숙시기별 추출물의 항암 효과 37
• 인용문헌 39
• ABSTRACT 47