용암해수에서 배양한 감태추출물의 미백효과 및 화장품 제형으로의 응용

다국어 초록 (Multilingual Abstract)

Ecklonia cava is popular in Korea as a marine functional materials. E. cava is generally collected and used on the coast of Jeju Island. However, the continuous use of collected natural E. cava may be limited because difficult to secure throughout the year and may be exposed to environmental pollution. Jeju magma seawater (MSW) was known to be significant advantages such as safety, cleanness, stability, and functional improvement. Attempts have been reported on application of magma seawater to the culturing of macro-and micro algae and showed improved results. Thus, the objective of the present study was to explore the anti-melanogenesis activity of brown seaweed E. cava (MSWE) extract cultured in tanks with magma seawater of Jeju Island to evaluate the possibility of functional cosmetic industrial application. MSWE extract showed the higher polyphenolic and dieckol contents than natural E. cava (NE) extract. Anti-melanogenesis activity of MSWE extract and NE extract was compared using tyrosinase and DOPA oxidation inhibition assay. MSWE extracts evidenced more effective tyrosinase and DOPA oxidation inhibition activity than those of the NE extracts and the commercial whitening agent, arbutin. MSWE extracts also markedly inhibited melanin synthesis and decreased the expression of melanogenesis-related protein in α-MSH-stimulated B16F10 melanoma cells without cytotoxicity. These results suggest that MSWE extract has more bioactive whitening compounds, and thus could be more effective for industrial application.

목차 (Table of Contents)

• I. 서 론 1
• 1. 기능성화장품시장동향 1
• 2. 천연물 소재를 활용한 기능성화장품시장동향 6
• 3. 연구 개발배경 10
• 4. 연구목적 17
• II. 재료 및 방법 18
• 1. 실험재료 18
• 1-1. 시료 및 추출 18
• 2. 실험방법 18
• 2-1. 총 폴리페놀 함량 분석 18
• 2-2. HPLC를 이용한 지표물질 정성․정량 분석 19
• 2-3. Tyrosinase 활성 저해 효과 측정 19
• 2-4. DOPA 산화 반응 저해 효과 측정 20
• 2-5. 세포 배양 20
• 2-6. 세포 독성 측정 21
• 2-7. 멜라닌 합성 저해 활성 측정 21
• 2-8. Western blot analysis를 이용한 Tyrosinase, TRP-1, TRP-2, MITF 발현 저해 효과 측정 22
• 2-9. 화장품 제형 시험 23
• 2-10. 통계학적 방법 29
• III. 결 과 30
• 1. 총 폴리페놀 함량 분석 결과 30
• 2. HPLC를 이용한 지표 물질 정성․정량 분석 결과 32
• 3. 용암해수에서 배양한 감태추출물의 미백효과 측정 결과 34
• 3-1. Tyrosinase 활성 저해 시험 결과 34
• 3-2. DOPA 산화반응 저해 시험 결과 36
• 3-3. 세포독성 시험 결과 38
• 3-4. 멜라닌 합성 저해 활성 결과 40
• 3-5. Western blot analysis를 이용한 Tyrosinase, TRP-1, TRP-2, MITF
• 발현 저해 효과 측정 결과 42
• 4. 화장품 제형으로의 적용 45
• 4-1. 감태추출물 함량별 제형 적용 시험 결과 45
• 4-2. 감태추출물 함량별 제형 적용 후 안정성 시험 결과 54
• IV. 고 찰 76
• V. 참고문헌 79